Regulation of a chitinase gene promoter by ethylene and elicitors in bean protoplasts

Dominique Roby, Karen Brogue, John Gaynor, Richard Broglie

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

Chitinase gene expression has been shown to be transcriptionally regulated by a number of inducers, including ethylene, elicitors, and pathogen attack. To investigate the mechanism(s) responsible for induction of chitinase gene expression in response to various stimuli, we have developed a transient gene expression system in bean (Phaseolus vulgaris) protoplasts that is responsive to ethylene and elicitor treatment. This system was used to study the expression of a chimeric gene composed of the 5′ flanking sequences of a bean endochitinase gene fused to the reporter gene β-glucuronidase linked to a 3′ fragment from nopaline synthase. Addition of 1-aminocyclopropane-1-carboxylic acid, the direct precursor of ethylene, or elicitors such as chitin oligosaccharides or cell wall fragments derived from Colletotrichum lagenarium, to transformed protoplasts resulted in a rapid and marked increase in the expression of the chimeric gene. The kinetics and dose response for these treatments were similar to those observed for the native gene in vivo. Analyses of 5′ deletion mutants in the protoplast system indicated that DMA sequences located between -305 and -236 are important for both ethylene and elicitor induction of the reporter gene.

Original languageEnglish
Pages (from-to)433-439
Number of pages7
JournalPlant Physiology
Volume97
Issue number1
DOIs
StatePublished - 1 Jan 1991

Fingerprint

Chitinases
Protoplasts
chitinase
protoplasts
ethylene
beans
promoter regions
Gene Expression
Reporter Genes
reporter genes
gene expression
Genes
genes
Colletotrichum orbiculare
Colletotrichum
nopaline
Chitin
Phaseolus
5' Flanking Region
1-aminocyclopropane-1-carboxylic acid

Cite this

Roby, Dominique ; Brogue, Karen ; Gaynor, John ; Broglie, Richard. / Regulation of a chitinase gene promoter by ethylene and elicitors in bean protoplasts. In: Plant Physiology. 1991 ; Vol. 97, No. 1. pp. 433-439.
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Regulation of a chitinase gene promoter by ethylene and elicitors in bean protoplasts. / Roby, Dominique; Brogue, Karen; Gaynor, John; Broglie, Richard.

In: Plant Physiology, Vol. 97, No. 1, 01.01.1991, p. 433-439.

Research output: Contribution to journalArticle

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