MEC-2 regulates C. elegans DEG/ENaC channels needed for mechanosensation

Miriam B. Goodman, Glen G. Ernstrom, Dattananda S. Chelur, Robert O'Hagan, C. Andrea Yao, Martin Chalfie

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Touch sensitivity in animals relies on nerve endings in the skin that convert mechanical force into electrical signals. In the nematode Caenorhabditis elegans, gentle touch to the body wall is sensed by six mechanosensory neurons that express two amiloride-sensitive Na+ channel proteins (DEG/ENaC). These proteins, MEC-4 and MEC-10, are required for touch sensation and can mutate to cause neuronal degeneration. Here we show that these mutant or 'd' forms of MEC-4 and MEC-10 produce a constitutively active, amiloride-sensitive ionic current when co-expressed in Xenopus oocytes, but not on their own. MEC-2, a stomatin-related protein needed for touch sensitivity, increased the activity of mutant channels about 40-fold and allowed currents to be detected with wild-type MEC-4 and MEC-10. Whereas neither the central, stomatin-like domain of MEC-2 nor human stomatin retained the activity of full-length MEC-2, both produced amiloride-sensitive currents with MEC-4d. Our findings indicate that MEC-2 regulates MEC-4/MEC-10 ion channels and raise the possibility that similar ion channels may be formed by stomatin-like proteins and DEG/ENaC proteins that are co-expressed in both vertebrates and invertebrates. Some of these channels may mediate mechanosensory responses.

Original languageEnglish
Pages (from-to)1039-1042
Number of pages4
JournalNature
Volume415
Issue number6875
DOIs
StatePublished - 28 Feb 2002

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Touch
Amiloride
Proteins
Ion Channels
Nerve Endings
Caenorhabditis elegans
Invertebrates
Xenopus
Oocytes
Vertebrates
Neurons
Skin

Cite this

Goodman, M. B., Ernstrom, G. G., Chelur, D. S., O'Hagan, R., Yao, C. A., & Chalfie, M. (2002). MEC-2 regulates C. elegans DEG/ENaC channels needed for mechanosensation. Nature, 415(6875), 1039-1042. https://doi.org/10.1038/4151039a
Goodman, Miriam B. ; Ernstrom, Glen G. ; Chelur, Dattananda S. ; O'Hagan, Robert ; Yao, C. Andrea ; Chalfie, Martin. / MEC-2 regulates C. elegans DEG/ENaC channels needed for mechanosensation. In: Nature. 2002 ; Vol. 415, No. 6875. pp. 1039-1042.
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Goodman, MB, Ernstrom, GG, Chelur, DS, O'Hagan, R, Yao, CA & Chalfie, M 2002, 'MEC-2 regulates C. elegans DEG/ENaC channels needed for mechanosensation', Nature, vol. 415, no. 6875, pp. 1039-1042. https://doi.org/10.1038/4151039a

MEC-2 regulates C. elegans DEG/ENaC channels needed for mechanosensation. / Goodman, Miriam B.; Ernstrom, Glen G.; Chelur, Dattananda S.; O'Hagan, Robert; Yao, C. Andrea; Chalfie, Martin.

In: Nature, Vol. 415, No. 6875, 28.02.2002, p. 1039-1042.

Research output: Contribution to journalArticleResearchpeer-review

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Goodman MB, Ernstrom GG, Chelur DS, O'Hagan R, Yao CA, Chalfie M. MEC-2 regulates C. elegans DEG/ENaC channels needed for mechanosensation. Nature. 2002 Feb 28;415(6875):1039-1042. https://doi.org/10.1038/4151039a