CO photolysis of cytochrome oxidase investigated by ps resonance Raman spectroscopy

Johannes P.M. Schelvis; Costas Varotsis; Geurt Deinum; Gerald T. Babcock

doi:10.1155/1999/67252

CO photolysis of cytochrome oxidase investigated by ps resonance Raman spectroscopy

Johannes P.M. Schelvis, Costas Varotsis, Geurt Deinum, Gerald T. Babcock

Chemistry and Biochemistry

Research output: Contribution to journal › Article › peer-review

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Abstract

Low-power picosecond resonance Raman spectroscopy was used to investigate the identity of the axial ligand of heme a₃ and relaxation processes in the heme a₃ pocket of cytochrome oxidase after CO photolysis. Our results show that the proximal histidine remains ligated to heme a₃ after CO photolysis excluding the transient ligation of a photolabile, endogenous ligand. Furthermore, the relaxation of the heme a₃ macrocycle modes occurs on the sub ps time scale, while relaxation of the heme pocket to its equilibrium conformation takes place on the μs time scale.

Original language	English
Pages (from-to)	223-225
Number of pages	3
Journal	Laser Chemistry
Volume	19
Issue number	1-4
DOIs	https://doi.org/10.1155/1999/67252
State	Published - 1999

Keywords

CO
Cytochrome oxidase
Photolysis
Picosecond
Resonance Raman

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10.1155/1999/67252

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title = "CO photolysis of cytochrome oxidase investigated by ps resonance Raman spectroscopy",

abstract = "Low-power picosecond resonance Raman spectroscopy was used to investigate the identity of the axial ligand of heme a3 and relaxation processes in the heme a3 pocket of cytochrome oxidase after CO photolysis. Our results show that the proximal histidine remains ligated to heme a3 after CO photolysis excluding the transient ligation of a photolabile, endogenous ligand. Furthermore, the relaxation of the heme a3 macrocycle modes occurs on the sub ps time scale, while relaxation of the heme pocket to its equilibrium conformation takes place on the μs time scale.",

keywords = "CO, Cytochrome oxidase, Photolysis, Picosecond, Resonance Raman",

author = "Schelvis, {Johannes P.M.} and Costas Varotsis and Geurt Deinum and Babcock, {Gerald T.}",

year = "1999",

doi = "10.1155/1999/67252",

language = "English",

volume = "19",

pages = "223--225",

journal = "Laser Chemistry",

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TY - JOUR

T1 - CO photolysis of cytochrome oxidase investigated by ps resonance Raman spectroscopy

AU - Schelvis, Johannes P.M.

AU - Varotsis, Costas

AU - Deinum, Geurt

AU - Babcock, Gerald T.

PY - 1999

Y1 - 1999

N2 - Low-power picosecond resonance Raman spectroscopy was used to investigate the identity of the axial ligand of heme a3 and relaxation processes in the heme a3 pocket of cytochrome oxidase after CO photolysis. Our results show that the proximal histidine remains ligated to heme a3 after CO photolysis excluding the transient ligation of a photolabile, endogenous ligand. Furthermore, the relaxation of the heme a3 macrocycle modes occurs on the sub ps time scale, while relaxation of the heme pocket to its equilibrium conformation takes place on the μs time scale.

AB - Low-power picosecond resonance Raman spectroscopy was used to investigate the identity of the axial ligand of heme a3 and relaxation processes in the heme a3 pocket of cytochrome oxidase after CO photolysis. Our results show that the proximal histidine remains ligated to heme a3 after CO photolysis excluding the transient ligation of a photolabile, endogenous ligand. Furthermore, the relaxation of the heme a3 macrocycle modes occurs on the sub ps time scale, while relaxation of the heme pocket to its equilibrium conformation takes place on the μs time scale.

KW - CO

KW - Cytochrome oxidase

KW - Photolysis

KW - Picosecond

KW - Resonance Raman

UR - http://www.scopus.com/inward/record.url?scp=0347469472&partnerID=8YFLogxK

U2 - 10.1155/1999/67252

DO - 10.1155/1999/67252

M3 - Article

AN - SCOPUS:0347469472

SN - 0278-6273

VL - 19

SP - 223

EP - 225

JO - Laser Chemistry

JF - Laser Chemistry

IS - 1-4

ER -

CO photolysis of cytochrome oxidase investigated by ps resonance Raman spectroscopy

Abstract

Keywords

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