Purification and properties of the guanine nucleotide exchange factor (GEF) from HeLa cells and its role in the initiation of protein synthesis

Thomas M. Mariano; John Siekierka; Severo Ochoa

doi:10.1016/0006-291X(86)90372-4

Purification and properties of the guanine nucleotide exchange factor (GEF) from HeLa cells and its role in the initiation of protein synthesis

Thomas M. Mariano
, John Siekierka
, Severo Ochoa

Chemistry and Biochemistry

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

A guanine nucleotide exchange factor (GEF)¹ 1 The abbreviations used are: eIF-2, eukaryotic initiation factor 2; GEF, GDP exchange factor; HCI, heme-controlled translational inhibitor (an eIF-2α kinase); DAI, double-stranded (ds) RNA-activated translational inhibitor (another eIF-2α kinase); Met-tRNA_i, eukaryotic initiator methionyl-tRNA; SDS-PAGE, sodium dodecylsulfate-polyacrylamide gel electrophoresis., catalyzing the exchange of GDP bound to initiation factor eIF-2 for GTP, has been isolated from S3 HeLa cells as the eIF-2·GEF complex and extensively purified by procedures originally developed for purification of GEF from rabbit reticulocytes. The HeLa cell factor resembles rabbit reticulocyte eIF-2·GEF in polypeptide composition, catalytic activity, and inactivation by α-phosphorylated eIF-2.

Original language	English
Pages (from-to)	1160-1166
Number of pages	7
Journal	Biochemical and Biophysical Research Communications
Volume	134
Issue number	3
DOIs	https://doi.org/10.1016/0006-291X(86)90372-4
State	Published - 13 Feb 1986

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title = "Purification and properties of the guanine nucleotide exchange factor (GEF) from HeLa cells and its role in the initiation of protein synthesis",

abstract = "A guanine nucleotide exchange factor (GEF)1 1 The abbreviations used are: eIF-2, eukaryotic initiation factor 2; GEF, GDP exchange factor; HCI, heme-controlled translational inhibitor (an eIF-2α kinase); DAI, double-stranded (ds) RNA-activated translational inhibitor (another eIF-2α kinase); Met-tRNAi, eukaryotic initiator methionyl-tRNA; SDS-PAGE, sodium dodecylsulfate-polyacrylamide gel electrophoresis., catalyzing the exchange of GDP bound to initiation factor eIF-2 for GTP, has been isolated from S3 HeLa cells as the eIF-2·GEF complex and extensively purified by procedures originally developed for purification of GEF from rabbit reticulocytes. The HeLa cell factor resembles rabbit reticulocyte eIF-2·GEF in polypeptide composition, catalytic activity, and inactivation by α-phosphorylated eIF-2.",

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T1 - Purification and properties of the guanine nucleotide exchange factor (GEF) from HeLa cells and its role in the initiation of protein synthesis

AU - Mariano, Thomas M.

AU - Siekierka, John

AU - Ochoa, Severo

PY - 1986/2/13

Y1 - 1986/2/13

N2 - A guanine nucleotide exchange factor (GEF)1 1 The abbreviations used are: eIF-2, eukaryotic initiation factor 2; GEF, GDP exchange factor; HCI, heme-controlled translational inhibitor (an eIF-2α kinase); DAI, double-stranded (ds) RNA-activated translational inhibitor (another eIF-2α kinase); Met-tRNAi, eukaryotic initiator methionyl-tRNA; SDS-PAGE, sodium dodecylsulfate-polyacrylamide gel electrophoresis., catalyzing the exchange of GDP bound to initiation factor eIF-2 for GTP, has been isolated from S3 HeLa cells as the eIF-2·GEF complex and extensively purified by procedures originally developed for purification of GEF from rabbit reticulocytes. The HeLa cell factor resembles rabbit reticulocyte eIF-2·GEF in polypeptide composition, catalytic activity, and inactivation by α-phosphorylated eIF-2.

AB - A guanine nucleotide exchange factor (GEF)1 1 The abbreviations used are: eIF-2, eukaryotic initiation factor 2; GEF, GDP exchange factor; HCI, heme-controlled translational inhibitor (an eIF-2α kinase); DAI, double-stranded (ds) RNA-activated translational inhibitor (another eIF-2α kinase); Met-tRNAi, eukaryotic initiator methionyl-tRNA; SDS-PAGE, sodium dodecylsulfate-polyacrylamide gel electrophoresis., catalyzing the exchange of GDP bound to initiation factor eIF-2 for GTP, has been isolated from S3 HeLa cells as the eIF-2·GEF complex and extensively purified by procedures originally developed for purification of GEF from rabbit reticulocytes. The HeLa cell factor resembles rabbit reticulocyte eIF-2·GEF in polypeptide composition, catalytic activity, and inactivation by α-phosphorylated eIF-2.

UR - https://www.scopus.com/pages/publications/0022577992

U2 - 10.1016/0006-291X(86)90372-4

DO - 10.1016/0006-291X(86)90372-4

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SN - 0006-291X

VL - 134

SP - 1160

EP - 1166

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 3

ER -

Purification and properties of the guanine nucleotide exchange factor (GEF) from HeLa cells and its role in the initiation of protein synthesis

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