Evidence for the Early Evolutionary Loss of the M20D Auxin Amidohydrolase Family from Mosses and Horizontal Gene Transfer from Soil Bacteria of Cryptic Hydrolase Orthologues to Physcomitrella patens

James Campanella, Stephanie Kurdach, Richard Skibitski, John V. Smalley, Samuel Desind, Jutta Ludwig-Müller

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Inactive auxin conjugates are accumulated in plants and hydrolyzed to recover phytohormone action. A family of metallopeptidase orthologues has been conserved in Plantae to help regulate auxin homeostatic levels during growth and development. This hydrolase family was recently traced back to liverwort, the most ancient extant land plant lineage. Liverwort’s auxin hydrolase has little activity against auxin conjugate substrates and does not appear to actively regulate auxin. This finding, along with data that shows moss can synthesize auxin conjugates, led to examining another bryophyte lineage, Physcomitrella patens. We have identified and isolated three M20D hydrolase paralogues from moss. The isolated enzymes strongly recognize and cleave a variety of auxin conjugates, including those of indole butyric and indole propionic acids. These P. patens hydrolases not only appear to be “cryptic”, but they are likely to have derived from soil bacteria through Horizontal Gene Transfer. Additionally, support is presented that the plant-type M20D peptidase family may have been universally lost from mosses after divergence from the common ancestor with liverwort.

Original languageEnglish
JournalJournal of Plant Growth Regulation
DOIs
StatePublished - 1 Jan 2019

Fingerprint

amide hydrolases
Physcomitrella patens
soil bacteria
hydrolases
mosses and liverworts
auxins
indoles
peptidases
embryophytes
metalloproteinases
propionic acid
Plantae
horizontal gene transfer
plant hormones
growth and development
ancestry
enzymes

Keywords

  • Amidohydrolase
  • Auxin conjugate regulation
  • Cryptic genes
  • Horizontal gene transfer
  • Moss evolution
  • Physcomitrella patens

Cite this

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title = "Evidence for the Early Evolutionary Loss of the M20D Auxin Amidohydrolase Family from Mosses and Horizontal Gene Transfer from Soil Bacteria of Cryptic Hydrolase Orthologues to Physcomitrella patens",
abstract = "Inactive auxin conjugates are accumulated in plants and hydrolyzed to recover phytohormone action. A family of metallopeptidase orthologues has been conserved in Plantae to help regulate auxin homeostatic levels during growth and development. This hydrolase family was recently traced back to liverwort, the most ancient extant land plant lineage. Liverwort’s auxin hydrolase has little activity against auxin conjugate substrates and does not appear to actively regulate auxin. This finding, along with data that shows moss can synthesize auxin conjugates, led to examining another bryophyte lineage, Physcomitrella patens. We have identified and isolated three M20D hydrolase paralogues from moss. The isolated enzymes strongly recognize and cleave a variety of auxin conjugates, including those of indole butyric and indole propionic acids. These P. patens hydrolases not only appear to be “cryptic”, but they are likely to have derived from soil bacteria through Horizontal Gene Transfer. Additionally, support is presented that the plant-type M20D peptidase family may have been universally lost from mosses after divergence from the common ancestor with liverwort.",
keywords = "Amidohydrolase, Auxin conjugate regulation, Cryptic genes, Horizontal gene transfer, Moss evolution, Physcomitrella patens",
author = "James Campanella and Stephanie Kurdach and Richard Skibitski and Smalley, {John V.} and Samuel Desind and Jutta Ludwig-M{\"u}ller",
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Evidence for the Early Evolutionary Loss of the M20D Auxin Amidohydrolase Family from Mosses and Horizontal Gene Transfer from Soil Bacteria of Cryptic Hydrolase Orthologues to Physcomitrella patens. / Campanella, James; Kurdach, Stephanie; Skibitski, Richard; Smalley, John V.; Desind, Samuel; Ludwig-Müller, Jutta.

In: Journal of Plant Growth Regulation, 01.01.2019.

Research output: Contribution to journalArticleResearchpeer-review

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AU - Campanella, James

AU - Kurdach, Stephanie

AU - Skibitski, Richard

AU - Smalley, John V.

AU - Desind, Samuel

AU - Ludwig-Müller, Jutta

PY - 2019/1/1

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N2 - Inactive auxin conjugates are accumulated in plants and hydrolyzed to recover phytohormone action. A family of metallopeptidase orthologues has been conserved in Plantae to help regulate auxin homeostatic levels during growth and development. This hydrolase family was recently traced back to liverwort, the most ancient extant land plant lineage. Liverwort’s auxin hydrolase has little activity against auxin conjugate substrates and does not appear to actively regulate auxin. This finding, along with data that shows moss can synthesize auxin conjugates, led to examining another bryophyte lineage, Physcomitrella patens. We have identified and isolated three M20D hydrolase paralogues from moss. The isolated enzymes strongly recognize and cleave a variety of auxin conjugates, including those of indole butyric and indole propionic acids. These P. patens hydrolases not only appear to be “cryptic”, but they are likely to have derived from soil bacteria through Horizontal Gene Transfer. Additionally, support is presented that the plant-type M20D peptidase family may have been universally lost from mosses after divergence from the common ancestor with liverwort.

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