Population structure of colonizing and invasive staphylococcus aureus strains in northern Vietnam

Bich Ngoc Thi Vu, Alexander J. Jafari, Matthew Aardema, Huong Kieu Thi Tran, Diep Ngoc Thi Nguyen, Trinh Tuyet Dao, Trung Vu Nguyen, Toan Khanh Tran, Chuc Kim Thi Nguyen, Annette Fox, Anne Laure Bañuls, Guy Thwaites, Kinh Van Nguyen, Heiman F.L. Wertheim

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Staphylococcus aureus is an important global health problem worldwide. There is still scarce information on the population structure of S. aureus strains in Asia, where the majority of the world population lives. This study characterized the diversity of S. aureus strains in northern Vietnam through multilocus sequence typing (MLST). Eighty-five carriage isolates from the community and 77 invasive isolates from the clinical setting were selected and tested for meticillin resistance and the presence of Panton-Valentine leukocidin (PVL). MLST was performed on these isolates, of which CC59 (25.4 %), CC188 (17.3 %) and CC45 (16.7 %) were the predominant clonal complexes (CCs). CC59 carriage isolates had significantly lower rates of meticillin-resistant S. aureus (MRSA) than their corresponding clinical group isolates (32 vs 83 %). There were no significant differences in rates of MRSA between carriage isolates and clinical isolates of CC45 and CC188. CC59 carriage isolates were significantly lower in rates of PVL+ than CC59 clinical isolates (32 vs 83 %), but the converse was shown in CC45 isolates (14 vs 0 %, respectively). This study revealed vast differences in the molecular epidemiology and population structure of S. aureus in community and clinical settings in Vietnam. Nevertheless, the data underline the spread of virulent and/or resistant strains (MRSA and/or PVL+) in the community, suggesting the necessity for further surveillance to determine the mechanism of transmission of these strains (i.e. MRSA/PVL+) outside clinical settings.

Original languageEnglish
Pages (from-to)298-305
Number of pages8
JournalJournal of Medical Microbiology
Volume65
Issue number4
DOIs
StatePublished - 1 Apr 2016

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Vietnam
Staphylococcus aureus
Methicillin
Population
Multilocus Sequence Typing
Molecular Epidemiology
Panton-Valentine leukocidin

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Vu, B. N. T., Jafari, A. J., Aardema, M., Tran, H. K. T., Nguyen, D. N. T., Dao, T. T., ... Wertheim, H. F. L. (2016). Population structure of colonizing and invasive staphylococcus aureus strains in northern Vietnam. Journal of Medical Microbiology, 65(4), 298-305. https://doi.org/10.1099/jmm.0.000220
Vu, Bich Ngoc Thi ; Jafari, Alexander J. ; Aardema, Matthew ; Tran, Huong Kieu Thi ; Nguyen, Diep Ngoc Thi ; Dao, Trinh Tuyet ; Nguyen, Trung Vu ; Tran, Toan Khanh ; Nguyen, Chuc Kim Thi ; Fox, Annette ; Bañuls, Anne Laure ; Thwaites, Guy ; Van Nguyen, Kinh ; Wertheim, Heiman F.L. / Population structure of colonizing and invasive staphylococcus aureus strains in northern Vietnam. In: Journal of Medical Microbiology. 2016 ; Vol. 65, No. 4. pp. 298-305.
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abstract = "Staphylococcus aureus is an important global health problem worldwide. There is still scarce information on the population structure of S. aureus strains in Asia, where the majority of the world population lives. This study characterized the diversity of S. aureus strains in northern Vietnam through multilocus sequence typing (MLST). Eighty-five carriage isolates from the community and 77 invasive isolates from the clinical setting were selected and tested for meticillin resistance and the presence of Panton-Valentine leukocidin (PVL). MLST was performed on these isolates, of which CC59 (25.4 {\%}), CC188 (17.3 {\%}) and CC45 (16.7 {\%}) were the predominant clonal complexes (CCs). CC59 carriage isolates had significantly lower rates of meticillin-resistant S. aureus (MRSA) than their corresponding clinical group isolates (32 vs 83 {\%}). There were no significant differences in rates of MRSA between carriage isolates and clinical isolates of CC45 and CC188. CC59 carriage isolates were significantly lower in rates of PVL+ than CC59 clinical isolates (32 vs 83 {\%}), but the converse was shown in CC45 isolates (14 vs 0 {\%}, respectively). This study revealed vast differences in the molecular epidemiology and population structure of S. aureus in community and clinical settings in Vietnam. Nevertheless, the data underline the spread of virulent and/or resistant strains (MRSA and/or PVL+) in the community, suggesting the necessity for further surveillance to determine the mechanism of transmission of these strains (i.e. MRSA/PVL+) outside clinical settings.",
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Vu, BNT, Jafari, AJ, Aardema, M, Tran, HKT, Nguyen, DNT, Dao, TT, Nguyen, TV, Tran, TK, Nguyen, CKT, Fox, A, Bañuls, AL, Thwaites, G, Van Nguyen, K & Wertheim, HFL 2016, 'Population structure of colonizing and invasive staphylococcus aureus strains in northern Vietnam', Journal of Medical Microbiology, vol. 65, no. 4, pp. 298-305. https://doi.org/10.1099/jmm.0.000220

Population structure of colonizing and invasive staphylococcus aureus strains in northern Vietnam. / Vu, Bich Ngoc Thi; Jafari, Alexander J.; Aardema, Matthew; Tran, Huong Kieu Thi; Nguyen, Diep Ngoc Thi; Dao, Trinh Tuyet; Nguyen, Trung Vu; Tran, Toan Khanh; Nguyen, Chuc Kim Thi; Fox, Annette; Bañuls, Anne Laure; Thwaites, Guy; Van Nguyen, Kinh; Wertheim, Heiman F.L.

In: Journal of Medical Microbiology, Vol. 65, No. 4, 01.04.2016, p. 298-305.

Research output: Contribution to journalArticle

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T1 - Population structure of colonizing and invasive staphylococcus aureus strains in northern Vietnam

AU - Vu, Bich Ngoc Thi

AU - Jafari, Alexander J.

AU - Aardema, Matthew

AU - Tran, Huong Kieu Thi

AU - Nguyen, Diep Ngoc Thi

AU - Dao, Trinh Tuyet

AU - Nguyen, Trung Vu

AU - Tran, Toan Khanh

AU - Nguyen, Chuc Kim Thi

AU - Fox, Annette

AU - Bañuls, Anne Laure

AU - Thwaites, Guy

AU - Van Nguyen, Kinh

AU - Wertheim, Heiman F.L.

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