Identification and separation of five cephalosporins by micellar electrokinetic capillary chromatography

Carl J. Sciacchitano, Barry Mopper, John Specchio

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

The cephalosporins are a group of structurally related, broad spectrum β-lactam antibiotics isolated from the mold Cephalosporium. Methods of analysis of cephalosporin antibiotics include microbiological, titrimetric and chromatographic assays. Chromatographic techniques, including high-performance liquid chromatography, have been extensively utilized for specific and sensitive assays of β-lactam antibiotics in a variety of matrices, i.e. clinical and pharmaceutical. Several of the drawbacks of HPLC in the analysis of cephalosporins in food and biological samples include matrix interferences and low resolution due to column adsorption. Recently, the applicability of capillary electrophoresis to the resolution of β-lactam antibiotics has been demonstrated in the literature. In this paper we employed sodium borate and an anionic surfactant, sodium dodecyl sulfate (SDS), in a separation technique called micellar electrokinetic capillary chromatography with UV detection, to resolve a mixture of five cephalosporins -cefuroxime, cephalexin, cephapirin, cefamandole nafate and cephalothin. The presence of SDS in the running buffer above the critical micelle concentration, creates a pseudostationary phase enabling high-efficiency chromatographic separations. The effect of the ion-pairing reagent, pentanesulfonic acid sodium salt, on the resolution of the cephalosporin mixture in conjunction with SDS was also examined.

Original languageEnglish
Pages (from-to)395-399
Number of pages5
JournalJournal of Chromatography B: Biomedical Sciences and Applications
Volume657
Issue number2
DOIs
StatePublished - 15 Jul 1994

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Cephalosporins
Chromatography
Lactams
Sodium Dodecyl Sulfate
Anti-Bacterial Agents
Assays
Cephapirin
Cephalexin
Cephalothin
Cefuroxime
Capillary electrophoresis
Anionic surfactants
Critical micelle concentration
High performance liquid chromatography
Buffers
Salts
Sodium
Ions
Adsorption
Acids

Cite this

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abstract = "The cephalosporins are a group of structurally related, broad spectrum β-lactam antibiotics isolated from the mold Cephalosporium. Methods of analysis of cephalosporin antibiotics include microbiological, titrimetric and chromatographic assays. Chromatographic techniques, including high-performance liquid chromatography, have been extensively utilized for specific and sensitive assays of β-lactam antibiotics in a variety of matrices, i.e. clinical and pharmaceutical. Several of the drawbacks of HPLC in the analysis of cephalosporins in food and biological samples include matrix interferences and low resolution due to column adsorption. Recently, the applicability of capillary electrophoresis to the resolution of β-lactam antibiotics has been demonstrated in the literature. In this paper we employed sodium borate and an anionic surfactant, sodium dodecyl sulfate (SDS), in a separation technique called micellar electrokinetic capillary chromatography with UV detection, to resolve a mixture of five cephalosporins -cefuroxime, cephalexin, cephapirin, cefamandole nafate and cephalothin. The presence of SDS in the running buffer above the critical micelle concentration, creates a pseudostationary phase enabling high-efficiency chromatographic separations. The effect of the ion-pairing reagent, pentanesulfonic acid sodium salt, on the resolution of the cephalosporin mixture in conjunction with SDS was also examined.",
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Identification and separation of five cephalosporins by micellar electrokinetic capillary chromatography. / Sciacchitano, Carl J.; Mopper, Barry; Specchio, John.

In: Journal of Chromatography B: Biomedical Sciences and Applications, Vol. 657, No. 2, 15.07.1994, p. 395-399.

Research output: Contribution to journalArticle

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