Calcium clamp in single nerve cells

P. V. Belan, P. G. Kostyuk, Vladislav Snitsarev, A. V. Tepikin

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Abstract

Free calcium concentration in isolated single neurons was clamped using a new technical approach based on a feed-back connection between the Fura-2 fluorescence signal measuring the intracellular Ca2+ concentration ([Ca2+]i) and iontophoretic current injecting Ca2+ into the cell. Beginning of [Ca2+]i clamping at a level above the basal one triggered fast (few seconds) current transients equal to injection of 36 ± 20 μM Ca2+ (for a 0.1 μM change of [Ca2+]i), representing the filling of a fast cytosolic buffer. Continuation of clamping required very small clamping currents (corresponding to injection of 0.39 ± 0.20 μM.s-1 Ca2+). This value increased proportionally to the magnitude of the change of [Ca2+]i above basal level, indicating the activation of calcium-dependent mechanisms for Ca2+ removal from the cytosol. The described approach allowed measurement, under physiological conditions, of the capacitative and kinetic properties of different Ca-regulating systems functioning in a single nerve cell as well as other types of cells.

Original languageEnglish
Pages (from-to)419-425
Number of pages7
JournalCell Calcium
Volume14
Issue number6
DOIs
StatePublished - 1 Jan 1993

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Constriction
Calcium
Neurons
Injections
Fura-2
Cytosol
Buffers
Fluorescence

Cite this

Belan, P. V., Kostyuk, P. G., Snitsarev, V., & Tepikin, A. V. (1993). Calcium clamp in single nerve cells. Cell Calcium, 14(6), 419-425. https://doi.org/10.1016/0143-4160(93)90001-M
Belan, P. V. ; Kostyuk, P. G. ; Snitsarev, Vladislav ; Tepikin, A. V. / Calcium clamp in single nerve cells. In: Cell Calcium. 1993 ; Vol. 14, No. 6. pp. 419-425.
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Belan, PV, Kostyuk, PG, Snitsarev, V & Tepikin, AV 1993, 'Calcium clamp in single nerve cells', Cell Calcium, vol. 14, no. 6, pp. 419-425. https://doi.org/10.1016/0143-4160(93)90001-M

Calcium clamp in single nerve cells. / Belan, P. V.; Kostyuk, P. G.; Snitsarev, Vladislav; Tepikin, A. V.

In: Cell Calcium, Vol. 14, No. 6, 01.01.1993, p. 419-425.

Research output: Contribution to journalArticleResearchpeer-review

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