Electrospun fibrous scaffolds promote breast cancer cell alignment and epithelial-mesenchymal transition

Sharmistha Saha, Xinrui Duan, Laying Wu, Pang Kuo Lo, Hexin Chen, Qian Wang

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

In this work we created electrospun fibrous scaffolds with random and aligned fiber orientations in order to mimic the three-dimensional structure of the natural extracellular matrix (ECM). The rigidity and topography of the ECM environment have been reported to alter cancer cell behavior. However, the complexity of the in vivo system makes it difficult to isolate and study such extracellular topographical cues that trigger cancer cells' response. Breast cancer cells were cultured on these fibrous scaffolds for 3-5 days. The cells showed elongated spindle-like morphology in the aligned fibers, whereas they maintained a mostly flat stellar shape in the random fibers. Gene expression profiling of these cells post seeding showed up-regulation of transforming growth factor β-1 (TGFβ-1) along with other mesenchymal biomarkers, suggesting that these cells undergo epithelial-mesenchymal transitions in response to the polymer scaffold. The results of this study indicate that the topographical cue may play a significant role in tumor progression.

Original languageEnglish
Pages (from-to)2028-2034
Number of pages7
JournalLangmuir
Volume28
Issue number4
DOIs
StatePublished - 31 Jan 2012

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Scaffolds
breast
cancer
alignment
Cells
cues
cells
fiber orientation
fibers
spindles
Fibers
biomarkers
gene expression
Transforming Growth Factors
Biomarkers
inoculation
Fiber reinforced materials
matrices
cultured cells
rigidity

Cite this

Saha, Sharmistha ; Duan, Xinrui ; Wu, Laying ; Lo, Pang Kuo ; Chen, Hexin ; Wang, Qian. / Electrospun fibrous scaffolds promote breast cancer cell alignment and epithelial-mesenchymal transition. In: Langmuir. 2012 ; Vol. 28, No. 4. pp. 2028-2034.
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Electrospun fibrous scaffolds promote breast cancer cell alignment and epithelial-mesenchymal transition. / Saha, Sharmistha; Duan, Xinrui; Wu, Laying; Lo, Pang Kuo; Chen, Hexin; Wang, Qian.

In: Langmuir, Vol. 28, No. 4, 31.01.2012, p. 2028-2034.

Research output: Contribution to journalArticle

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