Correction to

A protocol for custom CRISPR Cas9 donor vector construction to truncate genes in mammalian cells using pcDNA3 backbone (BMC Molecular Biology (2018) 19 (3) DOI: 10.1186/s12867-018-0105-8)

Neftali Vazquez, Lilia Sanchez, Rebecca Marks, Eduardo Martinez, Victor Fanniel, Alma Lopez, Andrea Salinas, Itzel Flores, Jesse Hirschmann, Robert Gilkerson, Erin Schuenzel, Robert Dearth, Reginald Halaby, Wendy Innis-Whitehouse, Megan Keniry

Research output: Contribution to journalComment/debateResearch

Abstract

The original article [1] contains three erroneous mentions of usage of a restriction enzyme-BstZ17I-in the Methods section as displayed in the following sentences: 1. [⋯] Therefore, FOXO3 gene fragments (PCR products) had pcDNA3 sequences on the ends that corresponded to upstream and downstream sequences of the utilized restriction sites (DraIII for Arm1 and BstZ17I for Arm2) in pcDNA3. 2. [⋯] The intermediate vector (with FOXO3 Arm 1) was cut with BstZ17I, which is on the other side of the neomycin resistance gene in the pcDNA3 plasmid compared to FOXO3 Arm 1. 3. [⋯] FOXO3 Arm 2 was amplified with the primer pair specified in Table 1, producing a product that had sequences on each end that were identical to the sequences proximal to the BstZ17I site in the intermediate FOXO3 Arm 1 vector. As such, the above three sentences should instead have stated the correct restriction enzyme-BsmI-in place of where BstZ17I was mentioned in each instance.

Original languageEnglish
Article number20
JournalBMC Molecular Biology
Volume20
Issue number1
DOIs
StatePublished - 14 Aug 2019

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Clustered Regularly Interspaced Short Palindromic Repeats
Molecular Biology
Neomycin
Enzymes
Genes
Plasmids
Polymerase Chain Reaction

Cite this

Vazquez, Neftali ; Sanchez, Lilia ; Marks, Rebecca ; Martinez, Eduardo ; Fanniel, Victor ; Lopez, Alma ; Salinas, Andrea ; Flores, Itzel ; Hirschmann, Jesse ; Gilkerson, Robert ; Schuenzel, Erin ; Dearth, Robert ; Halaby, Reginald ; Innis-Whitehouse, Wendy ; Keniry, Megan. / Correction to : A protocol for custom CRISPR Cas9 donor vector construction to truncate genes in mammalian cells using pcDNA3 backbone (BMC Molecular Biology (2018) 19 (3) DOI: 10.1186/s12867-018-0105-8). In: BMC Molecular Biology. 2019 ; Vol. 20, No. 1.
@article{31a46c453e4847b7a84f1339790520ee,
title = "Correction to: A protocol for custom CRISPR Cas9 donor vector construction to truncate genes in mammalian cells using pcDNA3 backbone (BMC Molecular Biology (2018) 19 (3) DOI: 10.1186/s12867-018-0105-8)",
abstract = "The original article [1] contains three erroneous mentions of usage of a restriction enzyme-BstZ17I-in the Methods section as displayed in the following sentences: 1. [⋯] Therefore, FOXO3 gene fragments (PCR products) had pcDNA3 sequences on the ends that corresponded to upstream and downstream sequences of the utilized restriction sites (DraIII for Arm1 and BstZ17I for Arm2) in pcDNA3. 2. [⋯] The intermediate vector (with FOXO3 Arm 1) was cut with BstZ17I, which is on the other side of the neomycin resistance gene in the pcDNA3 plasmid compared to FOXO3 Arm 1. 3. [⋯] FOXO3 Arm 2 was amplified with the primer pair specified in Table 1, producing a product that had sequences on each end that were identical to the sequences proximal to the BstZ17I site in the intermediate FOXO3 Arm 1 vector. As such, the above three sentences should instead have stated the correct restriction enzyme-BsmI-in place of where BstZ17I was mentioned in each instance.",
author = "Neftali Vazquez and Lilia Sanchez and Rebecca Marks and Eduardo Martinez and Victor Fanniel and Alma Lopez and Andrea Salinas and Itzel Flores and Jesse Hirschmann and Robert Gilkerson and Erin Schuenzel and Robert Dearth and Reginald Halaby and Wendy Innis-Whitehouse and Megan Keniry",
year = "2019",
month = "8",
day = "14",
doi = "10.1186/s12867-019-0138-7",
language = "English",
volume = "20",
journal = "BMC Molecular Biology",
issn = "1471-2199",
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number = "1",

}

Vazquez, N, Sanchez, L, Marks, R, Martinez, E, Fanniel, V, Lopez, A, Salinas, A, Flores, I, Hirschmann, J, Gilkerson, R, Schuenzel, E, Dearth, R, Halaby, R, Innis-Whitehouse, W & Keniry, M 2019, 'Correction to: A protocol for custom CRISPR Cas9 donor vector construction to truncate genes in mammalian cells using pcDNA3 backbone (BMC Molecular Biology (2018) 19 (3) DOI: 10.1186/s12867-018-0105-8)', BMC Molecular Biology, vol. 20, no. 1, 20. https://doi.org/10.1186/s12867-019-0138-7

Correction to : A protocol for custom CRISPR Cas9 donor vector construction to truncate genes in mammalian cells using pcDNA3 backbone (BMC Molecular Biology (2018) 19 (3) DOI: 10.1186/s12867-018-0105-8). / Vazquez, Neftali; Sanchez, Lilia; Marks, Rebecca; Martinez, Eduardo; Fanniel, Victor; Lopez, Alma; Salinas, Andrea; Flores, Itzel; Hirschmann, Jesse; Gilkerson, Robert; Schuenzel, Erin; Dearth, Robert; Halaby, Reginald; Innis-Whitehouse, Wendy; Keniry, Megan.

In: BMC Molecular Biology, Vol. 20, No. 1, 20, 14.08.2019.

Research output: Contribution to journalComment/debateResearch

TY - JOUR

T1 - Correction to

T2 - A protocol for custom CRISPR Cas9 donor vector construction to truncate genes in mammalian cells using pcDNA3 backbone (BMC Molecular Biology (2018) 19 (3) DOI: 10.1186/s12867-018-0105-8)

AU - Vazquez, Neftali

AU - Sanchez, Lilia

AU - Marks, Rebecca

AU - Martinez, Eduardo

AU - Fanniel, Victor

AU - Lopez, Alma

AU - Salinas, Andrea

AU - Flores, Itzel

AU - Hirschmann, Jesse

AU - Gilkerson, Robert

AU - Schuenzel, Erin

AU - Dearth, Robert

AU - Halaby, Reginald

AU - Innis-Whitehouse, Wendy

AU - Keniry, Megan

PY - 2019/8/14

Y1 - 2019/8/14

N2 - The original article [1] contains three erroneous mentions of usage of a restriction enzyme-BstZ17I-in the Methods section as displayed in the following sentences: 1. [⋯] Therefore, FOXO3 gene fragments (PCR products) had pcDNA3 sequences on the ends that corresponded to upstream and downstream sequences of the utilized restriction sites (DraIII for Arm1 and BstZ17I for Arm2) in pcDNA3. 2. [⋯] The intermediate vector (with FOXO3 Arm 1) was cut with BstZ17I, which is on the other side of the neomycin resistance gene in the pcDNA3 plasmid compared to FOXO3 Arm 1. 3. [⋯] FOXO3 Arm 2 was amplified with the primer pair specified in Table 1, producing a product that had sequences on each end that were identical to the sequences proximal to the BstZ17I site in the intermediate FOXO3 Arm 1 vector. As such, the above three sentences should instead have stated the correct restriction enzyme-BsmI-in place of where BstZ17I was mentioned in each instance.

AB - The original article [1] contains three erroneous mentions of usage of a restriction enzyme-BstZ17I-in the Methods section as displayed in the following sentences: 1. [⋯] Therefore, FOXO3 gene fragments (PCR products) had pcDNA3 sequences on the ends that corresponded to upstream and downstream sequences of the utilized restriction sites (DraIII for Arm1 and BstZ17I for Arm2) in pcDNA3. 2. [⋯] The intermediate vector (with FOXO3 Arm 1) was cut with BstZ17I, which is on the other side of the neomycin resistance gene in the pcDNA3 plasmid compared to FOXO3 Arm 1. 3. [⋯] FOXO3 Arm 2 was amplified with the primer pair specified in Table 1, producing a product that had sequences on each end that were identical to the sequences proximal to the BstZ17I site in the intermediate FOXO3 Arm 1 vector. As such, the above three sentences should instead have stated the correct restriction enzyme-BsmI-in place of where BstZ17I was mentioned in each instance.

UR - http://www.scopus.com/inward/record.url?scp=85070973790&partnerID=8YFLogxK

U2 - 10.1186/s12867-019-0138-7

DO - 10.1186/s12867-019-0138-7

M3 - Comment/debate

VL - 20

JO - BMC Molecular Biology

JF - BMC Molecular Biology

SN - 1471-2199

IS - 1

M1 - 20

ER -