Inactivation and degradation of Microcystis aeruginosa by UV-C irradiation

Huase Ou, Naiyun Gao, Yang Deng, Hao Wang, Haixuan Zhang

Research output: Contribution to journalArticleResearchpeer-review

27 Citations (Scopus)

Abstract

In this study, the mechanisms and factors affecting the inactivation and degradation efficiency during UV-C irradiation of Microcystis aeruginosa, a harmful cyanobacteria strain, were investigated. Under different experimental conditions, the concentrations of three bioactivity materials, including protein, phycocyanin and chl-a, were measured, and fluorescence regional integration (FRI) was used to quantify the results of excitation emission matrix fluorescence spectroscopy. Furthermore, any alternation occurring in cell ultrastructure was determined using transmission electron microscopy. Results showed that UV-C could effectively damage the M. aeruginosa cells, most likely via a 3-step procedure, including impairment of photosynthesis system, decomposition of cytoplasmic inclusions, and cell cytoclasis. Comparison of FRI values and biochemical parameters in the presence of H 2 O 2 and HCO3- under the UV-C irradiation revealed the importance of photolysis and reactive oxygen species (ROS)-induced oxidation. UV-C/H 2 O 2 treatment was more efficient due to enhanced ROS generation, while adding HCO3- inhibited the ROS-induced oxidation, resulting in suppression on reaction. Humic acid and NO3-, two common water solutes, somewhat inhibited the inactivation and degradation processes, due to the ROS scavenging and " inner filter" effect.

Original languageEnglish
Pages (from-to)1192-1198
Number of pages7
JournalChemosphere
Volume85
Issue number7
DOIs
StatePublished - 1 Nov 2011

Fingerprint

Microcystis
Reactive Oxygen Species
irradiation
Irradiation
Degradation
degradation
Oxygen
fluorescence
Fluorescence
Phycocyanin
Humic Substances
oxidation
Oxidation
bioactivity
fluorescence spectroscopy
Photosynthesis
Photolysis
Fluorescence Spectrometry
Inclusion Bodies
Scavenging

Keywords

  • Cyanobacteria
  • Microcystis aeruginosa
  • Oxidation
  • Photolysis
  • UV-C

Cite this

Ou, Huase ; Gao, Naiyun ; Deng, Yang ; Wang, Hao ; Zhang, Haixuan. / Inactivation and degradation of Microcystis aeruginosa by UV-C irradiation. In: Chemosphere. 2011 ; Vol. 85, No. 7. pp. 1192-1198.
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Inactivation and degradation of Microcystis aeruginosa by UV-C irradiation. / Ou, Huase; Gao, Naiyun; Deng, Yang; Wang, Hao; Zhang, Haixuan.

In: Chemosphere, Vol. 85, No. 7, 01.11.2011, p. 1192-1198.

Research output: Contribution to journalArticleResearchpeer-review

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