Protein disulfide engineering

Alan A. Dombkowski, Kazi Zakia Sultana, Douglas B. Craig

Research output: Contribution to journalReview articleResearchpeer-review

62 Citations (Scopus)

Abstract

Improving the stability of proteins is an important goal in many biomedical and industrial applications. A logical approach is to emulate stabilizing molecular interactions found in nature. Disulfide bonds are covalent interactions that provide substantial stability to many proteins and conform to well-defined geometric conformations, thus making them appealing candidates in protein engineering efforts. Disulfide engineering is the directed design of novel disulfide bonds into target proteins. This important biotechnological tool has achieved considerable success in a wide range of applications, yet the rules that govern the stabilizing effects of disulfide bonds are not fully characterized. Contrary to expectations, many designed disulfide bonds have resulted in decreased stability of the modified protein. We review progress in disulfide engineering, with an emphasis on the issue of stability and computational methods that facilitate engineering efforts.

Original languageEnglish
Pages (from-to)206-212
Number of pages7
JournalFEBS Letters
Volume588
Issue number2
DOIs
StatePublished - 21 Jan 2014

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Protein Engineering
Disulfides
Proteins
Protein Stability
Covalent bonds
Molecular interactions
Computational methods
Industrial applications
Conformations

Keywords

  • Disulfide
  • Engineering
  • Kinetics
  • Protein
  • Stability
  • Thermodynamics

Cite this

Dombkowski, Alan A. ; Sultana, Kazi Zakia ; Craig, Douglas B. / Protein disulfide engineering. In: FEBS Letters. 2014 ; Vol. 588, No. 2. pp. 206-212.
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Dombkowski, AA, Sultana, KZ & Craig, DB 2014, 'Protein disulfide engineering', FEBS Letters, vol. 588, no. 2, pp. 206-212. https://doi.org/10.1016/j.febslet.2013.11.024

Protein disulfide engineering. / Dombkowski, Alan A.; Sultana, Kazi Zakia; Craig, Douglas B.

In: FEBS Letters, Vol. 588, No. 2, 21.01.2014, p. 206-212.

Research output: Contribution to journalReview articleResearchpeer-review

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