Altering substrate specificity of phosphatidylcholine-preferring phospholipase C of Bacillus cereus by random mutagenesis of the headgroup binding site

Keyphrases

• Substrate Specificity 100%
• Binding Site 100%
• Phosphatidylcholine 100%
• Head Group 100%
• Bacillus Cereus (B. cereus) 100%
• Random mutagenesis 100%
• Phospholipase C 100%
• Specificity Constant 80%
• High Specificity 60%
• Charged Residue 40%
• Binding Pocket 40%
• Fusion Protein 40%
• Hydrolysis 20%
• Hydrolytic Activity 20%
• In Situ 20%
• Catalytic Activity 20%
• Site-directed mutagenesis 20%
• Altered Specificity 20%
• Phospholipids 20%
• Mutant Enzyme 20%
• Negatively Charged 20%
• Triethanolamine 20%
• Amino Acid Residues 20%
• Random Permutation 20%
• 96-Well Format 20%
• Phosphodiester Bond 20%
• Maltose-binding Protein 20%
• Diacylglycerol 20%
• Novel Protocol 20%
• Steady-state Kinetics 20%
• Protein mutants 20%
• Positively Charged 20%
• High Catalytic Activity 20%
• Combinatorial Library 20%
• Monomeric Enzymes 20%
• Phosphatidylethanolamine 20%
• Soluble Substrate 20%
• Enzyme Specificity 20%
• Aromatic Residues 20%

Biochemistry, Genetics and Molecular Biology

• Enzyme 100%
• Binding Site 100%
• Mutagenesis 100%
• Enzyme Specificity 100%
• Bacillus Cereus 100%
• Specificity Constant 100%
• Lecithinase 100%
• Lecithin 100%
• Enzymatic Hydrolysis 50%
• Enzymatic Activity 50%
• Chimeric Protein 50%
• Amino Acids 25%
• Hydrolase 25%
• Biochemistry 25%
• Steady State 25%
• Site-Directed Mutagenesis 25%
• Maltose-Binding Protein 25%
• Phosphodiester Bond 25%
• Phosphatidylserine 25%
• Mutant Protein 25%
• Combinatorial Library 25%
• Serine 25%
• Diglyceride 25%
• Phosphatidylethanolamine 25%
• Phospholipid 25%

Immunology and Microbiology

• Mutagenesis 100%
• Enzyme Specificity 100%
• Bacillus Cereus 100%
• Binding Site 100%
• Protein Binding 25%
• Steady State 25%
• Site Directed Mutagenesis 25%
• Maltose Binding 25%
• Combinatorial Library 25%
• Amino Acid 25%
• Serine 25%