Correction to: A protocol for custom CRISPR Cas9 donor vector construction to truncate genes in mammalian cells using pcDNA3 backbone (BMC Molecular Biology (2018) 19 (3) DOI: 10.1186/s12867-018-0105-8)

Neftali Vazquez, Lilia Sanchez, Rebecca Marks, Eduardo Martinez, Victor Fanniel, Alma Lopez, Andrea Salinas, Itzel Flores, Jesse Hirschmann, Robert Gilkerson, Erin Schuenzel, Robert Dearth, Reginald Halaby, Wendy Innis-Whitehouse, Megan Keniry

Research output: Contribution to journalComment/debate

Abstract

The original article [1] contains three erroneous mentions of usage of a restriction enzyme-BstZ17I-in the Methods section as displayed in the following sentences: 1. [⋯] Therefore, FOXO3 gene fragments (PCR products) had pcDNA3 sequences on the ends that corresponded to upstream and downstream sequences of the utilized restriction sites (DraIII for Arm1 and BstZ17I for Arm2) in pcDNA3. 2. [⋯] The intermediate vector (with FOXO3 Arm 1) was cut with BstZ17I, which is on the other side of the neomycin resistance gene in the pcDNA3 plasmid compared to FOXO3 Arm 1. 3. [⋯] FOXO3 Arm 2 was amplified with the primer pair specified in Table 1, producing a product that had sequences on each end that were identical to the sequences proximal to the BstZ17I site in the intermediate FOXO3 Arm 1 vector. As such, the above three sentences should instead have stated the correct restriction enzyme-BsmI-in place of where BstZ17I was mentioned in each instance.

Original languageEnglish
Article number20
JournalBMC Molecular Biology
Volume20
Issue number1
DOIs
StatePublished - 14 Aug 2019

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    Vazquez, N., Sanchez, L., Marks, R., Martinez, E., Fanniel, V., Lopez, A., Salinas, A., Flores, I., Hirschmann, J., Gilkerson, R., Schuenzel, E., Dearth, R., Halaby, R., Innis-Whitehouse, W., & Keniry, M. (2019). Correction to: A protocol for custom CRISPR Cas9 donor vector construction to truncate genes in mammalian cells using pcDNA3 backbone (BMC Molecular Biology (2018) 19 (3) DOI: 10.1186/s12867-018-0105-8). BMC Molecular Biology, 20(1), [20]. https://doi.org/10.1186/s12867-019-0138-7