Folding dynamics of single GCN-4 peptides by fluorescence resonant energy transfer confocal microscopy

Yiwei Jia, David Talaga, Wai Leung Lau, Helen S.M. Lu, William F. DeGrado, Robin M. Hochstrasser

Research output: Contribution to journalArticle

147 Citations (Scopus)

Abstract

We have prepared a bichromophoric crosslinked variant of GCN4-P1 for single-molecule fluorescence energy transfer experiments (GCN4-Pf). The folding and unfolding fluctuations of single GCN4-Pf molecules are measured in a two-channel confocal microscope with which donor and acceptor fluorescence trajectories are measured simultaneously. The energy transfer efficiency is thereby determined and its probability distributions as a function of added denaturant [urea] are calculated. The distributions indicate that single molecule GCN4-Pf is in dynamic folding equilibrium with the position of the equilibrium being altered by the concentration of urea.

Original languageEnglish
Pages (from-to)69-83
Number of pages15
JournalChemical Physics
Volume247
Issue number1
DOIs
StatePublished - 1 Aug 1999

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Confocal microscopy
Energy transfer
folding
peptides
Fluorescence
energy transfer
microscopy
ureas
fluorescence
Peptides
Molecules
Urea
molecules
Probability distributions
Microscopes
microscopes
Trajectories
trajectories
Experiments

Cite this

Jia, Yiwei ; Talaga, David ; Lau, Wai Leung ; Lu, Helen S.M. ; DeGrado, William F. ; Hochstrasser, Robin M. / Folding dynamics of single GCN-4 peptides by fluorescence resonant energy transfer confocal microscopy. In: Chemical Physics. 1999 ; Vol. 247, No. 1. pp. 69-83.
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Folding dynamics of single GCN-4 peptides by fluorescence resonant energy transfer confocal microscopy. / Jia, Yiwei; Talaga, David; Lau, Wai Leung; Lu, Helen S.M.; DeGrado, William F.; Hochstrasser, Robin M.

In: Chemical Physics, Vol. 247, No. 1, 01.08.1999, p. 69-83.

Research output: Contribution to journalArticle

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