Isolation of an ILR1 auxin conjugate hydrolase homolog from Arabidopsis suecica

James Campanella, Vinela Bakllamaja, Tracie Restieri, Michael Vomacka, Jason Herron, Megan Patterson, Shahin Shahtaheri

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

We have isolated a homolog of the Arabidopsis thaliana IAA amidohydrolase ILR1 from the related species Arabidopsis suecica. Employing PCR primers targeted to the 5′ and 3′ untranslated regions of ILR1, A. suecica produced an amplified, genomic product (sILR1) of 2027 bp. The cloned sILR1 gene was sequenced, and the DNA homology between sILR1 and ILR1 found to be 98%. The sILR1 cDNA was isolated using RT-PCR, cloned and sequenced. The sILR1 cDNA was found to have 98% homology to its homolog ILR1 both at the DNA and amino acid level. The sILR1 mRNA transcript was found to be expressed in A. suecica tissues at ages 5, 10, and 15 days. Additionally, primers, designed to a ∼300 bp domain conserved in bacterial and plant amidohydrolases, were used to amplify a product from seven members of the Brassicaceae family (A. suecica, A. pumila, A. himalaica, A. griffithiana, A. wallichii, Draba nemoroza and Capsella bursa-pastoris), Lycopersicon esculentum, and two monocot species (Zea mays and Allium cepa) using PCR; this result suggests similar genes may be characterized in other species.

Original languageEnglish
Pages (from-to)175-181
Number of pages7
JournalPlant Growth Regulation
Volume39
Issue number2
DOIs
StatePublished - 1 Feb 2003

Fingerprint

amide hydrolases
Indoleacetic Acids
Hydrolases
hydrolases
Amidohydrolases
Arabidopsis
auxins
Draba
Capsella bursa-pastoris
Polymerase Chain Reaction
Capsella
5' untranslated regions
DNA
3' untranslated regions
Allium cepa
Complementary DNA
Liliopsida
Brassicaceae
Solanum lycopersicum var. lycopersicum
indole acetic acid

Keywords

  • Arabidopsis suecica
  • Arabidopsis thaliana
  • IAA amidohydrolase
  • IAA conjugate
  • Indole acetic acid
  • sILR1

Cite this

Campanella, J., Bakllamaja, V., Restieri, T., Vomacka, M., Herron, J., Patterson, M., & Shahtaheri, S. (2003). Isolation of an ILR1 auxin conjugate hydrolase homolog from Arabidopsis suecica. Plant Growth Regulation, 39(2), 175-181. https://doi.org/10.1023/A:1022528318188
Campanella, James ; Bakllamaja, Vinela ; Restieri, Tracie ; Vomacka, Michael ; Herron, Jason ; Patterson, Megan ; Shahtaheri, Shahin. / Isolation of an ILR1 auxin conjugate hydrolase homolog from Arabidopsis suecica. In: Plant Growth Regulation. 2003 ; Vol. 39, No. 2. pp. 175-181.
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abstract = "We have isolated a homolog of the Arabidopsis thaliana IAA amidohydrolase ILR1 from the related species Arabidopsis suecica. Employing PCR primers targeted to the 5′ and 3′ untranslated regions of ILR1, A. suecica produced an amplified, genomic product (sILR1) of 2027 bp. The cloned sILR1 gene was sequenced, and the DNA homology between sILR1 and ILR1 found to be 98{\%}. The sILR1 cDNA was isolated using RT-PCR, cloned and sequenced. The sILR1 cDNA was found to have 98{\%} homology to its homolog ILR1 both at the DNA and amino acid level. The sILR1 mRNA transcript was found to be expressed in A. suecica tissues at ages 5, 10, and 15 days. Additionally, primers, designed to a ∼300 bp domain conserved in bacterial and plant amidohydrolases, were used to amplify a product from seven members of the Brassicaceae family (A. suecica, A. pumila, A. himalaica, A. griffithiana, A. wallichii, Draba nemoroza and Capsella bursa-pastoris), Lycopersicon esculentum, and two monocot species (Zea mays and Allium cepa) using PCR; this result suggests similar genes may be characterized in other species.",
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Campanella, J, Bakllamaja, V, Restieri, T, Vomacka, M, Herron, J, Patterson, M & Shahtaheri, S 2003, 'Isolation of an ILR1 auxin conjugate hydrolase homolog from Arabidopsis suecica', Plant Growth Regulation, vol. 39, no. 2, pp. 175-181. https://doi.org/10.1023/A:1022528318188

Isolation of an ILR1 auxin conjugate hydrolase homolog from Arabidopsis suecica. / Campanella, James; Bakllamaja, Vinela; Restieri, Tracie; Vomacka, Michael; Herron, Jason; Patterson, Megan; Shahtaheri, Shahin.

In: Plant Growth Regulation, Vol. 39, No. 2, 01.02.2003, p. 175-181.

Research output: Contribution to journalArticle

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T1 - Isolation of an ILR1 auxin conjugate hydrolase homolog from Arabidopsis suecica

AU - Campanella, James

AU - Bakllamaja, Vinela

AU - Restieri, Tracie

AU - Vomacka, Michael

AU - Herron, Jason

AU - Patterson, Megan

AU - Shahtaheri, Shahin

PY - 2003/2/1

Y1 - 2003/2/1

N2 - We have isolated a homolog of the Arabidopsis thaliana IAA amidohydrolase ILR1 from the related species Arabidopsis suecica. Employing PCR primers targeted to the 5′ and 3′ untranslated regions of ILR1, A. suecica produced an amplified, genomic product (sILR1) of 2027 bp. The cloned sILR1 gene was sequenced, and the DNA homology between sILR1 and ILR1 found to be 98%. The sILR1 cDNA was isolated using RT-PCR, cloned and sequenced. The sILR1 cDNA was found to have 98% homology to its homolog ILR1 both at the DNA and amino acid level. The sILR1 mRNA transcript was found to be expressed in A. suecica tissues at ages 5, 10, and 15 days. Additionally, primers, designed to a ∼300 bp domain conserved in bacterial and plant amidohydrolases, were used to amplify a product from seven members of the Brassicaceae family (A. suecica, A. pumila, A. himalaica, A. griffithiana, A. wallichii, Draba nemoroza and Capsella bursa-pastoris), Lycopersicon esculentum, and two monocot species (Zea mays and Allium cepa) using PCR; this result suggests similar genes may be characterized in other species.

AB - We have isolated a homolog of the Arabidopsis thaliana IAA amidohydrolase ILR1 from the related species Arabidopsis suecica. Employing PCR primers targeted to the 5′ and 3′ untranslated regions of ILR1, A. suecica produced an amplified, genomic product (sILR1) of 2027 bp. The cloned sILR1 gene was sequenced, and the DNA homology between sILR1 and ILR1 found to be 98%. The sILR1 cDNA was isolated using RT-PCR, cloned and sequenced. The sILR1 cDNA was found to have 98% homology to its homolog ILR1 both at the DNA and amino acid level. The sILR1 mRNA transcript was found to be expressed in A. suecica tissues at ages 5, 10, and 15 days. Additionally, primers, designed to a ∼300 bp domain conserved in bacterial and plant amidohydrolases, were used to amplify a product from seven members of the Brassicaceae family (A. suecica, A. pumila, A. himalaica, A. griffithiana, A. wallichii, Draba nemoroza and Capsella bursa-pastoris), Lycopersicon esculentum, and two monocot species (Zea mays and Allium cepa) using PCR; this result suggests similar genes may be characterized in other species.

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