Evidence for exocellular Arsenic in Fronds of Pteris vittata

Rupali Datta, Padmini Das, Ryan Tappero, Pravin Punamiya, Evert Elzinga, Shivendra Sahi, Huan Feng, Jeffrey Kiiskila, Dibyendu Sarkar

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Abstract

The arsenic (As) hyperaccumulating fern species Pteris vittata (PV) is capable of accumulating large quantities of As in its aboveground tissues. Transformation to AsIII and vacuolar sequestration is believed to be the As detoxification mechanism in PV. Here we present evidence for a preponderance of exocellular As in fronds of Pteris vittata despite numerous reports of a tolerance mechanism involving intracellular compartmentalization. Results of an extraction experiment show that 43-71% of the As extruded out of the fronds of PV grown in 0.67, 3.3 and 6.7 mM AsV. SEM-EDX analysis showed that As was localized largely on the lower pinna surface, with smaller amounts on the upper surface, as crystalline deposits. X-ray fluorescence imaging of pinna cross-sections revealed preferential localization of As on the pinna surface in the proximity of veins, with the majority localized near the midrib. Majority of the As in the pinnae is contained in the apoplast rather than vacuoles. Our results provide evidence that exocellular sequestration is potentially a mechanism of As detoxification in PV, particularly at higher As concentrations, raising concern about its use for phytoremediation.

Original languageEnglish
Article number2839
JournalScientific Reports
Volume7
Issue number1
DOIs
StatePublished - 1 Dec 2017

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arsenic
detoxification
compartmentalization
phytoremediation
fern
X-ray fluorescence
tolerance
cross section
scanning electron microscopy

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Datta, R., Das, P., Tappero, R., Punamiya, P., Elzinga, E., Sahi, S., ... Sarkar, D. (2017). Evidence for exocellular Arsenic in Fronds of Pteris vittata. Scientific Reports, 7(1), [2839]. https://doi.org/10.1038/s41598-017-03194-x
Datta, Rupali ; Das, Padmini ; Tappero, Ryan ; Punamiya, Pravin ; Elzinga, Evert ; Sahi, Shivendra ; Feng, Huan ; Kiiskila, Jeffrey ; Sarkar, Dibyendu. / Evidence for exocellular Arsenic in Fronds of Pteris vittata. In: Scientific Reports. 2017 ; Vol. 7, No. 1.
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abstract = "The arsenic (As) hyperaccumulating fern species Pteris vittata (PV) is capable of accumulating large quantities of As in its aboveground tissues. Transformation to AsIII and vacuolar sequestration is believed to be the As detoxification mechanism in PV. Here we present evidence for a preponderance of exocellular As in fronds of Pteris vittata despite numerous reports of a tolerance mechanism involving intracellular compartmentalization. Results of an extraction experiment show that 43-71{\%} of the As extruded out of the fronds of PV grown in 0.67, 3.3 and 6.7 mM AsV. SEM-EDX analysis showed that As was localized largely on the lower pinna surface, with smaller amounts on the upper surface, as crystalline deposits. X-ray fluorescence imaging of pinna cross-sections revealed preferential localization of As on the pinna surface in the proximity of veins, with the majority localized near the midrib. Majority of the As in the pinnae is contained in the apoplast rather than vacuoles. Our results provide evidence that exocellular sequestration is potentially a mechanism of As detoxification in PV, particularly at higher As concentrations, raising concern about its use for phytoremediation.",
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Datta, R, Das, P, Tappero, R, Punamiya, P, Elzinga, E, Sahi, S, Feng, H, Kiiskila, J & Sarkar, D 2017, 'Evidence for exocellular Arsenic in Fronds of Pteris vittata', Scientific Reports, vol. 7, no. 1, 2839. https://doi.org/10.1038/s41598-017-03194-x

Evidence for exocellular Arsenic in Fronds of Pteris vittata. / Datta, Rupali; Das, Padmini; Tappero, Ryan; Punamiya, Pravin; Elzinga, Evert; Sahi, Shivendra; Feng, Huan; Kiiskila, Jeffrey; Sarkar, Dibyendu.

In: Scientific Reports, Vol. 7, No. 1, 2839, 01.12.2017.

Research output: Contribution to journalArticleResearchpeer-review

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AU - Das, Padmini

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AU - Punamiya, Pravin

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AU - Sahi, Shivendra

AU - Feng, Huan

AU - Kiiskila, Jeffrey

AU - Sarkar, Dibyendu

PY - 2017/12/1

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N2 - The arsenic (As) hyperaccumulating fern species Pteris vittata (PV) is capable of accumulating large quantities of As in its aboveground tissues. Transformation to AsIII and vacuolar sequestration is believed to be the As detoxification mechanism in PV. Here we present evidence for a preponderance of exocellular As in fronds of Pteris vittata despite numerous reports of a tolerance mechanism involving intracellular compartmentalization. Results of an extraction experiment show that 43-71% of the As extruded out of the fronds of PV grown in 0.67, 3.3 and 6.7 mM AsV. SEM-EDX analysis showed that As was localized largely on the lower pinna surface, with smaller amounts on the upper surface, as crystalline deposits. X-ray fluorescence imaging of pinna cross-sections revealed preferential localization of As on the pinna surface in the proximity of veins, with the majority localized near the midrib. Majority of the As in the pinnae is contained in the apoplast rather than vacuoles. Our results provide evidence that exocellular sequestration is potentially a mechanism of As detoxification in PV, particularly at higher As concentrations, raising concern about its use for phytoremediation.

AB - The arsenic (As) hyperaccumulating fern species Pteris vittata (PV) is capable of accumulating large quantities of As in its aboveground tissues. Transformation to AsIII and vacuolar sequestration is believed to be the As detoxification mechanism in PV. Here we present evidence for a preponderance of exocellular As in fronds of Pteris vittata despite numerous reports of a tolerance mechanism involving intracellular compartmentalization. Results of an extraction experiment show that 43-71% of the As extruded out of the fronds of PV grown in 0.67, 3.3 and 6.7 mM AsV. SEM-EDX analysis showed that As was localized largely on the lower pinna surface, with smaller amounts on the upper surface, as crystalline deposits. X-ray fluorescence imaging of pinna cross-sections revealed preferential localization of As on the pinna surface in the proximity of veins, with the majority localized near the midrib. Majority of the As in the pinnae is contained in the apoplast rather than vacuoles. Our results provide evidence that exocellular sequestration is potentially a mechanism of As detoxification in PV, particularly at higher As concentrations, raising concern about its use for phytoremediation.

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Datta R, Das P, Tappero R, Punamiya P, Elzinga E, Sahi S et al. Evidence for exocellular Arsenic in Fronds of Pteris vittata. Scientific Reports. 2017 Dec 1;7(1). 2839. https://doi.org/10.1038/s41598-017-03194-x