Caenorhabditis elegans paraoxonase-like proteins control the functional expression of DEG/ENaC mechanosensory proteins

Yushu Chen, Shashank Bharill, Zeynep Altun, Robert O'Hagan, Brian Coblitz, Ehud Y. Isacoff, Martin Chalfie

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Caenorhabditis elegans senses gentle touch via a mechanotransduction channel formed from the DEG/ENaC proteins MEC-4 and MEC-10. An additional protein, the paraoxonase-like protein MEC-6, is essential for transduction, and previous work suggested that MEC-6 was part of the transduction complex. We found that MEC-6 and a similar protein, POML-1, reside primarily in the endoplasmic reticulum and do not colocalize with MEC-4 on the plasma membrane in vivo. As with MEC-6, POML-1 is needed for touch sensitivity, the neurodegeneration caused by the mec-4(d) mutation, and the expression and distribution of MEC-4 in vivo. Both proteins are likely needed for the proper folding or assembly of MEC-4 channels in vivo as measured by FRET. MEC-6 detectably increases the rate of MEC-4 accumulation on the Xenopus oocyte plasma membrane. These results suggest that MEC-6 and POML-1 interact with MEC-4 to facilitate expression and localization of MEC-4 on the cell surface. Thus MEC-6 and POML-1 act more like chaperones for MEC-4 than channel components.

Original languageEnglish
Pages (from-to)1272-1285
Number of pages14
JournalMolecular Biology of the Cell
Volume27
Issue number8
DOIs
StatePublished - 15 Apr 2016

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Aryldialkylphosphatase
Caenorhabditis elegans
Touch
Proteins
Cell Membrane
Xenopus
Endoplasmic Reticulum
Oocytes
Mutation

Cite this

Chen, Yushu ; Bharill, Shashank ; Altun, Zeynep ; O'Hagan, Robert ; Coblitz, Brian ; Isacoff, Ehud Y. ; Chalfie, Martin. / Caenorhabditis elegans paraoxonase-like proteins control the functional expression of DEG/ENaC mechanosensory proteins. In: Molecular Biology of the Cell. 2016 ; Vol. 27, No. 8. pp. 1272-1285.
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Caenorhabditis elegans paraoxonase-like proteins control the functional expression of DEG/ENaC mechanosensory proteins. / Chen, Yushu; Bharill, Shashank; Altun, Zeynep; O'Hagan, Robert; Coblitz, Brian; Isacoff, Ehud Y.; Chalfie, Martin.

In: Molecular Biology of the Cell, Vol. 27, No. 8, 15.04.2016, p. 1272-1285.

Research output: Contribution to journalArticle

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