Immediate and long-term impacts of UV-C irradiation on photosynthetic capacity, survival and microcystin-LR release risk of Microcystis aeruginosa

Huase Ou, Naiyun Gao, Yang Deng, Junlian Qiao, Hao Wang

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Abstract

In this study, the immediate and long-term impacts of shortwave ultraviolet (UV-C) irradiation on photosynthetic capacity, survival, and recovery of Microcystis aeruginosa were investigated. The risk of microcystin-LR (MC-LR) release during irradiation was also estimated. The cell density was determined by a flow cytometry, and typical chlorophyll fluorescence parameters, including the effective quantum yield, photosynthetic efficiency and maximal electron transport rate, were measured by a pulse amplitude modulated (PAM) fluorometer. Under various UV-C dosages (140-4200mJ cm -2 ), photosynthetic capacities were reduced, to different degrees, accompanied by slight cytoclasis and complete degradation of extracellular MC-LR immediately after irradiation. In a 6-d cultivation following UV-C irradiation, cell density and extracellular MC-LR in the samples treated by 140mJcm -2 UV-C irradiation increased from 4.0×10 6 cellsmL -1 and 8μgL -1 to 5.1×10 6 cellsmL -1 and 20μgL -1 , respectively. Significant M. aeruginosa cytoclasis (cell density from 4.0×10 6 to 1.0×10 6 cellsmL -1 ) and MC-LR release (2-25μgL -1 ) occurred when the UV-C dosage reached 350mJcm -2 . Cell cytoclasis and MC-LR release were enhanced in the cultivated samples under higher UV-C dosages. Results revealed that photosynthetic parameters were useful tools to predict the recovery profiles of M. aeruginosa cells, and the MC-LR release risk should be considered after UV-C inactivation.

Original languageEnglish
Pages (from-to)1241-1250
Number of pages10
JournalWater Research
Volume46
Issue number4
DOIs
StatePublished - 15 Mar 2012

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irradiation
Irradiation
Fluorometers
Recovery
Flow cytometry
flow cytometry
Quantum yield
Chlorophyll
chlorophyll
fluorescence
Fluorescence
Degradation
electron
degradation
dosage
parameter

Keywords

  • Cyanobacteria
  • Microcystin-LR
  • Microcystis aeruginosa
  • Photosynthesis
  • UV-C
  • Ultraviolet irradiation

Cite this

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title = "Immediate and long-term impacts of UV-C irradiation on photosynthetic capacity, survival and microcystin-LR release risk of Microcystis aeruginosa",
abstract = "In this study, the immediate and long-term impacts of shortwave ultraviolet (UV-C) irradiation on photosynthetic capacity, survival, and recovery of Microcystis aeruginosa were investigated. The risk of microcystin-LR (MC-LR) release during irradiation was also estimated. The cell density was determined by a flow cytometry, and typical chlorophyll fluorescence parameters, including the effective quantum yield, photosynthetic efficiency and maximal electron transport rate, were measured by a pulse amplitude modulated (PAM) fluorometer. Under various UV-C dosages (140-4200mJ cm -2 ), photosynthetic capacities were reduced, to different degrees, accompanied by slight cytoclasis and complete degradation of extracellular MC-LR immediately after irradiation. In a 6-d cultivation following UV-C irradiation, cell density and extracellular MC-LR in the samples treated by 140mJcm -2 UV-C irradiation increased from 4.0×10 6 cellsmL -1 and 8μgL -1 to 5.1×10 6 cellsmL -1 and 20μgL -1 , respectively. Significant M. aeruginosa cytoclasis (cell density from 4.0×10 6 to 1.0×10 6 cellsmL -1 ) and MC-LR release (2-25μgL -1 ) occurred when the UV-C dosage reached 350mJcm -2 . Cell cytoclasis and MC-LR release were enhanced in the cultivated samples under higher UV-C dosages. Results revealed that photosynthetic parameters were useful tools to predict the recovery profiles of M. aeruginosa cells, and the MC-LR release risk should be considered after UV-C inactivation.",
keywords = "Cyanobacteria, Microcystin-LR, Microcystis aeruginosa, Photosynthesis, UV-C, Ultraviolet irradiation",
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Immediate and long-term impacts of UV-C irradiation on photosynthetic capacity, survival and microcystin-LR release risk of Microcystis aeruginosa. / Ou, Huase; Gao, Naiyun; Deng, Yang; Qiao, Junlian; Wang, Hao.

In: Water Research, Vol. 46, No. 4, 15.03.2012, p. 1241-1250.

Research output: Contribution to journalArticleResearchpeer-review

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T1 - Immediate and long-term impacts of UV-C irradiation on photosynthetic capacity, survival and microcystin-LR release risk of Microcystis aeruginosa

AU - Ou, Huase

AU - Gao, Naiyun

AU - Deng, Yang

AU - Qiao, Junlian

AU - Wang, Hao

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N2 - In this study, the immediate and long-term impacts of shortwave ultraviolet (UV-C) irradiation on photosynthetic capacity, survival, and recovery of Microcystis aeruginosa were investigated. The risk of microcystin-LR (MC-LR) release during irradiation was also estimated. The cell density was determined by a flow cytometry, and typical chlorophyll fluorescence parameters, including the effective quantum yield, photosynthetic efficiency and maximal electron transport rate, were measured by a pulse amplitude modulated (PAM) fluorometer. Under various UV-C dosages (140-4200mJ cm -2 ), photosynthetic capacities were reduced, to different degrees, accompanied by slight cytoclasis and complete degradation of extracellular MC-LR immediately after irradiation. In a 6-d cultivation following UV-C irradiation, cell density and extracellular MC-LR in the samples treated by 140mJcm -2 UV-C irradiation increased from 4.0×10 6 cellsmL -1 and 8μgL -1 to 5.1×10 6 cellsmL -1 and 20μgL -1 , respectively. Significant M. aeruginosa cytoclasis (cell density from 4.0×10 6 to 1.0×10 6 cellsmL -1 ) and MC-LR release (2-25μgL -1 ) occurred when the UV-C dosage reached 350mJcm -2 . Cell cytoclasis and MC-LR release were enhanced in the cultivated samples under higher UV-C dosages. Results revealed that photosynthetic parameters were useful tools to predict the recovery profiles of M. aeruginosa cells, and the MC-LR release risk should be considered after UV-C inactivation.

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KW - Cyanobacteria

KW - Microcystin-LR

KW - Microcystis aeruginosa

KW - Photosynthesis

KW - UV-C

KW - Ultraviolet irradiation

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SP - 1241

EP - 1250

JO - Water Research

JF - Water Research

SN - 0043-1354

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