Cell cycle regulation of cyclin A gene expression by the cyclic AMP- responsive transcription factors CREB and CREM

C. Desdouets, G. Matesic, Carlos Molina, N. S. Foulkes, P. Sassone-Corsi, C. Brechot, J. Sobczak-Thepot

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Abstract

Cyclin A is a pivotal regulatory protein which, in mammalian cells, is involved in the S phase of the cell cycle. Transcription of the human cyclin A gene is cell cycle regulated. We have investigated the role of the cyclic AMP (cAMP)-dependent signalling pathway in this cell cycle-dependent control. In human diploid fibroblasts (Hs 27), induction of cyclin A gene expression at G1/S is stimulated by 8-bromo-cAMP and suppressed by the protein kinase A inhibitor H89, which was found to delay S phase entry. Transfection experiments showed that the cyclin A promoter is inducible by activation of the adenylyl cyclase signalling pathway. Stimulation is mediated predominantly via a cAMP response element (CRE) located at positions -80 to - 73 with respect to the transcription initiation site and is able to bind CRE- binding proteins and CRE modulators. Moreover, activation by phosphorylation of the activators CRE-binding proteins and CRE modulator τ and levels of the inducible cAMP early repressor are cell cycle regulated, which is consistent with the pattern of cyclin A inducibility by cAMP during the cell cycle. These results suggest that the CRE is, at least partly, implicated in stimulation of cyclin A transcription at G1/S.

Original languageEnglish
Pages (from-to)3301-3309
Number of pages9
JournalMolecular and Cellular Biology
Volume15
Issue number6
DOIs
StatePublished - 1 Jan 1995

Fingerprint

Cyclin A
Cyclic AMP
Cell Cycle
Transcription Factors
Response Elements
Gene Expression
Cyclic AMP Response Element-Binding Protein
S Phase
8-Bromo Cyclic Adenosine Monophosphate
cdc Genes
Transcription Initiation Site
Protein Kinase Inhibitors
Cyclic AMP-Dependent Protein Kinases
Cell Cycle Checkpoints
Diploidy
Adenylyl Cyclases
Transfection
Fibroblasts
Phosphorylation
Proteins

Cite this

Desdouets, C. ; Matesic, G. ; Molina, Carlos ; Foulkes, N. S. ; Sassone-Corsi, P. ; Brechot, C. ; Sobczak-Thepot, J. / Cell cycle regulation of cyclin A gene expression by the cyclic AMP- responsive transcription factors CREB and CREM. In: Molecular and Cellular Biology. 1995 ; Vol. 15, No. 6. pp. 3301-3309.
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title = "Cell cycle regulation of cyclin A gene expression by the cyclic AMP- responsive transcription factors CREB and CREM",
abstract = "Cyclin A is a pivotal regulatory protein which, in mammalian cells, is involved in the S phase of the cell cycle. Transcription of the human cyclin A gene is cell cycle regulated. We have investigated the role of the cyclic AMP (cAMP)-dependent signalling pathway in this cell cycle-dependent control. In human diploid fibroblasts (Hs 27), induction of cyclin A gene expression at G1/S is stimulated by 8-bromo-cAMP and suppressed by the protein kinase A inhibitor H89, which was found to delay S phase entry. Transfection experiments showed that the cyclin A promoter is inducible by activation of the adenylyl cyclase signalling pathway. Stimulation is mediated predominantly via a cAMP response element (CRE) located at positions -80 to - 73 with respect to the transcription initiation site and is able to bind CRE- binding proteins and CRE modulators. Moreover, activation by phosphorylation of the activators CRE-binding proteins and CRE modulator τ and levels of the inducible cAMP early repressor are cell cycle regulated, which is consistent with the pattern of cyclin A inducibility by cAMP during the cell cycle. These results suggest that the CRE is, at least partly, implicated in stimulation of cyclin A transcription at G1/S.",
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Desdouets, C, Matesic, G, Molina, C, Foulkes, NS, Sassone-Corsi, P, Brechot, C & Sobczak-Thepot, J 1995, 'Cell cycle regulation of cyclin A gene expression by the cyclic AMP- responsive transcription factors CREB and CREM', Molecular and Cellular Biology, vol. 15, no. 6, pp. 3301-3309. https://doi.org/10.1128/MCB.15.6.3301

Cell cycle regulation of cyclin A gene expression by the cyclic AMP- responsive transcription factors CREB and CREM. / Desdouets, C.; Matesic, G.; Molina, Carlos; Foulkes, N. S.; Sassone-Corsi, P.; Brechot, C.; Sobczak-Thepot, J.

In: Molecular and Cellular Biology, Vol. 15, No. 6, 01.01.1995, p. 3301-3309.

Research output: Contribution to journalArticleResearchpeer-review

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AU - Desdouets, C.

AU - Matesic, G.

AU - Molina, Carlos

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