Abstract
We use Shannon's definition of information to develop a theory to predict a photon-counting-based single-molecule experiment's ability to measure the desired property. We treat three phenomena that are commonly measured on single molecules: spectral fluctuations of a solvatochromic dye; assignment of the azimuthal dipole angle; determination of a distance by fluorescence resonant energy transfer using Förster's theory. We consider the effect of background and other "imperfections" on the measurement through the decrease in information.
| Original language | English |
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| Pages (from-to) | 9743-9757 |
| Number of pages | 15 |
| Journal | Journal of Physical Chemistry A |
| Volume | 110 |
| Issue number | 31 |
| DOIs | |
| State | Published - 10 Aug 2006 |