Metabolic events during programmed cell death in insect labial glands.

Reginald Halaby, Z. Zakeri, R. A. Lockshin

Research output: Contribution to journalArticleResearchpeer-review

19 Citations (Scopus)

Abstract

The labial gland of Manduca sexta is a valuable system to study the mechanisms of programmed cell death since the death of the gland is nearly synchronous and, except for the anterior duct, involves all of the tissue. The gland degenerates in 5 days during pupation. Our previous work documents a drop in total protein synthesis as the gland degenerates. To evaluate potential causes of this altered protein synthesis, we monitored several parameters of metabolism in dying cells: levels of adenosine triphosphate to estimate the energy resources of the gland; reduction of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide to assess mitochondrial respiration; levels of acid phosphatase to assay lysosomal enzyme activity; and concentrations of cyclic nucleotides and inositol triphosphate to monitor signaling. While protein synthesis fell precipitously on day 0, total adenosine triphosphate and mitochondrial respiration were unchanged until the cells underwent massive collapse on day 3. Lysosomal acid phosphatase increased during early metamorphosis, and ultimately the bulk of the cytoplasm was destroyed in autophagic vacuoles. Changes in the concentrations of second messengers were modest and late. The relationships between the metabolism and the collapse of the labial gland are under investigation.

Original languageEnglish
Pages (from-to)597-601
Number of pages5
JournalBiochemistry and cell biology = Biochimie et biologie cellulaire
Volume72
Issue number11-12
DOIs
StatePublished - 1 Jan 1994

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Cell death
Lip
Insects
Cell Death
Acid Phosphatase
Metabolism
Respiration
Adenosine Triphosphate
Manduca
Proteins
Cyclic Nucleotides
Enzyme Assays
Enzyme activity
Second Messenger Systems
Inositol
Energy resources
Vacuoles
Ducts
Assays
Cytoplasm

Cite this

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title = "Metabolic events during programmed cell death in insect labial glands.",
abstract = "The labial gland of Manduca sexta is a valuable system to study the mechanisms of programmed cell death since the death of the gland is nearly synchronous and, except for the anterior duct, involves all of the tissue. The gland degenerates in 5 days during pupation. Our previous work documents a drop in total protein synthesis as the gland degenerates. To evaluate potential causes of this altered protein synthesis, we monitored several parameters of metabolism in dying cells: levels of adenosine triphosphate to estimate the energy resources of the gland; reduction of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide to assess mitochondrial respiration; levels of acid phosphatase to assay lysosomal enzyme activity; and concentrations of cyclic nucleotides and inositol triphosphate to monitor signaling. While protein synthesis fell precipitously on day 0, total adenosine triphosphate and mitochondrial respiration were unchanged until the cells underwent massive collapse on day 3. Lysosomal acid phosphatase increased during early metamorphosis, and ultimately the bulk of the cytoplasm was destroyed in autophagic vacuoles. Changes in the concentrations of second messengers were modest and late. The relationships between the metabolism and the collapse of the labial gland are under investigation.",
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Metabolic events during programmed cell death in insect labial glands. / Halaby, Reginald; Zakeri, Z.; Lockshin, R. A.

In: Biochemistry and cell biology = Biochimie et biologie cellulaire, Vol. 72, No. 11-12, 01.01.1994, p. 597-601.

Research output: Contribution to journalArticleResearchpeer-review

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