Ethylene-regulated gene expression: Molecular cloning of the genes encoding an endochitinase from Phaseolus vulgaris

K. E. Broglie, J. J. Gaynor, R. M. Broglie

Research output: Contribution to journalArticlepeer-review

215 Scopus citations

Abstract

A full-length copy of bean leaf chitinase mRNA has been cloned. The 1146-base-pair insert of pCH18 encodes the 27-residue amino-terminal signal peptide of the precursor and 301 residues of the mature protein. Utilizing pCH18 as a hybridization probe, we have shown that the increase in translatable chitinase mRNA seen upon ehylene treatment of bean seedlings is due to a 75- to 100-fold increase in steady-state mRNA levels. Southern blot analysis of bean genomic DNA revealed that chitinase is encoded by a small, multigene family consisting of approximately four members. From our nucleotide sequence analysis of five additional chitinase cDNA clones, it appears that at least two of these genes are expressed. Three of the bean chitinase genes have been isolated from a Sau3A genomic library and partially characterized.

Original languageEnglish
Pages (from-to)6820-6824
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume83
Issue number18
DOIs
StatePublished - 1 Jan 1986

Fingerprint Dive into the research topics of 'Ethylene-regulated gene expression: Molecular cloning of the genes encoding an endochitinase from Phaseolus vulgaris'. Together they form a unique fingerprint.

Cite this