Extrusion of calcium from a single isolated neuron of the snail Helix pomatia

A. V. Tepikin; P. G. Kostyuk; V. A. Snitsarev; P. V. Belan

doi:10.1007/BF01993961

Extrusion of calcium from a single isolated neuron of the snail Helix pomatia

A. V. Tepikin, P. G. Kostyuk, V. A. Snitsarev, P. V. Belan

Biology

Research output: Contribution to journal › Article › peer-review

18 Scopus citations

Abstract

Simultaneous optical measurements of extra- and intracellular Ca²⁺ concentrations were carried out on isolated snail neurons injected iontophoretically with Ca²⁺. The fluorescent indicator Fura-2 was used to measure intracellular concentration of free Ca, and the absorbant indicator Antipyrylazo III to measure changes in extracellular calcium concentration in the microchamber containing the cell. The velocity of Ca²⁺ extrusion from a single cell has been shown to be in accordance with the level of free Ca in the neuronal cytoplasm. After an increase in intracellular free Ca by iontophoretic injection from a microeletrode to 0.2-0.5 μm, the velocity of Ca²⁺ extrusion from the neuron was approximately 0.3-4.6 μm/sec per cell volume. During caffeine-induced calcium-dependent calcium release of Ca²⁺ from intracellular stores a stimulation of calcium extrusion took place, reaching the velocity of 5.0 μm/sec per cell volume.

Original language	English
Pages (from-to)	43-47
Number of pages	5
Journal	The Journal of Membrane Biology
Volume	123
Issue number	1
DOIs	https://doi.org/10.1007/BF01993961
State	Published - Jul 1991

Keywords

Antipyrylazo III absorbance
Fura-2 fluorescence
Helix pomatia neurons
calcium extrusion

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title = "Extrusion of calcium from a single isolated neuron of the snail Helix pomatia",

abstract = "Simultaneous optical measurements of extra- and intracellular Ca2+ concentrations were carried out on isolated snail neurons injected iontophoretically with Ca2+. The fluorescent indicator Fura-2 was used to measure intracellular concentration of free Ca, and the absorbant indicator Antipyrylazo III to measure changes in extracellular calcium concentration in the microchamber containing the cell. The velocity of Ca2+ extrusion from a single cell has been shown to be in accordance with the level of free Ca in the neuronal cytoplasm. After an increase in intracellular free Ca by iontophoretic injection from a microeletrode to 0.2-0.5 μm, the velocity of Ca2+ extrusion from the neuron was approximately 0.3-4.6 μm/sec per cell volume. During caffeine-induced calcium-dependent calcium release of Ca2+ from intracellular stores a stimulation of calcium extrusion took place, reaching the velocity of 5.0 μm/sec per cell volume.",

keywords = "Antipyrylazo III absorbance, Fura-2 fluorescence, Helix pomatia neurons, calcium extrusion",

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AU - Tepikin, A. V.

AU - Kostyuk, P. G.

AU - Snitsarev, V. A.

AU - Belan, P. V.

PY - 1991/7

Y1 - 1991/7

N2 - Simultaneous optical measurements of extra- and intracellular Ca2+ concentrations were carried out on isolated snail neurons injected iontophoretically with Ca2+. The fluorescent indicator Fura-2 was used to measure intracellular concentration of free Ca, and the absorbant indicator Antipyrylazo III to measure changes in extracellular calcium concentration in the microchamber containing the cell. The velocity of Ca2+ extrusion from a single cell has been shown to be in accordance with the level of free Ca in the neuronal cytoplasm. After an increase in intracellular free Ca by iontophoretic injection from a microeletrode to 0.2-0.5 μm, the velocity of Ca2+ extrusion from the neuron was approximately 0.3-4.6 μm/sec per cell volume. During caffeine-induced calcium-dependent calcium release of Ca2+ from intracellular stores a stimulation of calcium extrusion took place, reaching the velocity of 5.0 μm/sec per cell volume.

AB - Simultaneous optical measurements of extra- and intracellular Ca2+ concentrations were carried out on isolated snail neurons injected iontophoretically with Ca2+. The fluorescent indicator Fura-2 was used to measure intracellular concentration of free Ca, and the absorbant indicator Antipyrylazo III to measure changes in extracellular calcium concentration in the microchamber containing the cell. The velocity of Ca2+ extrusion from a single cell has been shown to be in accordance with the level of free Ca in the neuronal cytoplasm. After an increase in intracellular free Ca by iontophoretic injection from a microeletrode to 0.2-0.5 μm, the velocity of Ca2+ extrusion from the neuron was approximately 0.3-4.6 μm/sec per cell volume. During caffeine-induced calcium-dependent calcium release of Ca2+ from intracellular stores a stimulation of calcium extrusion took place, reaching the velocity of 5.0 μm/sec per cell volume.

KW - Antipyrylazo III absorbance

KW - Fura-2 fluorescence

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Extrusion of calcium from a single isolated neuron of the snail Helix pomatia

Abstract

Keywords

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