A guanine nucleotide exchange factor (GEF)1 1 The abbreviations used are: eIF-2, eukaryotic initiation factor 2; GEF, GDP exchange factor; HCI, heme-controlled translational inhibitor (an eIF-2α kinase); DAI, double-stranded (ds) RNA-activated translational inhibitor (another eIF-2α kinase); Met-tRNAi, eukaryotic initiator methionyl-tRNA; SDS-PAGE, sodium dodecylsulfate-polyacrylamide gel electrophoresis., catalyzing the exchange of GDP bound to initiation factor eIF-2 for GTP, has been isolated from S3 HeLa cells as the eIF-2·GEF complex and extensively purified by procedures originally developed for purification of GEF from rabbit reticulocytes. The HeLa cell factor resembles rabbit reticulocyte eIF-2·GEF in polypeptide composition, catalytic activity, and inactivation by α-phosphorylated eIF-2.
|Number of pages||7|
|Journal||Biochemical and Biophysical Research Communications|
|State||Published - 13 Feb 1986|