Electrospinning fabrication, structural and mechanical characterization of rod-like virus-based composite nanofibers

Laying Wu, Jianfeng Zang, L. Andrew Lee, Zhongwei Niu, Gary C. Horvatha, Vaughn Braxtona, Arief Cahyo Wibowo, Michael A. Bruckman, Soumitra Ghoshroy, Hans Conrad Zur Loye, Xiaodong Li, Qian Wang

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Abstract

Tobacco mosaic virus (TMV) was electrospun with polyvinyl alcohol (PVA) into continuous TMV-PVA composite nanofibers to form a biodegradable nonwoven fibrous mat as an extracellular matrix (ECM) mimetic. Morphological characterizations by electron microscopy showed that the addition of varying amounts of TMV resulted in homogeneous nanofibers without phase separation and did not change the diameter of the composite nanofibers. The orientation of TMV in as-spun fibers could be readily controlled and post-processing of the nonwoven TMV-PVA mat significantly improved its water resistance. In addition, tensile tests were performed on individual nanofibers, which revealed that the TMV-PVA composite nanofibers achieved a comparable Young's modulus as PVA nanofibers. Since the modification of TMV is readily achieved via genetic or chemical methods, this process offers a facile way to incorporate a variety of functionalities into polymer nanofibers. As a demonstration of its potential as ECM mimetic, a mutant TMV containing RGD peptide was co-spun with PVA and the resulting fibrous substrates were used to promote cell growth.

Original languageEnglish
Pages (from-to)8550-8557
Number of pages8
JournalJournal of Materials Chemistry
Volume21
Issue number24
DOIs
StatePublished - 28 Jun 2011

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Tobacco
Electrospinning
Nanofibers
Viruses
Polyvinyl Alcohol
Polyvinyl alcohols
Fabrication
Composite materials
Cell growth
Phase separation
Peptides
Electron microscopy
Polymers
Demonstrations
Elastic moduli
Water
Fibers
Substrates
Processing

Cite this

Wu, Laying ; Zang, Jianfeng ; Lee, L. Andrew ; Niu, Zhongwei ; Horvatha, Gary C. ; Braxtona, Vaughn ; Wibowo, Arief Cahyo ; Bruckman, Michael A. ; Ghoshroy, Soumitra ; Zur Loye, Hans Conrad ; Li, Xiaodong ; Wang, Qian. / Electrospinning fabrication, structural and mechanical characterization of rod-like virus-based composite nanofibers. In: Journal of Materials Chemistry. 2011 ; Vol. 21, No. 24. pp. 8550-8557.
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abstract = "Tobacco mosaic virus (TMV) was electrospun with polyvinyl alcohol (PVA) into continuous TMV-PVA composite nanofibers to form a biodegradable nonwoven fibrous mat as an extracellular matrix (ECM) mimetic. Morphological characterizations by electron microscopy showed that the addition of varying amounts of TMV resulted in homogeneous nanofibers without phase separation and did not change the diameter of the composite nanofibers. The orientation of TMV in as-spun fibers could be readily controlled and post-processing of the nonwoven TMV-PVA mat significantly improved its water resistance. In addition, tensile tests were performed on individual nanofibers, which revealed that the TMV-PVA composite nanofibers achieved a comparable Young's modulus as PVA nanofibers. Since the modification of TMV is readily achieved via genetic or chemical methods, this process offers a facile way to incorporate a variety of functionalities into polymer nanofibers. As a demonstration of its potential as ECM mimetic, a mutant TMV containing RGD peptide was co-spun with PVA and the resulting fibrous substrates were used to promote cell growth.",
author = "Laying Wu and Jianfeng Zang and Lee, {L. Andrew} and Zhongwei Niu and Horvatha, {Gary C.} and Vaughn Braxtona and Wibowo, {Arief Cahyo} and Bruckman, {Michael A.} and Soumitra Ghoshroy and {Zur Loye}, {Hans Conrad} and Xiaodong Li and Qian Wang",
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Wu, L, Zang, J, Lee, LA, Niu, Z, Horvatha, GC, Braxtona, V, Wibowo, AC, Bruckman, MA, Ghoshroy, S, Zur Loye, HC, Li, X & Wang, Q 2011, 'Electrospinning fabrication, structural and mechanical characterization of rod-like virus-based composite nanofibers', Journal of Materials Chemistry, vol. 21, no. 24, pp. 8550-8557. https://doi.org/10.1039/c1jm00078k

Electrospinning fabrication, structural and mechanical characterization of rod-like virus-based composite nanofibers. / Wu, Laying; Zang, Jianfeng; Lee, L. Andrew; Niu, Zhongwei; Horvatha, Gary C.; Braxtona, Vaughn; Wibowo, Arief Cahyo; Bruckman, Michael A.; Ghoshroy, Soumitra; Zur Loye, Hans Conrad; Li, Xiaodong; Wang, Qian.

In: Journal of Materials Chemistry, Vol. 21, No. 24, 28.06.2011, p. 8550-8557.

Research output: Contribution to journalArticleResearchpeer-review

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T1 - Electrospinning fabrication, structural and mechanical characterization of rod-like virus-based composite nanofibers

AU - Wu, Laying

AU - Zang, Jianfeng

AU - Lee, L. Andrew

AU - Niu, Zhongwei

AU - Horvatha, Gary C.

AU - Braxtona, Vaughn

AU - Wibowo, Arief Cahyo

AU - Bruckman, Michael A.

AU - Ghoshroy, Soumitra

AU - Zur Loye, Hans Conrad

AU - Li, Xiaodong

AU - Wang, Qian

PY - 2011/6/28

Y1 - 2011/6/28

N2 - Tobacco mosaic virus (TMV) was electrospun with polyvinyl alcohol (PVA) into continuous TMV-PVA composite nanofibers to form a biodegradable nonwoven fibrous mat as an extracellular matrix (ECM) mimetic. Morphological characterizations by electron microscopy showed that the addition of varying amounts of TMV resulted in homogeneous nanofibers without phase separation and did not change the diameter of the composite nanofibers. The orientation of TMV in as-spun fibers could be readily controlled and post-processing of the nonwoven TMV-PVA mat significantly improved its water resistance. In addition, tensile tests were performed on individual nanofibers, which revealed that the TMV-PVA composite nanofibers achieved a comparable Young's modulus as PVA nanofibers. Since the modification of TMV is readily achieved via genetic or chemical methods, this process offers a facile way to incorporate a variety of functionalities into polymer nanofibers. As a demonstration of its potential as ECM mimetic, a mutant TMV containing RGD peptide was co-spun with PVA and the resulting fibrous substrates were used to promote cell growth.

AB - Tobacco mosaic virus (TMV) was electrospun with polyvinyl alcohol (PVA) into continuous TMV-PVA composite nanofibers to form a biodegradable nonwoven fibrous mat as an extracellular matrix (ECM) mimetic. Morphological characterizations by electron microscopy showed that the addition of varying amounts of TMV resulted in homogeneous nanofibers without phase separation and did not change the diameter of the composite nanofibers. The orientation of TMV in as-spun fibers could be readily controlled and post-processing of the nonwoven TMV-PVA mat significantly improved its water resistance. In addition, tensile tests were performed on individual nanofibers, which revealed that the TMV-PVA composite nanofibers achieved a comparable Young's modulus as PVA nanofibers. Since the modification of TMV is readily achieved via genetic or chemical methods, this process offers a facile way to incorporate a variety of functionalities into polymer nanofibers. As a demonstration of its potential as ECM mimetic, a mutant TMV containing RGD peptide was co-spun with PVA and the resulting fibrous substrates were used to promote cell growth.

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DO - 10.1039/c1jm00078k

M3 - Article

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JO - Journal of Materials Chemistry

JF - Journal of Materials Chemistry

SN - 0959-9428

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