Inhibition of herpes simplex virus type 1 with the modified green tea polyphenol palmitoyl-epigallocatechin gallate

Aline De Oliveira, Sandra Adams, Lee Lee, Sean R. Murray, Stephen D. Hsu, Jeffrey R. Hammond, Douglas Dickinson, Ping Chen, Tin Chun Chu

Research output: Contribution to journalArticleResearchpeer-review

26 Citations (Scopus)

Abstract

Green tea polyphenol epigallocatechin gallate (EGCG) is a strong antioxidant that has previously been shown to reduce the number of plaques in HIV-infected cultured cells. Modified EGCG, palmitoyl-EGCG (p-EGCG), is of interest as a topical antiviral agent for herpes simplex virus (HSV-1) infections. This study evaluated the effect of p-EGCG on HSV-infected Vero cells. Results of cell viability and cell proliferation assays indicate that p-EGCG is not toxic to cultured Vero cells and show that modification of the green tea polyphenol epigallocatechin gallate (EGCG) with palmitate increases the effectiveness of EGCG as an antiviral agent. Furthermore, p-EGCG is a more potent inhibitor of herpes simplex virus 1 (HSV-1) than EGCG and can be topically applied to skin, one of the primary tissues infected by HSV. Viral binding assay, plaque forming assay, PCR, real-time PCR, and fluorescence microscopy were used to demonstrate that p-EGCG concentrations of 50 μM and higher block the production of infectious HSV-1 particles. p-EGCG was found to inhibit HSV-1 adsorption to Vero cells. Thus, p-EGCG may provide a novel treatment for HSV-1 infections.

Original languageEnglish
Pages (from-to)207-215
Number of pages9
JournalFood and Chemical Toxicology
Volume52
DOIs
StatePublished - 1 Feb 2013

Fingerprint

Human herpesvirus 1
epigallocatechin
Human Herpesvirus 1
Polyphenols
green tea
Tea
Viruses
polyphenols
Vero Cells
antiviral agents
Assays
Virus Diseases
Viral Plaque Assay
Antiviral Agents
Cultured Cells
assays
Cells
herpes simplex
Palmitates
Poisons

Keywords

  • DAPI
  • EGCG
  • GFP
  • HSV
  • HSV-1
  • HSV-2
  • P-EGCG
  • Palmitoyl-EGCG
  • Vero cells

Cite this

De Oliveira, Aline ; Adams, Sandra ; Lee, Lee ; Murray, Sean R. ; Hsu, Stephen D. ; Hammond, Jeffrey R. ; Dickinson, Douglas ; Chen, Ping ; Chu, Tin Chun. / Inhibition of herpes simplex virus type 1 with the modified green tea polyphenol palmitoyl-epigallocatechin gallate. In: Food and Chemical Toxicology. 2013 ; Vol. 52. pp. 207-215.
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Inhibition of herpes simplex virus type 1 with the modified green tea polyphenol palmitoyl-epigallocatechin gallate. / De Oliveira, Aline; Adams, Sandra; Lee, Lee; Murray, Sean R.; Hsu, Stephen D.; Hammond, Jeffrey R.; Dickinson, Douglas; Chen, Ping; Chu, Tin Chun.

In: Food and Chemical Toxicology, Vol. 52, 01.02.2013, p. 207-215.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Inhibition of herpes simplex virus type 1 with the modified green tea polyphenol palmitoyl-epigallocatechin gallate

AU - De Oliveira, Aline

AU - Adams, Sandra

AU - Lee, Lee

AU - Murray, Sean R.

AU - Hsu, Stephen D.

AU - Hammond, Jeffrey R.

AU - Dickinson, Douglas

AU - Chen, Ping

AU - Chu, Tin Chun

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AB - Green tea polyphenol epigallocatechin gallate (EGCG) is a strong antioxidant that has previously been shown to reduce the number of plaques in HIV-infected cultured cells. Modified EGCG, palmitoyl-EGCG (p-EGCG), is of interest as a topical antiviral agent for herpes simplex virus (HSV-1) infections. This study evaluated the effect of p-EGCG on HSV-infected Vero cells. Results of cell viability and cell proliferation assays indicate that p-EGCG is not toxic to cultured Vero cells and show that modification of the green tea polyphenol epigallocatechin gallate (EGCG) with palmitate increases the effectiveness of EGCG as an antiviral agent. Furthermore, p-EGCG is a more potent inhibitor of herpes simplex virus 1 (HSV-1) than EGCG and can be topically applied to skin, one of the primary tissues infected by HSV. Viral binding assay, plaque forming assay, PCR, real-time PCR, and fluorescence microscopy were used to demonstrate that p-EGCG concentrations of 50 μM and higher block the production of infectious HSV-1 particles. p-EGCG was found to inhibit HSV-1 adsorption to Vero cells. Thus, p-EGCG may provide a novel treatment for HSV-1 infections.

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