Initiation of protein synthesis in eukaryotes. Nature of ternary complex dissociation factor.

J. Siekierka; A. Datta; L. Mauser; S. Ochoa

Initiation of protein synthesis in eukaryotes. Nature of ternary complex dissociation factor.

J. Siekierka, A. Datta, L. Mauser, S. Ochoa

Chemistry and Biochemistry

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

Excessive concentrations of the eukaryotic initiation factor 2(eIF-2).stimulating protein, a factor that catalyzes the formation of binary (GTP.eIF-2) and ternary (GTP.eIF-2.initiator methionyl-tRNA) initiation complexes at physiological Mg2+ concentrations, can cause ternary complex dissociation when the Mg2+ concentration is raised from 0.5 to 5.0 mM. Stimulation of ternary complex formation and dissociation have similar (a) pH optima, (b) metal ion specificity, and (c) sensitivity to phosphorylation of the eIF-2 alpha subunit. The results suggest that ternary complex dissociation is an artifact with no physiological significance.

Original language	English
Pages (from-to)	4162-4165
Number of pages	4
Journal	Journal of Biological Chemistry
Volume	257
Issue number	8
State	Published - 25 Apr 1982

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title = "Initiation of protein synthesis in eukaryotes. Nature of ternary complex dissociation factor.",

abstract = "Excessive concentrations of the eukaryotic initiation factor 2(eIF-2).stimulating protein, a factor that catalyzes the formation of binary (GTP.eIF-2) and ternary (GTP.eIF-2.initiator methionyl-tRNA) initiation complexes at physiological Mg2+ concentrations, can cause ternary complex dissociation when the Mg2+ concentration is raised from 0.5 to 5.0 mM. Stimulation of ternary complex formation and dissociation have similar (a) pH optima, (b) metal ion specificity, and (c) sensitivity to phosphorylation of the eIF-2 alpha subunit. The results suggest that ternary complex dissociation is an artifact with no physiological significance.",

author = "J. Siekierka and A. Datta and L. Mauser and S. Ochoa",

year = "1982",

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language = "English",

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pages = "4162--4165",

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AU - Siekierka, J.

AU - Datta, A.

AU - Mauser, L.

AU - Ochoa, S.

PY - 1982/4/25

Y1 - 1982/4/25

N2 - Excessive concentrations of the eukaryotic initiation factor 2(eIF-2).stimulating protein, a factor that catalyzes the formation of binary (GTP.eIF-2) and ternary (GTP.eIF-2.initiator methionyl-tRNA) initiation complexes at physiological Mg2+ concentrations, can cause ternary complex dissociation when the Mg2+ concentration is raised from 0.5 to 5.0 mM. Stimulation of ternary complex formation and dissociation have similar (a) pH optima, (b) metal ion specificity, and (c) sensitivity to phosphorylation of the eIF-2 alpha subunit. The results suggest that ternary complex dissociation is an artifact with no physiological significance.

AB - Excessive concentrations of the eukaryotic initiation factor 2(eIF-2).stimulating protein, a factor that catalyzes the formation of binary (GTP.eIF-2) and ternary (GTP.eIF-2.initiator methionyl-tRNA) initiation complexes at physiological Mg2+ concentrations, can cause ternary complex dissociation when the Mg2+ concentration is raised from 0.5 to 5.0 mM. Stimulation of ternary complex formation and dissociation have similar (a) pH optima, (b) metal ion specificity, and (c) sensitivity to phosphorylation of the eIF-2 alpha subunit. The results suggest that ternary complex dissociation is an artifact with no physiological significance.

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