Ethylene-regulated gene expression

Molecular cloning of the genes encoding an endochitinase from Phaseolus vulgaris

K. E. Broglie, John Gaynor, R. M. Broglie

Research output: Contribution to journalArticleResearchpeer-review

210 Citations (Scopus)

Abstract

A full-length copy of bean leaf chitinase mRNA has been cloned. The 1146-base-pair insert of pCH18 encodes the 27-residue amino-terminal signal peptide of the precursor and 301 residues of the mature protein. Utilizing pCH18 as a hybridization probe, we have shown that the increase in translatable chitinase mRNA seen upon ehylene treatment of bean seedlings is due to a 75- to 100-fold increase in steady-state mRNA levels. Southern blot analysis of bean genomic DNA revealed that chitinase is encoded by a small, multigene family consisting of approximately four members. From our nucleotide sequence analysis of five additional chitinase cDNA clones, it appears that at least two of these genes are expressed. Three of the bean chitinase genes have been isolated from a Sau3A genomic library and partially characterized.

Original languageEnglish
Pages (from-to)6820-6824
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume83
Issue number18
DOIs
StatePublished - 1 Jan 1986

Fingerprint

Chitinases
Phaseolus
Molecular Cloning
Gene Expression
Genes
Messenger RNA
Genomic Library
Multigene Family
Protein Sorting Signals
Southern Blotting
Seedlings
Base Pairing
Sequence Analysis
Complementary DNA
Clone Cells
ethylene
DNA
Proteins

Cite this

@article{bf4c52049c7d418a8987421b574e4433,
title = "Ethylene-regulated gene expression: Molecular cloning of the genes encoding an endochitinase from Phaseolus vulgaris",
abstract = "A full-length copy of bean leaf chitinase mRNA has been cloned. The 1146-base-pair insert of pCH18 encodes the 27-residue amino-terminal signal peptide of the precursor and 301 residues of the mature protein. Utilizing pCH18 as a hybridization probe, we have shown that the increase in translatable chitinase mRNA seen upon ehylene treatment of bean seedlings is due to a 75- to 100-fold increase in steady-state mRNA levels. Southern blot analysis of bean genomic DNA revealed that chitinase is encoded by a small, multigene family consisting of approximately four members. From our nucleotide sequence analysis of five additional chitinase cDNA clones, it appears that at least two of these genes are expressed. Three of the bean chitinase genes have been isolated from a Sau3A genomic library and partially characterized.",
author = "Broglie, {K. E.} and John Gaynor and Broglie, {R. M.}",
year = "1986",
month = "1",
day = "1",
doi = "10.1073/pnas.83.18.6820",
language = "English",
volume = "83",
pages = "6820--6824",
journal = "Proceedings of the National Academy of Sciences of the United States of America",
issn = "0027-8424",
number = "18",

}

Ethylene-regulated gene expression : Molecular cloning of the genes encoding an endochitinase from Phaseolus vulgaris. / Broglie, K. E.; Gaynor, John; Broglie, R. M.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 83, No. 18, 01.01.1986, p. 6820-6824.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Ethylene-regulated gene expression

T2 - Molecular cloning of the genes encoding an endochitinase from Phaseolus vulgaris

AU - Broglie, K. E.

AU - Gaynor, John

AU - Broglie, R. M.

PY - 1986/1/1

Y1 - 1986/1/1

N2 - A full-length copy of bean leaf chitinase mRNA has been cloned. The 1146-base-pair insert of pCH18 encodes the 27-residue amino-terminal signal peptide of the precursor and 301 residues of the mature protein. Utilizing pCH18 as a hybridization probe, we have shown that the increase in translatable chitinase mRNA seen upon ehylene treatment of bean seedlings is due to a 75- to 100-fold increase in steady-state mRNA levels. Southern blot analysis of bean genomic DNA revealed that chitinase is encoded by a small, multigene family consisting of approximately four members. From our nucleotide sequence analysis of five additional chitinase cDNA clones, it appears that at least two of these genes are expressed. Three of the bean chitinase genes have been isolated from a Sau3A genomic library and partially characterized.

AB - A full-length copy of bean leaf chitinase mRNA has been cloned. The 1146-base-pair insert of pCH18 encodes the 27-residue amino-terminal signal peptide of the precursor and 301 residues of the mature protein. Utilizing pCH18 as a hybridization probe, we have shown that the increase in translatable chitinase mRNA seen upon ehylene treatment of bean seedlings is due to a 75- to 100-fold increase in steady-state mRNA levels. Southern blot analysis of bean genomic DNA revealed that chitinase is encoded by a small, multigene family consisting of approximately four members. From our nucleotide sequence analysis of five additional chitinase cDNA clones, it appears that at least two of these genes are expressed. Three of the bean chitinase genes have been isolated from a Sau3A genomic library and partially characterized.

UR - http://www.scopus.com/inward/record.url?scp=0022537615&partnerID=8YFLogxK

U2 - 10.1073/pnas.83.18.6820

DO - 10.1073/pnas.83.18.6820

M3 - Article

VL - 83

SP - 6820

EP - 6824

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

SN - 0027-8424

IS - 18

ER -