Expression, purification and enzymatic characterization of Brugia malayi dihydrofolate reductase

Keyphrases

• Methylenetetrahydrofolate Reductase (MTHFR) 100%
• Brugia Malayi 100%
• Expression Characterization 100%
• Enzymatic Characterization 100%
• Purification Characterization 100%
• Catalytic Activity 33%
• Escherichia Coli 33%
• Lymphatic Filariasis 33%
• Kcat 33%
• Putative Drug Targets 33%
• Nicotinamide Adenine Dinucleotide Phosphate (NADPH) 33%
• Affinity Chromatography 33%
• IC50 Value 33%
• Trimethoprim 33%
• Pyrimethamine 33%
• Antifolate 33%
• Dihydrofolate 33%
• Methotrexate 33%
• Steady-state Kinetics 33%
• 2,4-Diaminopyrimidine 33%
• Kinetic Inhibition 33%
• DHFR Inhibitors 33%
• Kinetic Characterization 33%
• Equilibrium Dissociation Constant 33%
• Michaelis Constant 33%
• Inhibition Studies 33%
• His6 33%
• Cycloguanil 33%
• 2,4-diaminoquinazoline 33%
• Characterization Studies 33%
• Neglected Parasitic Diseases 33%
• Causative Agents 33%
• Recombinant Expression 33%

Biochemistry, Genetics and Molecular Biology

• Dihydrofolate Reductase 100%
• Brugia malayi 100%
• Copurification 100%
• Enzyme 33%
• Dissociation Constant 33%
• Steady State 33%
• Escherichia coli 33%
• Turnover Number 33%
• IC50 33%
• Antifolate 33%
• Michaelis Constant 33%
• Enzymatic Activity 33%
• Affinity Chromatography 33%
• Trimethoprim 33%
• Methotrexate 33%
• Pyrimethamine 33%

Pharmacology, Toxicology and Pharmaceutical Science

• Dihydrofolate Reductase 100%
• Brugia malayi 100%
• Lymphatic Filariasis 33%
• Escherichia coli 33%
• Parasitosis 33%
• Elimination 33%
• IC50 33%
• Dihydrofolic Acid 33%
• Folic Acid Antagonist 33%
• Pyrimethamine 33%
• Trimethoprim 33%
• Methotrexate 33%
• Cycloguanil 33%
• Reduced Nicotinamide Adenine Dinucleotide Phosphate 33%