Coxsackievirus and adenovirus receptor (CAR) mediates trafficking of acid sensing ion channel 3 (ASIC3) via PSD-95

Katherine J.D.A. Excoffon, Abimbola O. Kolawole, Nobuyoshi Kusama, Nicholas D. Gansemer, Priyanka Sharma, Alesia M. Hruska-Hageman, Elena Petroff, Christopher J. Benson

Research output: Contribution to journalArticlepeer-review

7 Scopus citations


We have previously shown that the Coxsackievirus and adenovirus receptor (CAR) can interact with post-synaptic density 95 (PSD-95) and localize PSD-95 to cell-cell junctions. We have also shown that activity of the acid sensing ion channel (ASIC3), a H+-gated cation channel that plays a role in mechanosensation and pain signaling, is negatively modulated by PSD-95 through a PDZ-based interaction. We asked whether CAR and ASIC3 simultaneously interact with PSD-95, and if so, whether co-expression of these proteins alters their cellular distribution and localization. Results indicate that CAR and ASIC3 co-immunoprecipitate only when co-expressed with PSD-95. CAR also brings both PSD-95 and ASIC3 to the junctions of heterologous cells. Moreover, CAR rescues PSD-95-mediated inhibition of ASIC3 currents. These data suggest that, in addition to activity as a viral receptor and adhesion molecule, CAR can play a role in trafficking proteins, including ion channels, in a PDZ-based scaffolding complex.

Original languageEnglish
Pages (from-to)13-18
Number of pages6
JournalBiochemical and Biophysical Research Communications
Issue number1
StatePublished - 17 Aug 2012


  • ASIC3
  • Coxsackievirus and adenovirus receptor
  • PSD-95
  • Protein trafficking


Dive into the research topics of 'Coxsackievirus and adenovirus receptor (CAR) mediates trafficking of acid sensing ion channel 3 (ASIC3) via PSD-95'. Together they form a unique fingerprint.

Cite this