Effect of pharmacological lowering of plasma urate on exercise-induced oxidative stress

S. R. McAnulty, Peter Hosick, L. S. McAnulty, J. C. Quindry, L. Still, M. B. Hudson, A. N. Dibarnardi, G. L. Milne, J. D. Morrow, M. D. Austin

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Abstract

Urate is a metabolic end product of purine metabolism that contributes about 66% of the antioxidant capacity of plasma. The objective of this study was to evaluate the importance of plasma urate as an antioxidant using pharmacological lowering and examining the impact on plasma antioxidant capacity and oxidative stress after intense exercise. Fifteen subjects ran for 45 min at ∼80% VO2 max under the influence of probenecid (1 g/d) (PRO) or placebo (PLA) in a doubleblind, crossover design. Blood samples obtained at baseline, pre-exercise, and immediately post-exercise were analyzed for F2-isoprostanes, lipid hydroperoxides (LHs), ferric-reducing ability of plasma (FRAP), urate, ascorbate (AA), and nitrite. A 2 (group) x 2 (time) repeated-measures analysis of variance (ANOVA), one-way ANOVA, Tukey-Kramer multiple comparison tests, and Student's t tests were used for statistical analysis. PRO exhibited lowered urate and FRAP compared with baseline (p ≤ 0.05), and group effects existed for the exercise trials (p = 0.023 and p ≤ 0.001, respectively) versus PLA. F2-isoprostanes, nitrite, and AA were increased after exercise (p = 0.004, p = 0.001, and p = 0.003, respectively), but the pattern of change was not different between treatments. This study indicates that plasma markers of exercise-induced oxidative stress were not affected by below-normal physiological concentrations of urate and a diminished antioxidant capacity within the plasma compartment.

Original languageEnglish
Pages (from-to)1148-1155
Number of pages8
JournalApplied Physiology, Nutrition and Metabolism
Volume32
Issue number6
DOIs
StatePublished - 1 Dec 2007

Fingerprint

Uric Acid
Oxidative Stress
Pharmacology
Exercise
Antioxidants
F2-Isoprostanes
Nitrites
Analysis of Variance
Placebos
Probenecid
Lipid Peroxides
Cross-Over Studies
Students

Keywords

  • Exercise
  • F2-isoprostanes
  • Ferric reducing ability of plasma
  • Oxidative stress
  • Probenecid
  • Urate

Cite this

McAnulty, S. R., Hosick, P., McAnulty, L. S., Quindry, J. C., Still, L., Hudson, M. B., ... Austin, M. D. (2007). Effect of pharmacological lowering of plasma urate on exercise-induced oxidative stress. Applied Physiology, Nutrition and Metabolism, 32(6), 1148-1155. https://doi.org/10.1139/H07-131
McAnulty, S. R. ; Hosick, Peter ; McAnulty, L. S. ; Quindry, J. C. ; Still, L. ; Hudson, M. B. ; Dibarnardi, A. N. ; Milne, G. L. ; Morrow, J. D. ; Austin, M. D. / Effect of pharmacological lowering of plasma urate on exercise-induced oxidative stress. In: Applied Physiology, Nutrition and Metabolism. 2007 ; Vol. 32, No. 6. pp. 1148-1155.
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McAnulty, SR, Hosick, P, McAnulty, LS, Quindry, JC, Still, L, Hudson, MB, Dibarnardi, AN, Milne, GL, Morrow, JD & Austin, MD 2007, 'Effect of pharmacological lowering of plasma urate on exercise-induced oxidative stress', Applied Physiology, Nutrition and Metabolism, vol. 32, no. 6, pp. 1148-1155. https://doi.org/10.1139/H07-131

Effect of pharmacological lowering of plasma urate on exercise-induced oxidative stress. / McAnulty, S. R.; Hosick, Peter; McAnulty, L. S.; Quindry, J. C.; Still, L.; Hudson, M. B.; Dibarnardi, A. N.; Milne, G. L.; Morrow, J. D.; Austin, M. D.

In: Applied Physiology, Nutrition and Metabolism, Vol. 32, No. 6, 01.12.2007, p. 1148-1155.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Effect of pharmacological lowering of plasma urate on exercise-induced oxidative stress

AU - McAnulty, S. R.

AU - Hosick, Peter

AU - McAnulty, L. S.

AU - Quindry, J. C.

AU - Still, L.

AU - Hudson, M. B.

AU - Dibarnardi, A. N.

AU - Milne, G. L.

AU - Morrow, J. D.

AU - Austin, M. D.

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N2 - Urate is a metabolic end product of purine metabolism that contributes about 66% of the antioxidant capacity of plasma. The objective of this study was to evaluate the importance of plasma urate as an antioxidant using pharmacological lowering and examining the impact on plasma antioxidant capacity and oxidative stress after intense exercise. Fifteen subjects ran for 45 min at ∼80% VO2 max under the influence of probenecid (1 g/d) (PRO) or placebo (PLA) in a doubleblind, crossover design. Blood samples obtained at baseline, pre-exercise, and immediately post-exercise were analyzed for F2-isoprostanes, lipid hydroperoxides (LHs), ferric-reducing ability of plasma (FRAP), urate, ascorbate (AA), and nitrite. A 2 (group) x 2 (time) repeated-measures analysis of variance (ANOVA), one-way ANOVA, Tukey-Kramer multiple comparison tests, and Student's t tests were used for statistical analysis. PRO exhibited lowered urate and FRAP compared with baseline (p ≤ 0.05), and group effects existed for the exercise trials (p = 0.023 and p ≤ 0.001, respectively) versus PLA. F2-isoprostanes, nitrite, and AA were increased after exercise (p = 0.004, p = 0.001, and p = 0.003, respectively), but the pattern of change was not different between treatments. This study indicates that plasma markers of exercise-induced oxidative stress were not affected by below-normal physiological concentrations of urate and a diminished antioxidant capacity within the plasma compartment.

AB - Urate is a metabolic end product of purine metabolism that contributes about 66% of the antioxidant capacity of plasma. The objective of this study was to evaluate the importance of plasma urate as an antioxidant using pharmacological lowering and examining the impact on plasma antioxidant capacity and oxidative stress after intense exercise. Fifteen subjects ran for 45 min at ∼80% VO2 max under the influence of probenecid (1 g/d) (PRO) or placebo (PLA) in a doubleblind, crossover design. Blood samples obtained at baseline, pre-exercise, and immediately post-exercise were analyzed for F2-isoprostanes, lipid hydroperoxides (LHs), ferric-reducing ability of plasma (FRAP), urate, ascorbate (AA), and nitrite. A 2 (group) x 2 (time) repeated-measures analysis of variance (ANOVA), one-way ANOVA, Tukey-Kramer multiple comparison tests, and Student's t tests were used for statistical analysis. PRO exhibited lowered urate and FRAP compared with baseline (p ≤ 0.05), and group effects existed for the exercise trials (p = 0.023 and p ≤ 0.001, respectively) versus PLA. F2-isoprostanes, nitrite, and AA were increased after exercise (p = 0.004, p = 0.001, and p = 0.003, respectively), but the pattern of change was not different between treatments. This study indicates that plasma markers of exercise-induced oxidative stress were not affected by below-normal physiological concentrations of urate and a diminished antioxidant capacity within the plasma compartment.

KW - Exercise

KW - F2-isoprostanes

KW - Ferric reducing ability of plasma

KW - Oxidative stress

KW - Probenecid

KW - Urate

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DO - 10.1139/H07-131

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