Radioiodination of interleukin 2 to high specific activities by the vapor-phase chloramine T method

John J. Siekierka; Steven DeGudicibus

doi:10.1016/0003-2697(88)90477-0

Radioiodination of interleukin 2 to high specific activities by the vapor-phase chloramine T method

John J. Siekierka, Steven DeGudicibus

Chemistry and Biochemistry

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3 Scopus citations

Abstract

Recombinant human interleukin 2 (IL-2) was radioiodinated utilizing the vapor phase chloramine T method of iodination. The method is rapid, reproducible, and allows the efficient radioiodination of IL-2 to specific activities higher than those previously attained with full retention of biological activity. IL-2 radioiodinated by this method binds with high affinity to receptors present on phytohemagglutinin-stimulated peripheral blood lymphocytes and should be useful for the study of receptor structure and function.

Original language	English
Pages (from-to)	514-517
Number of pages	4
Journal	Analytical Biochemistry
Volume	172
Issue number	2
DOIs	https://doi.org/10.1016/0003-2697(88)90477-0
State	Published - 1 Aug 1988

Keywords

interleukin 2
interleukin 2 receptor
lymphokines
radioiodination

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10.1016/0003-2697(88)90477-0

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title = "Radioiodination of interleukin 2 to high specific activities by the vapor-phase chloramine T method",

abstract = "Recombinant human interleukin 2 (IL-2) was radioiodinated utilizing the vapor phase chloramine T method of iodination. The method is rapid, reproducible, and allows the efficient radioiodination of IL-2 to specific activities higher than those previously attained with full retention of biological activity. IL-2 radioiodinated by this method binds with high affinity to receptors present on phytohemagglutinin-stimulated peripheral blood lymphocytes and should be useful for the study of receptor structure and function.",

keywords = "interleukin 2, interleukin 2 receptor, lymphokines, radioiodination",

author = "Siekierka, \{John J.\} and Steven DeGudicibus",

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T1 - Radioiodination of interleukin 2 to high specific activities by the vapor-phase chloramine T method

AU - Siekierka, John J.

AU - DeGudicibus, Steven

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N2 - Recombinant human interleukin 2 (IL-2) was radioiodinated utilizing the vapor phase chloramine T method of iodination. The method is rapid, reproducible, and allows the efficient radioiodination of IL-2 to specific activities higher than those previously attained with full retention of biological activity. IL-2 radioiodinated by this method binds with high affinity to receptors present on phytohemagglutinin-stimulated peripheral blood lymphocytes and should be useful for the study of receptor structure and function.

AB - Recombinant human interleukin 2 (IL-2) was radioiodinated utilizing the vapor phase chloramine T method of iodination. The method is rapid, reproducible, and allows the efficient radioiodination of IL-2 to specific activities higher than those previously attained with full retention of biological activity. IL-2 radioiodinated by this method binds with high affinity to receptors present on phytohemagglutinin-stimulated peripheral blood lymphocytes and should be useful for the study of receptor structure and function.

KW - interleukin 2

KW - interleukin 2 receptor

KW - lymphokines

KW - radioiodination

UR - https://www.scopus.com/pages/publications/0023765919

U2 - 10.1016/0003-2697(88)90477-0

DO - 10.1016/0003-2697(88)90477-0

M3 - Article

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SN - 0003-2697

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EP - 517

JO - Analytical Biochemistry

JF - Analytical Biochemistry

IS - 2

ER -

Radioiodination of interleukin 2 to high specific activities by the vapor-phase chloramine T method

Abstract

Keywords

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