Abstract
A method for the rapid isolation of mitochondrial DNA from the yeast Saccharomyces cerevisiae is described. Cells are first disrupted by vortexing with glass beads and the mitochondiral DNA is then extracted directly from the cell lysate by poly-l-lysine-kieselguhr-exchange chromatography. The method is unique from most other published procedures in that there is no requirement for the isolation of either a crude or purified mitochondrial preparation. Mitochondrial DNA isolated by this procedure is shown to yield restriction endonuclease fragment patterns identical to those obtained from DNA isolated by other previously reported procedures.
| Original language | English |
|---|---|
| Pages (from-to) | 47-53 |
| Number of pages | 7 |
| Journal | Analytical Biochemistry |
| Volume | 128 |
| Issue number | 1 |
| DOIs | |
| State | Published - 1 Jan 1983 |
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A rapid procedure for the isolation of yeast mitochondrial DNA suitable for restriction fragment analysis. / Lee, Lee; Blamire, John; Cottrell, Stephen F.
In: Analytical Biochemistry, Vol. 128, No. 1, 01.01.1983, p. 47-53.Research output: Contribution to journal › Article
TY - JOUR
T1 - A rapid procedure for the isolation of yeast mitochondrial DNA suitable for restriction fragment analysis
AU - Lee, Lee
AU - Blamire, John
AU - Cottrell, Stephen F.
PY - 1983/1/1
Y1 - 1983/1/1
N2 - A method for the rapid isolation of mitochondrial DNA from the yeast Saccharomyces cerevisiae is described. Cells are first disrupted by vortexing with glass beads and the mitochondiral DNA is then extracted directly from the cell lysate by poly-l-lysine-kieselguhr-exchange chromatography. The method is unique from most other published procedures in that there is no requirement for the isolation of either a crude or purified mitochondrial preparation. Mitochondrial DNA isolated by this procedure is shown to yield restriction endonuclease fragment patterns identical to those obtained from DNA isolated by other previously reported procedures.
AB - A method for the rapid isolation of mitochondrial DNA from the yeast Saccharomyces cerevisiae is described. Cells are first disrupted by vortexing with glass beads and the mitochondiral DNA is then extracted directly from the cell lysate by poly-l-lysine-kieselguhr-exchange chromatography. The method is unique from most other published procedures in that there is no requirement for the isolation of either a crude or purified mitochondrial preparation. Mitochondrial DNA isolated by this procedure is shown to yield restriction endonuclease fragment patterns identical to those obtained from DNA isolated by other previously reported procedures.
UR - http://www.scopus.com/inward/record.url?scp=0020652826&partnerID=8YFLogxK
U2 - 10.1016/0003-2697(83)90342-1
DO - 10.1016/0003-2697(83)90342-1
M3 - Article
C2 - 6303155
AN - SCOPUS:0020652826
VL - 128
SP - 47
EP - 53
JO - Analytical Biochemistry
JF - Analytical Biochemistry
SN - 0003-2697
IS - 1
ER -