Extracellular acidosis increases neuronal cell calcium by activating acid-sensing ion channel 1a

Olena Yermolaieva, A. Soren Leonard, Mikael K. Schnizler, Francois M. Abboud, Michael J. Welsh

Research output: Contribution to journalArticlepeer-review

345 Scopus citations


Acid-sensing ion channel (ASIC) 1a subunit is expressed in synapses of central neurons where it contributes to synaptic plasticity. However, whether these channels can conduct Ca2+ and thereby raise the cytosolic Ca2+ concentration, [Ca2+]c, and possibly alter neuronal physiology has been uncertain. We found that extracellular acidosis opened ASIC1a channels, which provided a pathway for Ca2+ entry and elevated [Ca2+]c in wild-type, but not ASIC1 -/-, hippocampal neurons. Acid application also raised [Ca 2+]c and evoked Ca2+ currents in heterologous cells expressing ASIC1a. Although ASIC2a is also expressed in central neurons, neither ASIC2a homomultimeric channels nor ASIC1a/2a heteromultimers showed H+-activated [Ca2+]c elevation or Ca 2+ currents. Because extracellular acidosis accompanying cerebral ischemia contributes to neuronal injury, we tested the effect of acidosis on cell death measured as lactate dehydrogenase release. Eliminating ASIC1a from neurons or treating ASIC1a-expressing cells with the ASIC blocker amiloride attenuated acidosis-induced cell injury. These results indicate that ASIC1a provides a non-voltage-gated pathway for Ca2+ to enter neurons. Thus, it may provide a target for modulation of [Ca2+]c.

Original languageEnglish
Pages (from-to)6752-6757
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Issue number17
StatePublished - 27 Apr 2004


Dive into the research topics of 'Extracellular acidosis increases neuronal cell calcium by activating acid-sensing ion channel 1a'. Together they form a unique fingerprint.

Cite this