MEC-10 and MEC-19 reduce the neurotoxicity of the MEC-4(d) DEG/ENaC channel in Caenorhabditis elegans

Yushu Chen, Shashank Bharill, Robert O'Hagan, Ehud Y. Isacoff, Martin Chalfie

Research output: Contribution to journalArticleResearchpeer-review

Abstract

The Caenorhabditis elegans DEG/ENaC proteins MEC-4 and MEC-10 transduce gentle touch in the six touch receptor neurons . Gain-of-function mutations of mec-4 and mec-4(d) result in a hyperactive channel and neurodegeneration in vivo. Loss of MEC-6, a putative DEG/ENaC-specific chaperone, and of the similar protein POML-1 suppresses the neurodegeneration caused by a mec-4(d) mutation. We find that mutation of two genes, mec-10 and a new gene mec-19 (previously named C49G9.1), prevents this action of POML-1, allowing the touch receptor neurons to die in poml-1 mec-4(d) animals. The proteins encoded by these genes normally inhibit mec-4(d) neurotoxicity through different mechanisms. MEC-10, a subunit of the mechanosensory transduction channel with MEC-4, inhibits MEC-4(d) activity without affecting MEC-4 expression. In contrast, MEC-19, a membrane protein specific to nematodes, inhibits MEC-4(d) activity and reduces MEC-4 surface expression.

Original languageEnglish
Pages (from-to)1121-1130
Number of pages10
JournalG3: Genes, Genomes, Genetics
Volume6
Issue number4
DOIs
StatePublished - 1 Jan 2016

Fingerprint

Caenorhabditis elegans
Touch
Mutation
Cellular Mechanotransduction
Neurons
Proteins
Genes
Membrane Proteins

Keywords

  • Caenorhabditis elegans
  • DEG/ENaC channels
  • Neurodegeneration
  • Physiological suppressors
  • Touch sensitivity

Cite this

Chen, Yushu ; Bharill, Shashank ; O'Hagan, Robert ; Isacoff, Ehud Y. ; Chalfie, Martin. / MEC-10 and MEC-19 reduce the neurotoxicity of the MEC-4(d) DEG/ENaC channel in Caenorhabditis elegans. In: G3: Genes, Genomes, Genetics. 2016 ; Vol. 6, No. 4. pp. 1121-1130.
@article{457649adb37b4e7b885fd737f50d2494,
title = "MEC-10 and MEC-19 reduce the neurotoxicity of the MEC-4(d) DEG/ENaC channel in Caenorhabditis elegans",
abstract = "The Caenorhabditis elegans DEG/ENaC proteins MEC-4 and MEC-10 transduce gentle touch in the six touch receptor neurons . Gain-of-function mutations of mec-4 and mec-4(d) result in a hyperactive channel and neurodegeneration in vivo. Loss of MEC-6, a putative DEG/ENaC-specific chaperone, and of the similar protein POML-1 suppresses the neurodegeneration caused by a mec-4(d) mutation. We find that mutation of two genes, mec-10 and a new gene mec-19 (previously named C49G9.1), prevents this action of POML-1, allowing the touch receptor neurons to die in poml-1 mec-4(d) animals. The proteins encoded by these genes normally inhibit mec-4(d) neurotoxicity through different mechanisms. MEC-10, a subunit of the mechanosensory transduction channel with MEC-4, inhibits MEC-4(d) activity without affecting MEC-4 expression. In contrast, MEC-19, a membrane protein specific to nematodes, inhibits MEC-4(d) activity and reduces MEC-4 surface expression.",
keywords = "Caenorhabditis elegans, DEG/ENaC channels, Neurodegeneration, Physiological suppressors, Touch sensitivity",
author = "Yushu Chen and Shashank Bharill and Robert O'Hagan and Isacoff, {Ehud Y.} and Martin Chalfie",
year = "2016",
month = "1",
day = "1",
doi = "10.1534/g3.115.023507",
language = "English",
volume = "6",
pages = "1121--1130",
journal = "G3: Genes, Genomes, Genetics",
issn = "2160-1836",
publisher = "Genetics Society of America",
number = "4",

}

MEC-10 and MEC-19 reduce the neurotoxicity of the MEC-4(d) DEG/ENaC channel in Caenorhabditis elegans. / Chen, Yushu; Bharill, Shashank; O'Hagan, Robert; Isacoff, Ehud Y.; Chalfie, Martin.

In: G3: Genes, Genomes, Genetics, Vol. 6, No. 4, 01.01.2016, p. 1121-1130.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - MEC-10 and MEC-19 reduce the neurotoxicity of the MEC-4(d) DEG/ENaC channel in Caenorhabditis elegans

AU - Chen, Yushu

AU - Bharill, Shashank

AU - O'Hagan, Robert

AU - Isacoff, Ehud Y.

AU - Chalfie, Martin

PY - 2016/1/1

Y1 - 2016/1/1

N2 - The Caenorhabditis elegans DEG/ENaC proteins MEC-4 and MEC-10 transduce gentle touch in the six touch receptor neurons . Gain-of-function mutations of mec-4 and mec-4(d) result in a hyperactive channel and neurodegeneration in vivo. Loss of MEC-6, a putative DEG/ENaC-specific chaperone, and of the similar protein POML-1 suppresses the neurodegeneration caused by a mec-4(d) mutation. We find that mutation of two genes, mec-10 and a new gene mec-19 (previously named C49G9.1), prevents this action of POML-1, allowing the touch receptor neurons to die in poml-1 mec-4(d) animals. The proteins encoded by these genes normally inhibit mec-4(d) neurotoxicity through different mechanisms. MEC-10, a subunit of the mechanosensory transduction channel with MEC-4, inhibits MEC-4(d) activity without affecting MEC-4 expression. In contrast, MEC-19, a membrane protein specific to nematodes, inhibits MEC-4(d) activity and reduces MEC-4 surface expression.

AB - The Caenorhabditis elegans DEG/ENaC proteins MEC-4 and MEC-10 transduce gentle touch in the six touch receptor neurons . Gain-of-function mutations of mec-4 and mec-4(d) result in a hyperactive channel and neurodegeneration in vivo. Loss of MEC-6, a putative DEG/ENaC-specific chaperone, and of the similar protein POML-1 suppresses the neurodegeneration caused by a mec-4(d) mutation. We find that mutation of two genes, mec-10 and a new gene mec-19 (previously named C49G9.1), prevents this action of POML-1, allowing the touch receptor neurons to die in poml-1 mec-4(d) animals. The proteins encoded by these genes normally inhibit mec-4(d) neurotoxicity through different mechanisms. MEC-10, a subunit of the mechanosensory transduction channel with MEC-4, inhibits MEC-4(d) activity without affecting MEC-4 expression. In contrast, MEC-19, a membrane protein specific to nematodes, inhibits MEC-4(d) activity and reduces MEC-4 surface expression.

KW - Caenorhabditis elegans

KW - DEG/ENaC channels

KW - Neurodegeneration

KW - Physiological suppressors

KW - Touch sensitivity

UR - http://www.scopus.com/inward/record.url?scp=84963959982&partnerID=8YFLogxK

U2 - 10.1534/g3.115.023507

DO - 10.1534/g3.115.023507

M3 - Article

VL - 6

SP - 1121

EP - 1130

JO - G3: Genes, Genomes, Genetics

JF - G3: Genes, Genomes, Genetics

SN - 2160-1836

IS - 4

ER -