Mechanistic studies of Microcystic aeruginosa inactivation and degradation by UV-C irradiation and chlorination with poly-synchronous analyses

Huase Ou, Naiyun Gao, Yang Deng, Junlian Qiao, Kejia Zhang, Tian Li, Lei Dong

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

Poly-synchronous techniques were performed to investigate the inactivation and degradation mechanisms of Microcystic aeruginosa under UV-C irradiation and chlorination. Extracellular dissolved organic matter (EDOM) and intracellular dissolved organic matter (IDOM) properties were analyzed using excitation emission matrix (EEM) fluorescence spectroscopy, while the concentration of biochemical parameters including protein, phycocyanin, chlorophyll-a, and microcystin-LR was determined. Transmission electron microscopy was also used to obtained ultrastructural images. EEM analysis revealed that protein-like matters were the major EDOM fluorescence component, while amino acid-like and protein-like matters constituted IDOM with little amount of humic-like substances. In addition, the monitor of biochemical parameters showed that they had different susceptibility under the inactivation reactions. Poly-synchronous techniques confirmed that UV-C irradiation was more appropriate than chlorination for M. aeruginosa inactivation and degradation. The primary mechanism of UV-C irradiation was direct photo-degradation and indirect oxidation by reactive oxygen species, which effectively degraded the fluorescence EDOM and IDOM and caused decomposition of cytoplasmic inclusions and intracellular bioorganic substances. Different from UV-C, the inactivation during chlorination was due to the formation of HOCl, which permeated into the cyanobacteria cells and caused intracellular damage, accompanied with incomplete degradation of IDOM and harmful MC-LR.

Original languageEnglish
Pages (from-to)107-119
Number of pages13
JournalDesalination
Volume272
Issue number1-3
DOIs
StatePublished - 3 May 2011

Fingerprint

Chlorination
chlorination
dissolved organic matter
Biological materials
irradiation
Irradiation
Degradation
degradation
Proteins
protein
fluorescence
Fluorescence
Phycocyanin
matrix
fluorescence spectroscopy
Photodegradation
Fluorescence spectroscopy
Chlorophyll
photodegradation
Amino acids

Keywords

  • Chlorination
  • Fluorescence spectroscopy
  • Microcystic aeruginosa
  • Transmission electron microscopy
  • UV-C irradiation

Cite this

Ou, Huase ; Gao, Naiyun ; Deng, Yang ; Qiao, Junlian ; Zhang, Kejia ; Li, Tian ; Dong, Lei. / Mechanistic studies of Microcystic aeruginosa inactivation and degradation by UV-C irradiation and chlorination with poly-synchronous analyses. In: Desalination. 2011 ; Vol. 272, No. 1-3. pp. 107-119.
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Mechanistic studies of Microcystic aeruginosa inactivation and degradation by UV-C irradiation and chlorination with poly-synchronous analyses. / Ou, Huase; Gao, Naiyun; Deng, Yang; Qiao, Junlian; Zhang, Kejia; Li, Tian; Dong, Lei.

In: Desalination, Vol. 272, No. 1-3, 03.05.2011, p. 107-119.

Research output: Contribution to journalArticle

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