Abstract
Excessive concentrations of the eukaryotic initiation factor 2(eIF-2).stimulating protein, a factor that catalyzes the formation of binary (GTP.eIF-2) and ternary (GTP.eIF-2.initiator methionyl-tRNA) initiation complexes at physiological Mg2+ concentrations, can cause ternary complex dissociation when the Mg2+ concentration is raised from 0.5 to 5.0 mM. Stimulation of ternary complex formation and dissociation have similar (a) pH optima, (b) metal ion specificity, and (c) sensitivity to phosphorylation of the eIF-2 alpha subunit. The results suggest that ternary complex dissociation is an artifact with no physiological significance.
| Original language | English |
|---|---|
| Pages (from-to) | 4162-4165 |
| Number of pages | 4 |
| Journal | Journal of Biological Chemistry |
| Volume | 257 |
| Issue number | 8 |
| State | Published - 25 Apr 1982 |
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Initiation of protein synthesis in eukaryotes. Nature of ternary complex dissociation factor. / Siekierka, John; Datta, A.; Mauser, L.; Ochoa, S.
In: Journal of Biological Chemistry, Vol. 257, No. 8, 25.04.1982, p. 4162-4165.Research output: Contribution to journal › Article
TY - JOUR
T1 - Initiation of protein synthesis in eukaryotes. Nature of ternary complex dissociation factor.
AU - Siekierka, John
AU - Datta, A.
AU - Mauser, L.
AU - Ochoa, S.
PY - 1982/4/25
Y1 - 1982/4/25
N2 - Excessive concentrations of the eukaryotic initiation factor 2(eIF-2).stimulating protein, a factor that catalyzes the formation of binary (GTP.eIF-2) and ternary (GTP.eIF-2.initiator methionyl-tRNA) initiation complexes at physiological Mg2+ concentrations, can cause ternary complex dissociation when the Mg2+ concentration is raised from 0.5 to 5.0 mM. Stimulation of ternary complex formation and dissociation have similar (a) pH optima, (b) metal ion specificity, and (c) sensitivity to phosphorylation of the eIF-2 alpha subunit. The results suggest that ternary complex dissociation is an artifact with no physiological significance.
AB - Excessive concentrations of the eukaryotic initiation factor 2(eIF-2).stimulating protein, a factor that catalyzes the formation of binary (GTP.eIF-2) and ternary (GTP.eIF-2.initiator methionyl-tRNA) initiation complexes at physiological Mg2+ concentrations, can cause ternary complex dissociation when the Mg2+ concentration is raised from 0.5 to 5.0 mM. Stimulation of ternary complex formation and dissociation have similar (a) pH optima, (b) metal ion specificity, and (c) sensitivity to phosphorylation of the eIF-2 alpha subunit. The results suggest that ternary complex dissociation is an artifact with no physiological significance.
UR - http://www.scopus.com/inward/record.url?scp=0020490560&partnerID=8YFLogxK
M3 - Article
C2 - 6917851
AN - SCOPUS:0020490560
VL - 257
SP - 4162
EP - 4165
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
SN - 0021-9258
IS - 8
ER -