Identification of histidine 105 in the β1 subunit of soluble guanylate cyclase as the heme proximal ligand

Yunde Zhao, Johannes Schelvis, Gerald T. Babcock, Michael A. Marletta

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Abstract

Soluble guanylate cyclase isolated from bovine and rat lung is a heterodimeric hemoprotein composed of α1 and β1 subunits. The heme binding region has been localized to residues 1-385 of the β1 subunit [β1(1-385)], while the catalytic site(s) have been localized to the C-terminal region of sGC. There are four conserved histidine residues in the heme binding region of sGC. H220 and H346 are conserved among all known sGC subunits (α and β), while H105 and H134 are conserved only in the β subunits (β1 and β2). Site-directed mutagenesis was used to individually change each of the conserved histidines in sGC β1(1-385) to alanine or glycine, and the resulting mutants were expressed in E. coli. All of the mutants except for H105A and H105G had heme bound as isolated. Imidazole (Im) was able to rescue heme binding to H105G when added to the growth medium and purification buffers. The heme in H105G isolated in the presence of imidazole [H105G(Im)] was ferric and a mixture of 5-coordinate, high-spin and 6-coordinate, low- spin complexes. After reduction, the ferrous heme in H105G(Im) was 5- coordinate, high-spin as indicated by resonance Raman spectroscopy. When imidazole in H105G(Im) was exchanged with N-methylimidazole (MeIm), the Fe- N(Im/MeIm) stretching frequency was shifted from 221 to 212 cm-1. A shift of this magnitude is expected when the ligand is directly coordinated to the heme iron. All of the data are consistent with the conclusion that H105 in the β1 subunit is the heme proximal ligand.

Original languageEnglish
Pages (from-to)4502-4509
Number of pages8
JournalBiochemistry
Volume37
Issue number13
DOIs
StatePublished - 31 Mar 1998

Fingerprint

Guanylate Cyclase
Heme
Histidine
Ligands
Mutagenesis
Soluble Guanylyl Cyclase
Raman Spectrum Analysis
Site-Directed Mutagenesis
imidazole
Alanine
Glycine
Escherichia coli
Stretching
Purification
Raman spectroscopy
Rats
Catalytic Domain
Buffers
Iron
Lung

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Zhao, Yunde ; Schelvis, Johannes ; Babcock, Gerald T. ; Marletta, Michael A. / Identification of histidine 105 in the β1 subunit of soluble guanylate cyclase as the heme proximal ligand. In: Biochemistry. 1998 ; Vol. 37, No. 13. pp. 4502-4509.
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abstract = "Soluble guanylate cyclase isolated from bovine and rat lung is a heterodimeric hemoprotein composed of α1 and β1 subunits. The heme binding region has been localized to residues 1-385 of the β1 subunit [β1(1-385)], while the catalytic site(s) have been localized to the C-terminal region of sGC. There are four conserved histidine residues in the heme binding region of sGC. H220 and H346 are conserved among all known sGC subunits (α and β), while H105 and H134 are conserved only in the β subunits (β1 and β2). Site-directed mutagenesis was used to individually change each of the conserved histidines in sGC β1(1-385) to alanine or glycine, and the resulting mutants were expressed in E. coli. All of the mutants except for H105A and H105G had heme bound as isolated. Imidazole (Im) was able to rescue heme binding to H105G when added to the growth medium and purification buffers. The heme in H105G isolated in the presence of imidazole [H105G(Im)] was ferric and a mixture of 5-coordinate, high-spin and 6-coordinate, low- spin complexes. After reduction, the ferrous heme in H105G(Im) was 5- coordinate, high-spin as indicated by resonance Raman spectroscopy. When imidazole in H105G(Im) was exchanged with N-methylimidazole (MeIm), the Fe- N(Im/MeIm) stretching frequency was shifted from 221 to 212 cm-1. A shift of this magnitude is expected when the ligand is directly coordinated to the heme iron. All of the data are consistent with the conclusion that H105 in the β1 subunit is the heme proximal ligand.",
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Identification of histidine 105 in the β1 subunit of soluble guanylate cyclase as the heme proximal ligand. / Zhao, Yunde; Schelvis, Johannes; Babcock, Gerald T.; Marletta, Michael A.

In: Biochemistry, Vol. 37, No. 13, 31.03.1998, p. 4502-4509.

Research output: Contribution to journalArticle

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T1 - Identification of histidine 105 in the β1 subunit of soluble guanylate cyclase as the heme proximal ligand

AU - Zhao, Yunde

AU - Schelvis, Johannes

AU - Babcock, Gerald T.

AU - Marletta, Michael A.

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AB - Soluble guanylate cyclase isolated from bovine and rat lung is a heterodimeric hemoprotein composed of α1 and β1 subunits. The heme binding region has been localized to residues 1-385 of the β1 subunit [β1(1-385)], while the catalytic site(s) have been localized to the C-terminal region of sGC. There are four conserved histidine residues in the heme binding region of sGC. H220 and H346 are conserved among all known sGC subunits (α and β), while H105 and H134 are conserved only in the β subunits (β1 and β2). Site-directed mutagenesis was used to individually change each of the conserved histidines in sGC β1(1-385) to alanine or glycine, and the resulting mutants were expressed in E. coli. All of the mutants except for H105A and H105G had heme bound as isolated. Imidazole (Im) was able to rescue heme binding to H105G when added to the growth medium and purification buffers. The heme in H105G isolated in the presence of imidazole [H105G(Im)] was ferric and a mixture of 5-coordinate, high-spin and 6-coordinate, low- spin complexes. After reduction, the ferrous heme in H105G(Im) was 5- coordinate, high-spin as indicated by resonance Raman spectroscopy. When imidazole in H105G(Im) was exchanged with N-methylimidazole (MeIm), the Fe- N(Im/MeIm) stretching frequency was shifted from 221 to 212 cm-1. A shift of this magnitude is expected when the ligand is directly coordinated to the heme iron. All of the data are consistent with the conclusion that H105 in the β1 subunit is the heme proximal ligand.

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