Live cell plasma membranes do not exhibit a miscibility phase transition over a wide range of temperatures

Il Hyung Lee, Suvrajit Saha, Anirban Polley, Hector Huang, Satyajit Mayor, Madan Rao, Jay T. Groves

Research output: Contribution to journalArticlepeer-review

38 Scopus citations

Abstract

Lipid/cholesterol mixtures derived from cell membranes as well as their synthetic reconstitutions exhibit well-defined miscibility phase transitions and critical phenomena near physiological temperatures. This suggests that lipid/cholesterol-mediated phase separation plays a role in the organization of live cell membranes. However, macroscopic lipid-phase separation is not generally observed in cell membranes, and the degree to which properties of isolated lipid mixtures are preserved in the cell membrane remain unknown. A fundamental property of phase transitions is that the variation of tagged particle diffusion with temperature exhibits an abrupt change as the system passes through the transition, even when the two phases are distributed in a nanometer-scale emulsion. We support this using a variety of Monte Carlo and atomistic simulations on model lipid membrane systems. However, temperature-dependent fluorescence correlation spectroscopy of labeled lipids and membrane-anchored proteins in live cell membranes shows a consistently smooth increase in the diffusion coefficient as a function of temperature. We find no evidence of a discrete miscibility phase transition throughout a wide range of temperatures: 14-37 °C. This contrasts the behavior of giant plasma membrane vesicles (GPMVs) blebbed from the same cells, which do exhibit phase transitions and macroscopic phase separation. Fluorescence lifetime analysis of a DiI probe in both cases reveals a significant environmental difference between the live cell and the GPMV. Taken together, these data suggest the live cell membrane may avoid the miscibility phase transition inherent to its lipid constituents by actively regulating physical parameters, such as tension, in the membrane.

Original languageEnglish
Pages (from-to)4450-4459
Number of pages10
JournalJournal of Physical Chemistry B
Volume119
Issue number12
DOIs
StatePublished - 26 Mar 2015

Fingerprint

Dive into the research topics of 'Live cell plasma membranes do not exhibit a miscibility phase transition over a wide range of temperatures'. Together they form a unique fingerprint.

Cite this