An enzymatic analysis of loblolly pine and Sitka spruce auxin conjugate hydrolases and evolutionary implications

James Campanella, N. Zaben, D. Enriquez, J. V. Smalley, J. Ludwig-Müller

Research output: Chapter in Book/Report/Conference proceedingChapterResearchpeer-review

3 Citations (Scopus)

Abstract

We have examined how the ILR1-like auxin conjugate hydrolase gene family has functionally evolved in the gymnosperm species Sitka spruce (Picea sitchensis) and Loblolly pine (Pinus taeda). We have isolated and cloned two orthologues from spruce (PsIAR31, PsIAR32) and one from pine (PtIAR31) that are homologous to the Arabidopsis thaliana AtIAR3 auxin amidohydrolase. We have previously examined the enzymatic activity of PsIAR31 using a thin layer chromatography method. Using more sensitive HPLC methods, we have re-investigated the hydrolytic activity and substrate recognition of both PsIAR31 and PsIAR32. Neither PsIAR31 nor PsIAR32 appears to hydrolyze indole butyric acid (IBA) or indole proprionic acid (IPA) conjugates. Additionally, we have found that PsIAR31 and -32 recognize several IAA conjugates (including IAA-ala, IAA-asp, IAA-gluc, and IAA-isoleu). The PtIAR31 enzyme seems to primarily recognize IBA-alanine as a substrate. Using phylogenetic analysis, we also found a family of five putative paralogue hydrolases in both the pine (PtIAR31-35) and the spruce (PsIAR31-35) genomes. This result supports the hypothesis that multiple copies of auxin conjugate hydrolase genes have been present since gymnosperms evolved, and that this redundancy did not arise later in angiosperms.

Original languageEnglish
Title of host publicationXII International Symposium on Plant Bioregulators in Fruit Production
EditorsS.J. McArtney, T. Spann
PublisherInternational Society for Horticultural Science
Pages79-87
Number of pages9
ISBN (Electronic)9789462610316
StatePublished - 20 Jul 2014

Publication series

NameActa Horticulturae
Volume1042
ISSN (Print)0567-7572

Fingerprint

Picea sitchensis
Pinus taeda
hydrolases
indole acetic acid
auxins
indole butyric acid
Picea
Pinus
amide hydrolases
indoles
thin layer chromatography
alanine
Angiospermae
genes
Arabidopsis thaliana
high performance liquid chromatography
genome
acids
phylogeny
enzymes

Keywords

  • Auxin
  • Gymnosperms
  • Picea sitchensis
  • Pinus taeda

Cite this

Campanella, J., Zaben, N., Enriquez, D., Smalley, J. V., & Ludwig-Müller, J. (2014). An enzymatic analysis of loblolly pine and Sitka spruce auxin conjugate hydrolases and evolutionary implications. In S. J. McArtney, & T. Spann (Eds.), XII International Symposium on Plant Bioregulators in Fruit Production (pp. 79-87). (Acta Horticulturae; Vol. 1042). International Society for Horticultural Science.
Campanella, James ; Zaben, N. ; Enriquez, D. ; Smalley, J. V. ; Ludwig-Müller, J. / An enzymatic analysis of loblolly pine and Sitka spruce auxin conjugate hydrolases and evolutionary implications. XII International Symposium on Plant Bioregulators in Fruit Production. editor / S.J. McArtney ; T. Spann. International Society for Horticultural Science, 2014. pp. 79-87 (Acta Horticulturae).
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abstract = "We have examined how the ILR1-like auxin conjugate hydrolase gene family has functionally evolved in the gymnosperm species Sitka spruce (Picea sitchensis) and Loblolly pine (Pinus taeda). We have isolated and cloned two orthologues from spruce (PsIAR31, PsIAR32) and one from pine (PtIAR31) that are homologous to the Arabidopsis thaliana AtIAR3 auxin amidohydrolase. We have previously examined the enzymatic activity of PsIAR31 using a thin layer chromatography method. Using more sensitive HPLC methods, we have re-investigated the hydrolytic activity and substrate recognition of both PsIAR31 and PsIAR32. Neither PsIAR31 nor PsIAR32 appears to hydrolyze indole butyric acid (IBA) or indole proprionic acid (IPA) conjugates. Additionally, we have found that PsIAR31 and -32 recognize several IAA conjugates (including IAA-ala, IAA-asp, IAA-gluc, and IAA-isoleu). The PtIAR31 enzyme seems to primarily recognize IBA-alanine as a substrate. Using phylogenetic analysis, we also found a family of five putative paralogue hydrolases in both the pine (PtIAR31-35) and the spruce (PsIAR31-35) genomes. This result supports the hypothesis that multiple copies of auxin conjugate hydrolase genes have been present since gymnosperms evolved, and that this redundancy did not arise later in angiosperms.",
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Campanella, J, Zaben, N, Enriquez, D, Smalley, JV & Ludwig-Müller, J 2014, An enzymatic analysis of loblolly pine and Sitka spruce auxin conjugate hydrolases and evolutionary implications. in SJ McArtney & T Spann (eds), XII International Symposium on Plant Bioregulators in Fruit Production. Acta Horticulturae, vol. 1042, International Society for Horticultural Science, pp. 79-87.

An enzymatic analysis of loblolly pine and Sitka spruce auxin conjugate hydrolases and evolutionary implications. / Campanella, James; Zaben, N.; Enriquez, D.; Smalley, J. V.; Ludwig-Müller, J.

XII International Symposium on Plant Bioregulators in Fruit Production. ed. / S.J. McArtney; T. Spann. International Society for Horticultural Science, 2014. p. 79-87 (Acta Horticulturae; Vol. 1042).

Research output: Chapter in Book/Report/Conference proceedingChapterResearchpeer-review

TY - CHAP

T1 - An enzymatic analysis of loblolly pine and Sitka spruce auxin conjugate hydrolases and evolutionary implications

AU - Campanella, James

AU - Zaben, N.

AU - Enriquez, D.

AU - Smalley, J. V.

AU - Ludwig-Müller, J.

PY - 2014/7/20

Y1 - 2014/7/20

N2 - We have examined how the ILR1-like auxin conjugate hydrolase gene family has functionally evolved in the gymnosperm species Sitka spruce (Picea sitchensis) and Loblolly pine (Pinus taeda). We have isolated and cloned two orthologues from spruce (PsIAR31, PsIAR32) and one from pine (PtIAR31) that are homologous to the Arabidopsis thaliana AtIAR3 auxin amidohydrolase. We have previously examined the enzymatic activity of PsIAR31 using a thin layer chromatography method. Using more sensitive HPLC methods, we have re-investigated the hydrolytic activity and substrate recognition of both PsIAR31 and PsIAR32. Neither PsIAR31 nor PsIAR32 appears to hydrolyze indole butyric acid (IBA) or indole proprionic acid (IPA) conjugates. Additionally, we have found that PsIAR31 and -32 recognize several IAA conjugates (including IAA-ala, IAA-asp, IAA-gluc, and IAA-isoleu). The PtIAR31 enzyme seems to primarily recognize IBA-alanine as a substrate. Using phylogenetic analysis, we also found a family of five putative paralogue hydrolases in both the pine (PtIAR31-35) and the spruce (PsIAR31-35) genomes. This result supports the hypothesis that multiple copies of auxin conjugate hydrolase genes have been present since gymnosperms evolved, and that this redundancy did not arise later in angiosperms.

AB - We have examined how the ILR1-like auxin conjugate hydrolase gene family has functionally evolved in the gymnosperm species Sitka spruce (Picea sitchensis) and Loblolly pine (Pinus taeda). We have isolated and cloned two orthologues from spruce (PsIAR31, PsIAR32) and one from pine (PtIAR31) that are homologous to the Arabidopsis thaliana AtIAR3 auxin amidohydrolase. We have previously examined the enzymatic activity of PsIAR31 using a thin layer chromatography method. Using more sensitive HPLC methods, we have re-investigated the hydrolytic activity and substrate recognition of both PsIAR31 and PsIAR32. Neither PsIAR31 nor PsIAR32 appears to hydrolyze indole butyric acid (IBA) or indole proprionic acid (IPA) conjugates. Additionally, we have found that PsIAR31 and -32 recognize several IAA conjugates (including IAA-ala, IAA-asp, IAA-gluc, and IAA-isoleu). The PtIAR31 enzyme seems to primarily recognize IBA-alanine as a substrate. Using phylogenetic analysis, we also found a family of five putative paralogue hydrolases in both the pine (PtIAR31-35) and the spruce (PsIAR31-35) genomes. This result supports the hypothesis that multiple copies of auxin conjugate hydrolase genes have been present since gymnosperms evolved, and that this redundancy did not arise later in angiosperms.

KW - Auxin

KW - Gymnosperms

KW - Picea sitchensis

KW - Pinus taeda

UR - http://www.scopus.com/inward/record.url?scp=84907978628&partnerID=8YFLogxK

M3 - Chapter

T3 - Acta Horticulturae

SP - 79

EP - 87

BT - XII International Symposium on Plant Bioregulators in Fruit Production

A2 - McArtney, S.J.

A2 - Spann, T.

PB - International Society for Horticultural Science

ER -

Campanella J, Zaben N, Enriquez D, Smalley JV, Ludwig-Müller J. An enzymatic analysis of loblolly pine and Sitka spruce auxin conjugate hydrolases and evolutionary implications. In McArtney SJ, Spann T, editors, XII International Symposium on Plant Bioregulators in Fruit Production. International Society for Horticultural Science. 2014. p. 79-87. (Acta Horticulturae).