Abstract
In the postembryonic zebrafish forebrain, subpial locations of neurogenesis do exist in the early cerebellar external granular layer, and - unusually among vertebrates - in the primordial pretectal (M1) and preglomerular (M2) Anlagen as shown here with 5-bromo-2′-deoxyuridine (BrdU)/Hu-immunocytochemistry and in situ hybridization of neuroD. An intermediate BrdU incubation time of 12-16h reveals in addition to proliferative ventricularly located cells those in M1 and M2. This BrdU saturation-labeling shows - in conjunction with a Hu-assay demonstrating earliest neuronal differentiation - that proliferating cells in M1 and M2 represent neuronal progenitors. This is demonstrated by single BrdU-labeled and double BrdU-/Hu-labeled cells in these aggregates. Further, expression of NeuroD - a marker for freshly determined neuronal cells - confirms this unusual subpial postembryonic forebrain neurogenesis.
| Original language | English |
|---|---|
| Pages (from-to) | 123-135 |
| Number of pages | 13 |
| Journal | Mechanisms of Development |
| Volume | 117 |
| Issue number | 1-2 |
| DOIs | |
| State | Published - Sep 2002 |
Keywords
- 5-Bromo-2′-deoxyuridine
- Cerebellum
- Diencephalon
- External granular layer
- Forebrain
- Hu-protein
- neuroD
- nrd
- Posterior tuberculum
- Preglomerular area
- Pretectum
- Proliferating cell nuclear antigen
- Telencephalon
- Zebrafish
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