Isolation of a human cDNA encoding a 25 kDa FK-506 and rapamycin binding protein

Greg Wiederrecht, Mary M. Martin, Nolan H. Sigal, John J. Siekierka

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Recently, the nearly complete peptide sequence of a 25 kDa rapamycin and FK-506 binding protein that had been isolated from calf thymus, brain, and spleen was reported (1). Based upon the amino acid sequence of this bovine protein, bFKBP25, we have isolated from a JURKAT cDNA library the cDNA encoding the human homolog, hFKBP25. Translation of the open reading frame contained within this cDNA clone yields a sequence that, in its C-terminal half, is 41% identical to the major human FK-506 binding protein, hFKBP12, and 43% identical to hFKBP13. The N-terminal half of hFKBP25 is unrelated to any known protein.

Original languageEnglish
Pages (from-to)298-303
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume185
Issue number1
DOIs
StatePublished - 29 May 1992

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Tacrolimus Binding Proteins
Complementary DNA
Thymus
Sirolimus
Gene Library
Thymus Gland
Open Reading Frames
Amino Acid Sequence
Brain
Proteins
Spleen
Clone Cells
Amino Acids
Peptides

Cite this

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Isolation of a human cDNA encoding a 25 kDa FK-506 and rapamycin binding protein. / Wiederrecht, Greg; Martin, Mary M.; Sigal, Nolan H.; Siekierka, John J.

In: Biochemical and Biophysical Research Communications, Vol. 185, No. 1, 29.05.1992, p. 298-303.

Research output: Contribution to journalArticle

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