Altering protein specificity: Techniques and applications

Nina Goodey, Stephen F. Martin

Research output: Contribution to journalReview article

47 Citations (Scopus)

Abstract

Protein engineering constitutes a powerful tool for generating novel proteins that serve as catalysts to induce selective chemical and biological transformations that would not otherwise be possible. Protocols that are commonly employed for altering the substrate specificity and selectivity profiles by mutating known enzymes include rational and random methods as well as techniques that entail evolution, selection and screening. Proteins identified by these techniques play important roles in a variety of industrial and medicinal applications and in the study of protein structure-function relationships. Herein we present a critical overview of methods for creating new functional proteins having altered specificity profiles and some practical case studies in which these techniques have been applied to solving problems in synthetic and medicinal chemistry and to elucidating enzyme function and biological pathways.

Original languageEnglish
Pages (from-to)2701-2716
Number of pages16
JournalBioorganic and Medicinal Chemistry
Volume13
Issue number8
DOIs
StatePublished - 15 Apr 2005

Fingerprint

Proteins
Protein Engineering
Pharmaceutical Chemistry
Enzymes
Substrate Specificity
Screening
Catalysts
Substrates

Keywords

  • Enzyme specificity
  • Mutagenesis
  • Protein engineering

Cite this

@article{0d981db222bb495b8eb422be88efd7a1,
title = "Altering protein specificity: Techniques and applications",
abstract = "Protein engineering constitutes a powerful tool for generating novel proteins that serve as catalysts to induce selective chemical and biological transformations that would not otherwise be possible. Protocols that are commonly employed for altering the substrate specificity and selectivity profiles by mutating known enzymes include rational and random methods as well as techniques that entail evolution, selection and screening. Proteins identified by these techniques play important roles in a variety of industrial and medicinal applications and in the study of protein structure-function relationships. Herein we present a critical overview of methods for creating new functional proteins having altered specificity profiles and some practical case studies in which these techniques have been applied to solving problems in synthetic and medicinal chemistry and to elucidating enzyme function and biological pathways.",
keywords = "Enzyme specificity, Mutagenesis, Protein engineering",
author = "Nina Goodey and Martin, {Stephen F.}",
year = "2005",
month = "4",
day = "15",
doi = "10.1016/j.bmc.2005.01.059",
language = "English",
volume = "13",
pages = "2701--2716",
journal = "Bioorganic and Medicinal Chemistry",
issn = "0968-0896",
publisher = "Elsevier Ltd",
number = "8",

}

Altering protein specificity : Techniques and applications. / Goodey, Nina; Martin, Stephen F.

In: Bioorganic and Medicinal Chemistry, Vol. 13, No. 8, 15.04.2005, p. 2701-2716.

Research output: Contribution to journalReview article

TY - JOUR

T1 - Altering protein specificity

T2 - Techniques and applications

AU - Goodey, Nina

AU - Martin, Stephen F.

PY - 2005/4/15

Y1 - 2005/4/15

N2 - Protein engineering constitutes a powerful tool for generating novel proteins that serve as catalysts to induce selective chemical and biological transformations that would not otherwise be possible. Protocols that are commonly employed for altering the substrate specificity and selectivity profiles by mutating known enzymes include rational and random methods as well as techniques that entail evolution, selection and screening. Proteins identified by these techniques play important roles in a variety of industrial and medicinal applications and in the study of protein structure-function relationships. Herein we present a critical overview of methods for creating new functional proteins having altered specificity profiles and some practical case studies in which these techniques have been applied to solving problems in synthetic and medicinal chemistry and to elucidating enzyme function and biological pathways.

AB - Protein engineering constitutes a powerful tool for generating novel proteins that serve as catalysts to induce selective chemical and biological transformations that would not otherwise be possible. Protocols that are commonly employed for altering the substrate specificity and selectivity profiles by mutating known enzymes include rational and random methods as well as techniques that entail evolution, selection and screening. Proteins identified by these techniques play important roles in a variety of industrial and medicinal applications and in the study of protein structure-function relationships. Herein we present a critical overview of methods for creating new functional proteins having altered specificity profiles and some practical case studies in which these techniques have been applied to solving problems in synthetic and medicinal chemistry and to elucidating enzyme function and biological pathways.

KW - Enzyme specificity

KW - Mutagenesis

KW - Protein engineering

UR - http://www.scopus.com/inward/record.url?scp=15244340939&partnerID=8YFLogxK

U2 - 10.1016/j.bmc.2005.01.059

DO - 10.1016/j.bmc.2005.01.059

M3 - Review article

C2 - 15781382

AN - SCOPUS:15244340939

VL - 13

SP - 2701

EP - 2716

JO - Bioorganic and Medicinal Chemistry

JF - Bioorganic and Medicinal Chemistry

SN - 0968-0896

IS - 8

ER -