TY - JOUR
T1 - Crystallization and initial X-ray analysis of rabbit mature sterol carrier protein 2
AU - Choinowski, Thomas
AU - Dyer, James H.
AU - Maderegger, Bernhard
AU - Winterhalter, Kaspar H.
AU - Hauser, Helmut
AU - Piontek, Klaus
PY - 1999/8
Y1 - 1999/8
N2 - Sterol carrier protein 2 (SCP2) is a basic intracellular protein which facilitates the in vitro intermembrane transfer of cholesterol, phospholipids and glycolipids. SCP2 was expressed in Escherichia coli, purified to apparent electrophoretic homogeneity and crystallized. Single crystals were obtained by hanging-drop vapour diffusion using ammonium sulfate as precipitant. These crystals belong to space group P41212 or its enantiomorph, with unit-cell parameters a = b = 57.5, c = 86.5 Å, and have one molecule in the crystallographic asymmetric unit. Intensity data to 1.8 Å resolution were collected from native SCP2 crystals using synchrotron radiation, were processed and scaled with an R(linear) = 4.9%.
AB - Sterol carrier protein 2 (SCP2) is a basic intracellular protein which facilitates the in vitro intermembrane transfer of cholesterol, phospholipids and glycolipids. SCP2 was expressed in Escherichia coli, purified to apparent electrophoretic homogeneity and crystallized. Single crystals were obtained by hanging-drop vapour diffusion using ammonium sulfate as precipitant. These crystals belong to space group P41212 or its enantiomorph, with unit-cell parameters a = b = 57.5, c = 86.5 Å, and have one molecule in the crystallographic asymmetric unit. Intensity data to 1.8 Å resolution were collected from native SCP2 crystals using synchrotron radiation, were processed and scaled with an R(linear) = 4.9%.
UR - http://www.scopus.com/inward/record.url?scp=0033179781&partnerID=8YFLogxK
U2 - 10.1107/S0907444999007106
DO - 10.1107/S0907444999007106
M3 - Article
C2 - 10417421
AN - SCOPUS:0033179781
SN - 0907-4449
VL - 55
SP - 1478
EP - 1480
JO - Acta Crystallographica Section D: Biological Crystallography
JF - Acta Crystallographica Section D: Biological Crystallography
IS - 8
ER -