Molecular genetic evidence suggests Long Island as the geographic origin for the present population of Bay scallops in Barnegat Bay, New Jersey

James Campanella, Paul Bologna, L. E J Kim, John V. Smalley

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

We have used molecular genetic methods to examine the question of the geographic origins of the newly returned Argopecten irradians populations in Barnegat Bay (BB), New Jersey. Using PCR to amplify specific polymorphic microsatellite regions for bay scallop, we have genetically compared the 2004 and 2005 BB populations to those from Long Island (LI), New York, and North Carolina (NC). Our studies indicate that the 2004 and 2005 BB populations are genetically similar with some allelic frequency differences. Five of the eight loci studied are identical for marker size among BB, LI, and NC populations. The S336 locus demonstrates polymorphic sequences of 138 and 158 basepairs in the NC population that are not observed in LI or BB. The CI832 locus appears identical (122 basepairs) between LI and BB, but demonstrates polymorphisms (132 or 142 basepairs) in the NC population. Additionally, the NC group manifests two further alleles in the M26 locus (135 and 149 basepairs) not seen in BB or LI. These results, along with genetic distance and mean estimated gene flow calculations, support a physical transfer of the Long Island bay scallop larvae down the Atlantic coast to the transition regions around Barnegat Bay.

Original languageEnglish
Pages (from-to)303-306
Number of pages4
JournalJournal of Shellfish Research
Volume26
Issue number2
DOIs
StatePublished - 1 Aug 2007

Fingerprint

Argopecten irradians
molecular genetics
provenance
loci
gene frequency
genetic distance
gene flow
genetic polymorphism
microsatellite repeats
alleles
coasts
larvae
allele
polymorphism

Keywords

  • Argopecten irradians
  • Bay scallops
  • DNA fingerprinting
  • Microsatellite markers
  • Phylogenetic analysis
  • Sequence length polymorphisms

Cite this

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title = "Molecular genetic evidence suggests Long Island as the geographic origin for the present population of Bay scallops in Barnegat Bay, New Jersey",
abstract = "We have used molecular genetic methods to examine the question of the geographic origins of the newly returned Argopecten irradians populations in Barnegat Bay (BB), New Jersey. Using PCR to amplify specific polymorphic microsatellite regions for bay scallop, we have genetically compared the 2004 and 2005 BB populations to those from Long Island (LI), New York, and North Carolina (NC). Our studies indicate that the 2004 and 2005 BB populations are genetically similar with some allelic frequency differences. Five of the eight loci studied are identical for marker size among BB, LI, and NC populations. The S336 locus demonstrates polymorphic sequences of 138 and 158 basepairs in the NC population that are not observed in LI or BB. The CI832 locus appears identical (122 basepairs) between LI and BB, but demonstrates polymorphisms (132 or 142 basepairs) in the NC population. Additionally, the NC group manifests two further alleles in the M26 locus (135 and 149 basepairs) not seen in BB or LI. These results, along with genetic distance and mean estimated gene flow calculations, support a physical transfer of the Long Island bay scallop larvae down the Atlantic coast to the transition regions around Barnegat Bay.",
keywords = "Argopecten irradians, Bay scallops, DNA fingerprinting, Microsatellite markers, Phylogenetic analysis, Sequence length polymorphisms",
author = "James Campanella and Paul Bologna and Kim, {L. E J} and Smalley, {John V.}",
year = "2007",
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T1 - Molecular genetic evidence suggests Long Island as the geographic origin for the present population of Bay scallops in Barnegat Bay, New Jersey

AU - Campanella, James

AU - Bologna, Paul

AU - Kim, L. E J

AU - Smalley, John V.

PY - 2007/8/1

Y1 - 2007/8/1

N2 - We have used molecular genetic methods to examine the question of the geographic origins of the newly returned Argopecten irradians populations in Barnegat Bay (BB), New Jersey. Using PCR to amplify specific polymorphic microsatellite regions for bay scallop, we have genetically compared the 2004 and 2005 BB populations to those from Long Island (LI), New York, and North Carolina (NC). Our studies indicate that the 2004 and 2005 BB populations are genetically similar with some allelic frequency differences. Five of the eight loci studied are identical for marker size among BB, LI, and NC populations. The S336 locus demonstrates polymorphic sequences of 138 and 158 basepairs in the NC population that are not observed in LI or BB. The CI832 locus appears identical (122 basepairs) between LI and BB, but demonstrates polymorphisms (132 or 142 basepairs) in the NC population. Additionally, the NC group manifests two further alleles in the M26 locus (135 and 149 basepairs) not seen in BB or LI. These results, along with genetic distance and mean estimated gene flow calculations, support a physical transfer of the Long Island bay scallop larvae down the Atlantic coast to the transition regions around Barnegat Bay.

AB - We have used molecular genetic methods to examine the question of the geographic origins of the newly returned Argopecten irradians populations in Barnegat Bay (BB), New Jersey. Using PCR to amplify specific polymorphic microsatellite regions for bay scallop, we have genetically compared the 2004 and 2005 BB populations to those from Long Island (LI), New York, and North Carolina (NC). Our studies indicate that the 2004 and 2005 BB populations are genetically similar with some allelic frequency differences. Five of the eight loci studied are identical for marker size among BB, LI, and NC populations. The S336 locus demonstrates polymorphic sequences of 138 and 158 basepairs in the NC population that are not observed in LI or BB. The CI832 locus appears identical (122 basepairs) between LI and BB, but demonstrates polymorphisms (132 or 142 basepairs) in the NC population. Additionally, the NC group manifests two further alleles in the M26 locus (135 and 149 basepairs) not seen in BB or LI. These results, along with genetic distance and mean estimated gene flow calculations, support a physical transfer of the Long Island bay scallop larvae down the Atlantic coast to the transition regions around Barnegat Bay.

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