A novel auxin conjugate hydrolase from wheat with substrate specificity for longer side-chain auxin amide conjugates

James Campanella, Adebanke F. Olajide, Volker Magnus, Jutta Ludwig-Müller

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38 Citations (Scopus)

Abstract

This study investigates how the ILR1-like indole acetic acid (IAA) amidohydrolase family of genes has functionally evolved in the monocotyledonous species wheat (Triticum aestivum). An ortholog for the Arabidopsis IAR3 auxin amidohydrolase gene has been isolated from wheat (TaIAR3). The TaIAR3 protein hydrolyzes negligible levels of IAA-Ala and no other IAA amino acid conjugates tested, unlike its ortholog IAR3. Instead, TaIAR3 has low specificity for the ester conjugates IAA-Glc and IAA-myoinositol and high specificity for the conjugates of indole-3-butyric acid (IBA-Ala and IBA-Gly) and indole-3-propionic-acid (IPA-Ala) so far tested. TaIAR3 did not convert the methyl esters of the IBA conjugates with Ala and Gly. IBA and IBA conjugates were detected in wheat seedlings by gas chromatography-mass spectrometry, where the conjugate of IBA with Ala may serve as a natural substrate for this enzyme. Endogenous IPA and IPA conjugates were not detected in the seedlings. Additionally, crude protein extracts of wheat seedlings possess auxin amidohydrolase activity. Temporal expression studies of TaIAR3 indicate that the transcript is initially expressed at day 1 after germination. Expression decreases through days 2, 5, 10, 15, and 20. Spatial expression studies found similar levels of expression throughout all wheat tissues examined.

Original languageEnglish
Pages (from-to)2230-2240
Number of pages11
JournalPlant Physiology
Volume135
Issue number4
DOIs
StatePublished - 1 Aug 2004

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Indoleacetic Acids
Hydrolases
substrate specificity
Substrate Specificity
amides
hydrolases
indole butyric acid
Triticum
auxins
Amidohydrolases
indole acetic acid
amide hydrolases
wheat
Seedlings
Esters
seedlings
esters
Inositol
Germination
Complex Mixtures

Cite this

Campanella, James ; Olajide, Adebanke F. ; Magnus, Volker ; Ludwig-Müller, Jutta. / A novel auxin conjugate hydrolase from wheat with substrate specificity for longer side-chain auxin amide conjugates. In: Plant Physiology. 2004 ; Vol. 135, No. 4. pp. 2230-2240.
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A novel auxin conjugate hydrolase from wheat with substrate specificity for longer side-chain auxin amide conjugates. / Campanella, James; Olajide, Adebanke F.; Magnus, Volker; Ludwig-Müller, Jutta.

In: Plant Physiology, Vol. 135, No. 4, 01.08.2004, p. 2230-2240.

Research output: Contribution to journalArticleResearchpeer-review

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