Identification of a Drosophila 60S ribosomal protein

John Gaynor, Martin G. Fox

Research output: Contribution to journalArticleResearchpeer-review

Abstract

We have previously reported the cloning and sequencing of a cDNA from Drosophila (DL43; GenBank Accession U40226) which predicts a small protein homologous to a ribosomal protein in yeast and rat. In this study we show that a 330 nucleotide transcript encoding the putative ribosomal protein is expressed in Drosophila Kc cells under normal growth conditions (25°C). This RNA is associated with the translational machinery and is present on polysomes. The DL43 cDNA was transcribed using T7 RNA polymerase and the resulting transcripts translated in a wheat germ extract. SDS/PAGE analysis of the in vitro transcribed and translated DL43 cDNA yields a small protein of approximately 9 kDa. Using two-dimensional gel-electrophoresis we have established that the radiolabeled DL43 protein comigrates with a small basic protein in the 60S ribosomal subunit. The electrophoretic mobilities of the in vitro and in vivo synthesized DL43 proteins are indistinguishable providing support for the identity of this protein.

Original languageEnglish
Pages (from-to)223-228
Number of pages6
JournalInsect Biochemistry and Molecular Biology
Volume27
Issue number3
DOIs
StatePublished - 1 Jan 1997

Fingerprint

Ribosomal Proteins
ribosomal proteins
Drosophila
Proteins
proteins
Complementary DNA
Eukaryotic Large Ribosome Subunits
polyribosomes
wheat germ
two-dimensional gel electrophoresis
DNA-directed RNA polymerase
Electrophoretic mobility
Polyribosomes
Cloning
Nucleic Acid Databases
Electrophoresis, Gel, Two-Dimensional
polyacrylamide gel electrophoresis
molecular cloning
Electrophoresis
Yeast

Keywords

  • 60S ribosomal subunit
  • Drosophila
  • Ribosomal protein

Cite this

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Identification of a Drosophila 60S ribosomal protein. / Gaynor, John; Fox, Martin G.

In: Insect Biochemistry and Molecular Biology, Vol. 27, No. 3, 01.01.1997, p. 223-228.

Research output: Contribution to journalArticleResearchpeer-review

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