Coxsackievirus and adenovirus receptor (CAR) mediates trafficking of acid sensing ion channel 3 (ASIC3) via PSD-95

Katherine J.D.A. Excoffon, Abimbola O. Kolawole, Nobuyoshi Kusama, Nicholas D. Gansemer, Priyanka Sharma, Alesia M. Hruska-Hageman, Elena Petroff, Christopher J. Benson

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Abstract

We have previously shown that the Coxsackievirus and adenovirus receptor (CAR) can interact with post-synaptic density 95 (PSD-95) and localize PSD-95 to cell-cell junctions. We have also shown that activity of the acid sensing ion channel (ASIC3), a H+-gated cation channel that plays a role in mechanosensation and pain signaling, is negatively modulated by PSD-95 through a PDZ-based interaction. We asked whether CAR and ASIC3 simultaneously interact with PSD-95, and if so, whether co-expression of these proteins alters their cellular distribution and localization. Results indicate that CAR and ASIC3 co-immunoprecipitate only when co-expressed with PSD-95. CAR also brings both PSD-95 and ASIC3 to the junctions of heterologous cells. Moreover, CAR rescues PSD-95-mediated inhibition of ASIC3 currents. These data suggest that, in addition to activity as a viral receptor and adhesion molecule, CAR can play a role in trafficking proteins, including ion channels, in a PDZ-based scaffolding complex.

Original languageEnglish
Pages (from-to)13-18
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume425
Issue number1
DOIs
StatePublished - 17 Aug 2012

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Acid Sensing Ion Channels
Post-Synaptic Density
Enterovirus
Intercellular Junctions
Ion Channels
Protein Transport
adenovirus receptor
Cations
Proteins
Molecules
Pain

Keywords

  • ASIC3
  • Coxsackievirus and adenovirus receptor
  • PSD-95
  • Protein trafficking

Cite this

Excoffon, K. J. D. A., Kolawole, A. O., Kusama, N., Gansemer, N. D., Sharma, P., Hruska-Hageman, A. M., ... Benson, C. J. (2012). Coxsackievirus and adenovirus receptor (CAR) mediates trafficking of acid sensing ion channel 3 (ASIC3) via PSD-95. Biochemical and Biophysical Research Communications, 425(1), 13-18. https://doi.org/10.1016/j.bbrc.2012.07.033
Excoffon, Katherine J.D.A. ; Kolawole, Abimbola O. ; Kusama, Nobuyoshi ; Gansemer, Nicholas D. ; Sharma, Priyanka ; Hruska-Hageman, Alesia M. ; Petroff, Elena ; Benson, Christopher J. / Coxsackievirus and adenovirus receptor (CAR) mediates trafficking of acid sensing ion channel 3 (ASIC3) via PSD-95. In: Biochemical and Biophysical Research Communications. 2012 ; Vol. 425, No. 1. pp. 13-18.
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Coxsackievirus and adenovirus receptor (CAR) mediates trafficking of acid sensing ion channel 3 (ASIC3) via PSD-95. / Excoffon, Katherine J.D.A.; Kolawole, Abimbola O.; Kusama, Nobuyoshi; Gansemer, Nicholas D.; Sharma, Priyanka; Hruska-Hageman, Alesia M.; Petroff, Elena; Benson, Christopher J.

In: Biochemical and Biophysical Research Communications, Vol. 425, No. 1, 17.08.2012, p. 13-18.

Research output: Contribution to journalArticleResearchpeer-review

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AU - Excoffon, Katherine J.D.A.

AU - Kolawole, Abimbola O.

AU - Kusama, Nobuyoshi

AU - Gansemer, Nicholas D.

AU - Sharma, Priyanka

AU - Hruska-Hageman, Alesia M.

AU - Petroff, Elena

AU - Benson, Christopher J.

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