Imaging synaptic activity in intact brain and slices with FM1-43 in C. elegans, lamprey, and rat

Alan R. Kay; Aixa Alfonso; Simon Alford; Hollis T. Cline; Andrea M. Holgado; Bert Sakmann; Vladislav A. Snitsarev; Thomas P. Stricker; Michiko Takahashi; Ling Gang Wu

doi:10.1016/S0896-6273(00)81029-6

Imaging synaptic activity in intact brain and slices with FM1-43 in C. elegans, lamprey, and rat

Alan R. Kay, Aixa Alfonso, Simon Alford, Hollis T. Cline, Andrea M. Holgado, Bert Sakmann, Vladislav A. Snitsarev, Thomas P. Stricker, Michiko Takahashi, Ling Gang Wu

Biology

Research output: Contribution to journal › Article › peer-review

138 Scopus citations

Abstract

The fluorescent probe FM1-43 has been used extensively for imaging vesicle recycling; however, high nonspecific adsorption resulting in elevated background levels has precluded its use in certain tissues, notably brain slices. We have found that a sulfobutylated derivative of β-cyclodextrin (ADVASEP-7) has a higher affinity for FM1-43 than the plasma membrane. ADVASEP-7 was used as a carrier to remove FM1-43 nonspecifically bound to the outer leaflet of the plasma membrane or extracellular molecules, significantly reducing background staining. This has enabled us to visualize synaptic vesicle recycling in the nematode C. elegans, intact lamprey spinal cord, and rat brain slices.

Original language	English
Pages (from-to)	809-817
Number of pages	9
Journal	Neuron
Volume	24
Issue number	4
DOIs	https://doi.org/10.1016/S0896-6273(00)81029-6
State	Published - Dec 1999

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title = "Imaging synaptic activity in intact brain and slices with FM1-43 in C. elegans, lamprey, and rat",

abstract = "The fluorescent probe FM1-43 has been used extensively for imaging vesicle recycling; however, high nonspecific adsorption resulting in elevated background levels has precluded its use in certain tissues, notably brain slices. We have found that a sulfobutylated derivative of β-cyclodextrin (ADVASEP-7) has a higher affinity for FM1-43 than the plasma membrane. ADVASEP-7 was used as a carrier to remove FM1-43 nonspecifically bound to the outer leaflet of the plasma membrane or extracellular molecules, significantly reducing background staining. This has enabled us to visualize synaptic vesicle recycling in the nematode C. elegans, intact lamprey spinal cord, and rat brain slices.",

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AU - Kay, Alan R.

AU - Alfonso, Aixa

AU - Alford, Simon

AU - Cline, Hollis T.

AU - Holgado, Andrea M.

AU - Sakmann, Bert

AU - Snitsarev, Vladislav A.

AU - Stricker, Thomas P.

AU - Takahashi, Michiko

AU - Wu, Ling Gang

PY - 1999/12

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Imaging synaptic activity in intact brain and slices with FM1-43 in C. elegans, lamprey, and rat

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