Genetic characterization of Anaplasma phagocytophilum strains from goats (Capra aegagrus hircus) and water buffalo (Bubalus bubalis) by 16S rRNA gene, ankA gene and multilocus sequence typing

Denis B. Langenwalder, Sabine Schmidt, Urs Gilli, Nikola Pantchev, Martin Ganter, Cornelia Silaghi, Matthew Aardema, Friederike D. von Loewenich

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Anaplasma phagocytophilum is a Gram-negative obligate intracellular bacterium that replicates in neutrophil granulocytes. It is transmitted by ticks and causes tick-borne fever in domestic ruminants such as sheep, cattle and goats. However, in contrast to sheep and cattle little is known about the clinical course of infection in goats. We report here on three cases of symptomatic infection with A. phagocytophilum in two goats (Capra aegagrus hircus) and one water buffalo (Bubalus bubalis). The animals showed symptoms and laboratory findings similar to sheep and cattle. To our knowledge, this is the first report on the symptomatic infection of water buffalos with A. phagocytophilum. The infecting strains were genetically characterized by 16S rRNA gene, ankA gene and multilocus sequence typing (MLST). Four other strains from asymptomatically infected goats were also included. The ankA sequences from five goats were part of the formerly described ankA gene clusters I and IV that are known to contain A. phagocytophilum strains from sheep and cattle. However, the sequences from one goat and from the water buffalo belonged to ankA gene cluster II that was formerly described to be restricted to roe deer. A similar observation was made for MLST as three goats clustered with sequences from sheep and cattle, whereas three other goats and the water buffalo were found to be part of the roe deer cluster. However, since most of the strains from sheep and cattle were distinct from the roe deer strains, roe deer might not represent major reservoir hosts for tick-borne fever in domestic ruminants. When differing parts of the 16S rRNA gene were used for typing the results were conflicting. This shows that the use of a standardized typing method such as MLST is highly desirable to generate easily comparable results.

Original languageEnglish
Article number101267
JournalTicks and Tick-borne Diseases
Volume10
Issue number6
DOIs
StatePublished - 1 Oct 2019

Fingerprint

Anaplasma phagocytophilum
Multilocus Sequence Typing
Buffaloes
rRNA Genes
Goats
buffaloes
goats
ribosomal RNA
Capreolus capreolus
Sheep
Deer
sheep
Genes
cattle
genes
Ticks
ticks
multigene family
Ruminants
Multigene Family

Keywords

  • 16S rRNA
  • Anaplasma phagocytophilum
  • Goat
  • MLST
  • Water buffalo
  • ankA

Cite this

Langenwalder, Denis B. ; Schmidt, Sabine ; Gilli, Urs ; Pantchev, Nikola ; Ganter, Martin ; Silaghi, Cornelia ; Aardema, Matthew ; von Loewenich, Friederike D. / Genetic characterization of Anaplasma phagocytophilum strains from goats (Capra aegagrus hircus) and water buffalo (Bubalus bubalis) by 16S rRNA gene, ankA gene and multilocus sequence typing. In: Ticks and Tick-borne Diseases. 2019 ; Vol. 10, No. 6.
@article{418322fdf13a45f49a61e9a74623d6c4,
title = "Genetic characterization of Anaplasma phagocytophilum strains from goats (Capra aegagrus hircus) and water buffalo (Bubalus bubalis) by 16S rRNA gene, ankA gene and multilocus sequence typing",
abstract = "Anaplasma phagocytophilum is a Gram-negative obligate intracellular bacterium that replicates in neutrophil granulocytes. It is transmitted by ticks and causes tick-borne fever in domestic ruminants such as sheep, cattle and goats. However, in contrast to sheep and cattle little is known about the clinical course of infection in goats. We report here on three cases of symptomatic infection with A. phagocytophilum in two goats (Capra aegagrus hircus) and one water buffalo (Bubalus bubalis). The animals showed symptoms and laboratory findings similar to sheep and cattle. To our knowledge, this is the first report on the symptomatic infection of water buffalos with A. phagocytophilum. The infecting strains were genetically characterized by 16S rRNA gene, ankA gene and multilocus sequence typing (MLST). Four other strains from asymptomatically infected goats were also included. The ankA sequences from five goats were part of the formerly described ankA gene clusters I and IV that are known to contain A. phagocytophilum strains from sheep and cattle. However, the sequences from one goat and from the water buffalo belonged to ankA gene cluster II that was formerly described to be restricted to roe deer. A similar observation was made for MLST as three goats clustered with sequences from sheep and cattle, whereas three other goats and the water buffalo were found to be part of the roe deer cluster. However, since most of the strains from sheep and cattle were distinct from the roe deer strains, roe deer might not represent major reservoir hosts for tick-borne fever in domestic ruminants. When differing parts of the 16S rRNA gene were used for typing the results were conflicting. This shows that the use of a standardized typing method such as MLST is highly desirable to generate easily comparable results.",
keywords = "16S rRNA, Anaplasma phagocytophilum, Goat, MLST, Water buffalo, ankA",
author = "Langenwalder, {Denis B.} and Sabine Schmidt and Urs Gilli and Nikola Pantchev and Martin Ganter and Cornelia Silaghi and Matthew Aardema and {von Loewenich}, {Friederike D.}",
year = "2019",
month = "10",
day = "1",
doi = "10.1016/j.ttbdis.2019.101267",
language = "English",
volume = "10",
journal = "Ticks and Tick-borne Diseases",
issn = "1877-959X",
number = "6",

}

Genetic characterization of Anaplasma phagocytophilum strains from goats (Capra aegagrus hircus) and water buffalo (Bubalus bubalis) by 16S rRNA gene, ankA gene and multilocus sequence typing. / Langenwalder, Denis B.; Schmidt, Sabine; Gilli, Urs; Pantchev, Nikola; Ganter, Martin; Silaghi, Cornelia; Aardema, Matthew; von Loewenich, Friederike D.

In: Ticks and Tick-borne Diseases, Vol. 10, No. 6, 101267, 01.10.2019.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Genetic characterization of Anaplasma phagocytophilum strains from goats (Capra aegagrus hircus) and water buffalo (Bubalus bubalis) by 16S rRNA gene, ankA gene and multilocus sequence typing

AU - Langenwalder, Denis B.

AU - Schmidt, Sabine

AU - Gilli, Urs

AU - Pantchev, Nikola

AU - Ganter, Martin

AU - Silaghi, Cornelia

AU - Aardema, Matthew

AU - von Loewenich, Friederike D.

PY - 2019/10/1

Y1 - 2019/10/1

N2 - Anaplasma phagocytophilum is a Gram-negative obligate intracellular bacterium that replicates in neutrophil granulocytes. It is transmitted by ticks and causes tick-borne fever in domestic ruminants such as sheep, cattle and goats. However, in contrast to sheep and cattle little is known about the clinical course of infection in goats. We report here on three cases of symptomatic infection with A. phagocytophilum in two goats (Capra aegagrus hircus) and one water buffalo (Bubalus bubalis). The animals showed symptoms and laboratory findings similar to sheep and cattle. To our knowledge, this is the first report on the symptomatic infection of water buffalos with A. phagocytophilum. The infecting strains were genetically characterized by 16S rRNA gene, ankA gene and multilocus sequence typing (MLST). Four other strains from asymptomatically infected goats were also included. The ankA sequences from five goats were part of the formerly described ankA gene clusters I and IV that are known to contain A. phagocytophilum strains from sheep and cattle. However, the sequences from one goat and from the water buffalo belonged to ankA gene cluster II that was formerly described to be restricted to roe deer. A similar observation was made for MLST as three goats clustered with sequences from sheep and cattle, whereas three other goats and the water buffalo were found to be part of the roe deer cluster. However, since most of the strains from sheep and cattle were distinct from the roe deer strains, roe deer might not represent major reservoir hosts for tick-borne fever in domestic ruminants. When differing parts of the 16S rRNA gene were used for typing the results were conflicting. This shows that the use of a standardized typing method such as MLST is highly desirable to generate easily comparable results.

AB - Anaplasma phagocytophilum is a Gram-negative obligate intracellular bacterium that replicates in neutrophil granulocytes. It is transmitted by ticks and causes tick-borne fever in domestic ruminants such as sheep, cattle and goats. However, in contrast to sheep and cattle little is known about the clinical course of infection in goats. We report here on three cases of symptomatic infection with A. phagocytophilum in two goats (Capra aegagrus hircus) and one water buffalo (Bubalus bubalis). The animals showed symptoms and laboratory findings similar to sheep and cattle. To our knowledge, this is the first report on the symptomatic infection of water buffalos with A. phagocytophilum. The infecting strains were genetically characterized by 16S rRNA gene, ankA gene and multilocus sequence typing (MLST). Four other strains from asymptomatically infected goats were also included. The ankA sequences from five goats were part of the formerly described ankA gene clusters I and IV that are known to contain A. phagocytophilum strains from sheep and cattle. However, the sequences from one goat and from the water buffalo belonged to ankA gene cluster II that was formerly described to be restricted to roe deer. A similar observation was made for MLST as three goats clustered with sequences from sheep and cattle, whereas three other goats and the water buffalo were found to be part of the roe deer cluster. However, since most of the strains from sheep and cattle were distinct from the roe deer strains, roe deer might not represent major reservoir hosts for tick-borne fever in domestic ruminants. When differing parts of the 16S rRNA gene were used for typing the results were conflicting. This shows that the use of a standardized typing method such as MLST is highly desirable to generate easily comparable results.

KW - 16S rRNA

KW - Anaplasma phagocytophilum

KW - Goat

KW - MLST

KW - Water buffalo

KW - ankA

UR - http://www.scopus.com/inward/record.url?scp=85070889470&partnerID=8YFLogxK

U2 - 10.1016/j.ttbdis.2019.101267

DO - 10.1016/j.ttbdis.2019.101267

M3 - Article

VL - 10

JO - Ticks and Tick-borne Diseases

JF - Ticks and Tick-borne Diseases

SN - 1877-959X

IS - 6

M1 - 101267

ER -