A novel auxin conjugate hydrolase from wheat with substrate specificity for longer side-chain auxin amide conjugates

James J. Campanella, Adebanke F. Olajide, Volker Magnus, Jutta Ludwig-Müller

Research output: Contribution to journalArticle

40 Scopus citations

Abstract

This study investigates how the ILR1-like indole acetic acid (IAA) amidohydrolase family of genes has functionally evolved in the monocotyledonous species wheat (Triticum aestivum). An ortholog for the Arabidopsis IAR3 auxin amidohydrolase gene has been isolated from wheat (TaIAR3). The TaIAR3 protein hydrolyzes negligible levels of IAA-Ala and no other IAA amino acid conjugates tested, unlike its ortholog IAR3. Instead, TaIAR3 has low specificity for the ester conjugates IAA-Glc and IAA-myoinositol and high specificity for the conjugates of indole-3-butyric acid (IBA-Ala and IBA-Gly) and indole-3-propionic-acid (IPA-Ala) so far tested. TaIAR3 did not convert the methyl esters of the IBA conjugates with Ala and Gly. IBA and IBA conjugates were detected in wheat seedlings by gas chromatography-mass spectrometry, where the conjugate of IBA with Ala may serve as a natural substrate for this enzyme. Endogenous IPA and IPA conjugates were not detected in the seedlings. Additionally, crude protein extracts of wheat seedlings possess auxin amidohydrolase activity. Temporal expression studies of TaIAR3 indicate that the transcript is initially expressed at day 1 after germination. Expression decreases through days 2, 5, 10, 15, and 20. Spatial expression studies found similar levels of expression throughout all wheat tissues examined.

Original languageEnglish
Pages (from-to)2230-2240
Number of pages11
JournalPlant Physiology
Volume135
Issue number4
DOIs
StatePublished - 1 Aug 2004

Fingerprint Dive into the research topics of 'A novel auxin conjugate hydrolase from wheat with substrate specificity for longer side-chain auxin amide conjugates'. Together they form a unique fingerprint.

  • Cite this