Radioiodination of interleukin 2 to high specific activities by the vapor-phase chloramine T method

John J. Siekierka, Steven DeGudicibus

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Recombinant human interleukin 2 (IL-2) was radioiodinated utilizing the vapor phase chloramine T method of iodination. The method is rapid, reproducible, and allows the efficient radioiodination of IL-2 to specific activities higher than those previously attained with full retention of biological activity. IL-2 radioiodinated by this method binds with high affinity to receptors present on phytohemagglutinin-stimulated peripheral blood lymphocytes and should be useful for the study of receptor structure and function.

Original languageEnglish
Pages (from-to)514-517
Number of pages4
JournalAnalytical Biochemistry
Volume172
Issue number2
DOIs
StatePublished - 1 Aug 1988

Fingerprint

Interleukin-2
Vapors
Lymphocytes
Halogenation
Phytohemagglutinins
Bioactivity
Blood
chloramine-T

Keywords

  • interleukin 2
  • interleukin 2 receptor
  • lymphokines
  • radioiodination

Cite this

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Radioiodination of interleukin 2 to high specific activities by the vapor-phase chloramine T method. / Siekierka, John J.; DeGudicibus, Steven.

In: Analytical Biochemistry, Vol. 172, No. 2, 01.08.1988, p. 514-517.

Research output: Contribution to journalArticle

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