Enhancing digestibility and ethanol yield of Populus wood via expression of an engineered monolignol 4-O-methyltransferase

Yuanheng Cai, Kewei Zhang, Hoon Kim, Guichuan Hou, Xuebin Zhang, Huijun Yang, Huan Feng, Lisa Miller, John Ralph, Chang Jun Liu

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Producing cellulosic biofuels and bio-based chemicals from woody biomass is impeded by the presence of lignin polymer in the plant cell wall. Manipulating the monolignol biosynthetic pathway offers a promising approach to improved processability, but often impairs plant growth and development. Here, we show that expressing an engineered 4-O-methyltransferase that chemically modifies the phenolic moiety of lignin monomeric precursors, thus preventing their incorporation into the lignin polymer, substantially alters hybrid aspens' lignin content and structure. Woody biomass derived from the transgenic aspens shows a 62% increase in the release of simple sugars and up to a 49% increase in the yield of ethanol when the woody biomass is subjected to enzymatic digestion and yeast-mediated fermentation. Moreover, the cell wall structural changes do not affect growth and biomass production of the trees. Our study provides a useful strategy for tailoring woody biomass for bio-based applications.

Original languageEnglish
Article number11989
JournalNature Communications
Volume7
DOIs
StatePublished - 28 Jun 2016

Fingerprint

Populus
Methyltransferases
biomass
lignin
Biomass
Lignin
Wood
ethyl alcohol
Ethanol
Cell Wall
Polymers
fermentation
Plant Development
Biofuels
Biosynthetic Pathways
yeast
Plant Cells
polymers
sugars
Growth and Development

Cite this

Cai, Yuanheng ; Zhang, Kewei ; Kim, Hoon ; Hou, Guichuan ; Zhang, Xuebin ; Yang, Huijun ; Feng, Huan ; Miller, Lisa ; Ralph, John ; Liu, Chang Jun. / Enhancing digestibility and ethanol yield of Populus wood via expression of an engineered monolignol 4-O-methyltransferase. In: Nature Communications. 2016 ; Vol. 7.
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abstract = "Producing cellulosic biofuels and bio-based chemicals from woody biomass is impeded by the presence of lignin polymer in the plant cell wall. Manipulating the monolignol biosynthetic pathway offers a promising approach to improved processability, but often impairs plant growth and development. Here, we show that expressing an engineered 4-O-methyltransferase that chemically modifies the phenolic moiety of lignin monomeric precursors, thus preventing their incorporation into the lignin polymer, substantially alters hybrid aspens' lignin content and structure. Woody biomass derived from the transgenic aspens shows a 62{\%} increase in the release of simple sugars and up to a 49{\%} increase in the yield of ethanol when the woody biomass is subjected to enzymatic digestion and yeast-mediated fermentation. Moreover, the cell wall structural changes do not affect growth and biomass production of the trees. Our study provides a useful strategy for tailoring woody biomass for bio-based applications.",
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Enhancing digestibility and ethanol yield of Populus wood via expression of an engineered monolignol 4-O-methyltransferase. / Cai, Yuanheng; Zhang, Kewei; Kim, Hoon; Hou, Guichuan; Zhang, Xuebin; Yang, Huijun; Feng, Huan; Miller, Lisa; Ralph, John; Liu, Chang Jun.

In: Nature Communications, Vol. 7, 11989, 28.06.2016.

Research output: Contribution to journalArticle

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