A mechanism for tunable autoinhibition in the structure of a human Ca 2+/calmodulin-dependent kinase II holoenzyme

Luke H. Chao, Margaret M. Stratton, Il Hyung Lee, Oren S. Rosenberg, Joshua Levitz, Daniel J. Mandell, Tanja Kortemme, Jay T. Groves, Howard Schulman, John Kuriyan

Research output: Contribution to journalArticlepeer-review

155 Scopus citations

Abstract

Calcium/calmodulin-dependent kinase II (CaMKII) forms a highly conserved dodecameric assembly that is sensitive to the frequency of calcium pulse trains. Neither the structure of the dodecameric assembly nor how it regulates CaMKII are known. We present the crystal structure of an autoinhibited full-length human CaMKII holoenzyme, revealing an unexpected compact arrangement of kinase domains docked against a central hub, with the calmodulin-binding sites completely inaccessible. We show that this compact docking is important for the autoinhibition of the kinase domains and for setting the calcium response of the holoenzyme. Comparison of CaMKII isoforms, which differ in the length of the linker between the kinase domain and the hub, demonstrates that these interactions can be strengthened or weakened by changes in linker length. This equilibrium between autoinhibited states provides a simple mechanism for tuning the calcium response without changes in either the hub or the kinase domains. PaperFlick:

Original languageEnglish
Pages (from-to)732-745
Number of pages14
JournalCell
Volume146
Issue number5
DOIs
StatePublished - 2 Sep 2011

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